chapter 12 Gene can be cloned in recombinant plasmids Flashcards
Define the definition of recombinant DNA
formed when combining pieces of DNA from two different sources often different species to form a single DNA molecule.
small, cir laboratory DNA molecules that replicate (duplicate) separately from the much larger bacterial chromosome.
plasmid
does plasmids are easily manipulated to carry virtual any genes , they are key tools for DNA cloning, the production of many identical copies of a target segment of DNA.
yes.this is very true plasmid does do the following.
the cutting tools are bacterial enzymes
restriction enzymes
restriction site
each of which recognizes a particular short DNA sequence
what happens after a restriction enzyme binds to its restriction site
it cuts both strands of the DNA at precise points within the sequence (like a pair of highly specific molecular scissors-yielding pieces of DNA called restriction fragments)
What will always cut the all the copies of a particular DNA molecule at the same place
same restriction enzyme
complementary molecule is called a nucleic probe because…..
it is used to find a speciic gene or other nucleotide sequence within a mass of DNA.