Chapter 1 - Introduction to Microbiology Flashcards

1
Q

In what year did the CDC begin recording flu deaths for children?

A

2003

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are two reasons this year’s flu season is “odd”?

A
  1. The flu started earlier and does not seem to have reached its peak
  2. The flu is dominated by influenza B, which usually occurs later and at lower rates; this version is known to be deadlier for children and adolescents
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Who received the Nobel Prize for discovering pencillin and even warned about antibiotic resistance?

A

Alexander Fleming

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is a microbe or microorganism?

A

An organism that is too small to visualize with the naked eye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Most microorganism are ____________________ and include all life forms other than plants and animals.

A

Microscopic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the average size of a microorganism?

A

Approximately 0.2 mm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

True or false: all microbes are unicellular.

A

False. Most microbes are unicellular, but they can be multicellular; however, when multicellular, they lack highly differentiated tissues

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What seems to have been an evolutionary invention of plants and animals alone?

A

Tissue differentation and organ specialization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

When did microbal life originate?

A

Shortly after the formation of earth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How old do cosmologists believe earth to be?

A

Approximately 4.5 bilion years old

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

When did the earth become habitable for life?

A

Approximately 4.0 billion years ago once the earth cooled to temperatures less than 100 degrees celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

How old are the oldest rocks?

A

3.8 billion years old

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How old are the earliest known microbial fossils?

A

Approximately 3.4 to 3.5 billion years ago

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the study of microscopic fossils called?

A

Micropaleontology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

In Western Australia, rocks as old as ___________ billion years old were found, and it was believed that fossilized microbes were present in the rock; however, more recently, in the _________ ___________ _________ in Western Australia, it is believed that earliest life arose around 3.43 billion years ago.

A

3.46 billion years ago

Strelley Pool Formation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Why did researchers believe that the earliest signs of microbial life arose earlier than previously thought (i.e., the Strelley Pool Formation in Western Australia)?

A

Researchers outlined in “Microfossils of sulphur-metabolizing cells in 3.4-billion-year-old rocks of Western Australia” that microbial cells had been found based on “indicators of biological affinity,” including hollow cell lumens, carbonaceous cell walls enriched in nitrogen, taphonomic degradation, organization into chains and clusters, and δ13C values of −33 to −46‰ Vienna PeeDee Belemnite (VPDB).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Currently, research suggests that the earliest evidence of microbial life is _____________ billion years ago.

A

3.43 billion years ago

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

There are two fundamentally different types of cells: prokaryotic and eukaryotic. How does one distinguish them?

A

Eukaryotic cells have a membrane-bound nucleus whereas prokaryotic cell; it is the absence or presene of a membrane-bound nucleus is the only true defining feature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Which group of cells are generally specialized for rapid growth and maximal use of nutrients for increasing biomass? Prokaryotes and eukaryotes?

A

Prokaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Which cell types are generally and intially specialized for predation on existing biomass? Prokaryotes or eukaryotes?

A

Eukaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Although there are many exceptions, it is probably fair to think of the ____________________ as specialists at rapid growth at the expense of nutrients dissolved in the water that surrounds them. This has entailed the evolution of small, relatively simple cells with highly effective permease systems to take nutrients from a dilute solution, and a highly effiecient, tightly regulated _______________ to make the most of them.

A

Prokaryotes

Metabolism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What likely drove the multicellularity and complexity of eukaryotes?

A

Predartion of smaller, simpler prokaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Are microbes rare or ubiquitous?

A

Ubiquitous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

There are approximately 10__ human cells on the human body but approximately 10__ microbial cells!

A

1013 human cells

1014 microbial cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
What is the difference between a microbiota and a microbiome?
**A microbiome is the collection of genomes from all the microorganisms found in a particular environment**. Humans, plants, and other animals all have microbiomes; these can be generalised to their entire organism, or broken down into specific microbiomes for different locations on them. **Microbiota**, on the other hand, usually **refers to specific microorganisms that are found within a specific environmen**t. Microbiota can refer to all the microorganisms found in an environment, including bacteria, viruses, and fungi. This means that there are localised differences in the microbiota of each person, depending on where in the body the microbiota is collected from. In each person, their gut microbiota can be radically different to their skin microbiota – care needs to be taken when talking about where microbiota come from.
26
Collectively the microbal biomass is greater than or lesser than the macroscopic biomass by mass on earth.
Greather than
27
Bacteria, fungi, and viruses outnumber human cells in the body by a factor of ___ to \_\_\_.
10 to 1
28
In the human lungs, there are \_\_\_\_+ microbial species; in the gastrointestinal tract, there are _____ microbial species; and in the urogenital tract, there are ____ species of microbial species.
600 1,000 60
29
The tree of life is almost entirely \_\_\_\_\_\_\_\_\_\_\_\_\_\_.
Microbial
30
There are three major lineages of life on earth. What are they?
Eubacteria Archaea Eukarya
31
Eukarya are most biochemically similar to Eubacteria or Archaea?
Archaea
32
A ______________ \_\_\_\_\_\_\_\_\_\_ is a diagram that represents evolutionary relationships among organisms. They are hypotheses, not definitive facts.
Phylogenetic tree
33
In a phylogenetic tree, more closely related species are closer or farther away from each other?
Closer
34
Is *Chlamydia* more closely related to *Escherichia* or *Clostridium*?
*Clostridium*
35
How are most phylogenetic trees constructed via modern technology?
Genome sequencing
36
\_\_\_\_\_\_\_\_\_\_ is most often found in the human mouth; it is the primary colonizer of teeth, creating a biofilm that recruits more bacteria and ultimately leads to cavities.
*Streptococcus*
37
What is "next generation" or "next gen" sequencing?
Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies. These technologies allow for sequencing of DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing, and as such revolutionised the study of genomics and molecular biology
38
Different areas of the body have different microbiomes, and it is believed that disruptions to these microbiomes can lead to \_\_\_\_\_\_\_\_\_\_\_\_\_\_.
Illness
39
Do the microbes in the gut of a premature infant change radically from day to day?
Yes, the microbes in the gut of a premature infant change radically from day 10 to days 16 through 21
40
Prokaryotes inhabit a wide range of habitats. In what temperature and pH ranges can they be found?
Temperature range: -10 degrees to 120 degrees celsius pH range: less than 1 to more than 10
41
What probably defines the limits of life based on the kind of chemistry that characterizes life on earth?
The range in which prokaryotes can survive
42
What is one of the biggest - if not the biggest - driving force for diversity among the microbes?
The immense range of the habitats in which they reside; ultimately this has resulted in evolutionary changes where prokaryotes have genes that lead to their ability to live in such environments
43
Would removing all plants and animals destroy life on earth?
No; although the ecosystem would be affected, microbes are sufficient to maintain a sustainable global ecosystem as they did for several billion years
44
Why are prokaryotes essential to *all* life on earth?
Because some catalyze transformations of chemical compounds essential to the sustainability of life
45
What two specific reactions can prokaryotes accomplish that most eukaryotes are unable to do?
Fixation of inorganic nitrogen into organic nitrogen and denitrification of nitrate (organic nitrogen) into nitrogen gas (inorganic nitrogen)
46
Briefly explain the nitrogen cycle.
47
\_\_\_\_\_\_\_\_\_\_\_\_ ____________ is the process in which N2 in the atmosphere breaks and combines with other compounds to form organic molecules.
Nitrogen fixation
48
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ is the process in which organic nitrogen (e.g., nitrate) is broken down into nitrogen gas.
Denitrification
49
Like all cells, microbes are mostly \_\_\_\_\_\_\_\_\_\_\_\_.
Water
50
Approximately how much water makes up microbes?
70%
51
The dry weight of microbial cells consists mainly of _______________________ and \_\_\_\_\_\_\_\_\_\_\_\_\_\_.
Macromolecules Lipids
52
What are macromolecules?
They are very large molecules with molecular weights above 10,000 Daltons with a polymeric structure
53
What are lipids?
Lipids are smaller molecules with molecular weights under 1,000 Daltons; they are *not* polymeric in structure, and they are highly hydrophobic
54
What are the three macromolecules?
Carbohydrates Proteins Nucleic Acids
55
Macromolecules constitue more than \_\_\_\_% of the dry weight of microbial cells.
85
56
Lipids constitute about \_\_\_% of the dry weiight of microbial cells.
10%
57
Among the macromolecules, _________________ are the largest and most diiverse category.
Proteins
58
There is something wrong with this figure. What is it?
The composition of a microbial cell is 70% water and 30% dry weight
59
According to the textbook, there are four major macromolecule categories. What are they?
Proteins Nucleic acids Polysaccharides Heteropolymers
60
What are heteropolymers?
Macromolecules in which two different types of monomers are covalently combined
61
What is the principle heteropolymer in *E. coli*?
Murein
62
What is murein?
A heteropolymeric structure found in the cell wall of bacteria like *E. coli* that is composed of many short peptides and a polysaccharide core
63
Another complex macromolecule or heteropolymer found in the cell walls of *E. coli* combines a polysaccharide with a lipid to form a \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_.
Lipopolysaccharide
64
The cytoplasm is a dense suspension of \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_.
Ribosomes
65
Why are there so many ribosomes in the prokaryotic cell?
1. Because about 54% of the dry mass of the cell is protein 2. Protein synthesis is slower than that of other macromolecules
66
Approximately how many ribosomes are present in the average *E. coli* cell?
18,000
67
The average *E. coli* cell has approximately ___ million proteins.
2 million
68
What are most of the small molecules of a microbial cell?
Intermediates in biochemical pathways or monomeric precursors of macromolecules
69
Why are small molecules so abundant if they make up only 5% of the dry weight of microbial cells?
Because they are so much smaller than the macromolecules, a very small mass of them consists of a very large number of molecules
70