chapter 12 Gene can be cloned in recombinant plasmids Flashcards

1
Q

Define the definition of recombinant DNA

A

formed when combining pieces of DNA from two different sources often different species to form a single DNA molecule.

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2
Q

small, cir laboratory DNA molecules that replicate (duplicate) separately from the much larger bacterial chromosome.

A

plasmid

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3
Q

does plasmids are easily manipulated to carry virtual any genes , they are key tools for DNA cloning, the production of many identical copies of a target segment of DNA.

A

yes.this is very true plasmid does do the following.

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4
Q

the cutting tools are bacterial enzymes

A

restriction enzymes

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5
Q

restriction site

A

each of which recognizes a particular short DNA sequence

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6
Q

what happens after a restriction enzyme binds to its restriction site

A

it cuts both strands of the DNA at precise points within the sequence (like a pair of highly specific molecular scissors-yielding pieces of DNA called restriction fragments)

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7
Q

What will always cut the all the copies of a particular DNA molecule at the same place

A

same restriction enzyme

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8
Q

complementary molecule is called a nucleic probe because…..

A

it is used to find a speciic gene or other nucleotide sequence within a mass of DNA.

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