Chapter 12 Flashcards
1
Q
Template Strand
A
Model for synthesis of daughter strand
2
Q
Semiconservative Replication
A
-DNA from parent cell conserved in daughter cell
3
Q
Addition of nucleotides
A
- Always nucleotide triphosphate groups that attaches to the 3’ hydroxyl
- When attached the two outermost phosphate groups diffuse away
4
Q
DNA Polymerase (5)
A
- Enzyme that is a critical component of a large protein complex that carries out DNA Replication
- Cannot initiate polymer synthesis
- Catalyzes attachment of incoming nucleotide to 3’
- Requires a template
- Proofreads
5
Q
leading strand
A
can be synthesized as one continuous polymer
6
Q
lagging strand
A
synthesized in short discontinuous pieces
7
Q
Primer
A
-RNA that starts DNA synthesis
8
Q
proofreading
A
Can correct errors
9
Q
Helicase
A
Unwinds potential double helix at replication fork
10
Q
Single Stranded Binding Proteins
A
- Bind single stranded regions of DNA
- Keeps DNA separated
- Kicked off by DNA polymerase
11
Q
DNA Replication
A
- Process of DNA synthesis in S phase
- DNA directed DNA synthesis
12
Q
Transcription vs. DNA Replication
A
- Transcription makes RNA and copies pieces of genome
- DNA Replication makes DNA and copies entire genome
13
Q
Replication Fork
A
Where genetic info splits
14
Q
Origin of Replication
A
Daughter Strand simultaneously synthesized in 2 directions
15
Q
Replication Bubble
A
Where DNA is replicated
