Chapter 1 Exam 1 Flashcards
Independent variable
Manipulated in the experiment
Different types of mold
(Types of tomatoes)
Dependent variable
Measured variable. Provides quantitative data for analysis.
Taste, smell, “outcome”
Controlled
Maintaining identical conditions for all other variables between test groups
Alexander Fleming*
1928 Staphylococcus aureus Mold Penicillium notatum Hypothesis: mold is secreting something that kills bacteria
Positive control
Used to validate experimental procedure, to provide a positive result
Negative control
Used to validate experimental procedure, provides a negative result, shows the materials are not contaminated
Microorganisms
Too small to see wi the naked eye
Bacteria, archaea, algae, Protozoa, fungi (molds and yeast) viruses
Phylogeny
The study of evolutionary relationships among organisms
Genetic relatedness
Scientific method
Observation, hypothesis, experimentation, analysis, conclusion
Pathogenic
Disease producing
Carolus Linnaeus
1735 system of nomenclature
Genus (1st name) and species, italicized or underlined,
After a scientific name has been mentioned once, it can be abbreviated with the initial of the genus followed by the species name
Bacteria and archaea
Unicellular organisms
Genetic material not enclosed in a nuclear membrane (prokaryote)
Strepto
Chains
Enterica
Intestines
Sarccharomyces
Sugar fungus
Pyogen
Pus
Cerevisa
Beer
Chrysogenum
Yellow
Trypanosome
Borer body
Bacillus
Rod like
Coccus
Spherical or ovoid
Peptidoglycan
Protein carbohydrate cell wall
Binary fission
Reproducing by dividing into 2 equal parts
Archae
No Peptidoglycan
No human disease
Methanogens
Archaea that produce methane
Extreme thermophiles
Archaea that live in hot sulfurous water
Extreme halophiles
Archaea that live in extreme salt
Bacillus
Rod like
Coccus
Spherical or ovoid
Peptidoglycan
Protein carbohydrate cell wall
Binary fission
Reproducing by dividing into 2 equal parts
Archae
No Peptidoglycan
No human disease
Discovered by Charles woese
Methanogens
Archaea that produce methane
Extreme thermophiles
Archaea that live in hot sulfurous water
Extreme halophiles
Archaea that live in extreme salt
Eukaryotes (distinct nucleus containing the cell's Dna) Kingdom fungi Unicellular or multicellular No photosynthesis Chitin Yeast mold slime mold (amoeba + mold) Can reproduce sexually or asexually Can absorb organic material from the environment
Fungi
Yeasts
Oval
Larger than bacteria
Molds
Made of mycelia (composed of filaments called hyphae)
Cottony
Unicellular eukaryotes
Pseudopods, flagella or cilia
Free entities or parasites
Can reproduce sexually or asexually
Protozoa
Parasites
Derive nutrients from living hosts or ingest organic compounds from their environment
Euglena
Photosynthetic Protozoa
Photosynthetic eukaryotes Sexual and asexual reproduction Usually unicellular Cell walls of cellulose In Water soil or plants Do not generally require organic compounds from the environment Autotrophs - carbon fixation
Algae
Can only be seen with an electron microscope
Particle
Either Dna or rna
Can’t reproduce as self sufficient units using only cellular machinery of other organisms
Are not considered living because they are inert outside of living hosts
Viruses
Eukaryotes
Parasitic worms - flatworms and round worms
Helminths
Microscopic at some stage
Multicellular animal parasites
1978
Devised a system of classification for organisms of three domains (bacteria archaea eukaryotes)
Carl woese
Cell walls consisting of Peptidoglycan Prokaryote Fission do conjugation Bacilli, Coccus, or spiral Cilia or flagella Distinguished by habitat, biochemistry or DNA sequence
Bacteria
Cell walls lack Peptidoglycan
Archaea
Protists
Fungi
Plants
and animals
Eukarya
1665
Little boxes
Cell theory - all living things are made of cells
Robert Hooke*
Observed live microorganisms
Between 1673 and 1723 wrote about animalcules (bacteria and Protozoa)
Anton van Leeuwenhoek*
1668
Demonstrated that flies did not come from decaying meat (spontaneous generation)
Jars
Air got through mesh screens
Francesco redi*
Life could emerge spontaneously from no living matter
Spontaneous generation
Spontaneous generation theory strengthened 1745 Mutton broth Heated solutions used unsterile corks "Vital force"
John needham*
Microorganisms from the air
Countering needham
Conclusions: air transported germs
Lazzaro spallazani*
Microorganisms are present in the air and can contaminate sterile solutions but air does not create microorganisms
Curved neck flasks
Filtered Microorganisms Out of the air with cotton
Broth did not decay because the curved neck trapped airborne microorganisms
Broke necks of flask to open air (contamination)
Also demonstrated that microorganisms can be destroyed by heat (Pasteurization)
Beginning of aseptic technique
Fowl cholera loses it’s virulence over time
Coined term vaccine
Louis pasteur*
1857 thru 1914
Golden age of microbiology
Studied chemical activities of Microorganisms Techniques for microscopy Culturing organisms Development of vaccines Surgical techniques
Microorganisms cause disease
Germ theory of disease
English surgeon
Semmelweis
Childbirth
Phenol kills bacteria
Joseph lister
Proof disease came from bacteria
1876
Anthrax
Robert Koch
Koch’s postulates
Cowpox/smallpox
8 yr old volunteer
Edward Jenner
Protection from a disease from a vaccine or recovery from the disease itself
Immunity
Produced from antivirulent microbial strains or killed microbe
Vaccine
Treatment of disease using chemical substances
Chemotherapy
Chemicals produced by bacteria or fungus to act against other microorganisms
Antibiotics
Chemotherapeutic agents prepared from chemicals in the laboratory
Synthetic drugs
Magic bullet hunts down pathogens without destroying the host
Paul erlich
Penicillium Chrysogenum
Not until the 1940’s
Alexander Fleming
Staphylococcus aureus and enterococcus feacalis
Vancomycin resistant
Scientific process
Observation hypothesis experimentation analysis conclusion
Grouping organisms according to their similarities
Taxonomy
Made of rna and protein
Ribosomes
Bacilli, cocci, spirilla
Rods, spheres, spirals
Strep (chain)
Staph (clusters)
Palisade
Arrangements
Diplococci
Tetrads
Sarcinae
Pairs of cocci
Packets of 4
Packets of 8, 16, 32 cells
Neisseriae
Bean shaped In Pairs
Coccobacilli
Chains?
Vibrios
Curved rods
Streptococci
Cocci in chains
Streptomyces
Mold like fillimentus bacteria
Spirochetes
Syphalis
Prokaryotes are simpler organisms
Mitochondria and chloroplasts have attributes of living organisms
These organelles have their own dna and are enclosed in a double membrane
These organelles may have been engulfed by a larger cell
(Essay question!)
Endosymbiotic theory
Schwann and schleiden*
Found that all plants and animals were made up of cells
All life arises from previous life
Biogenesis
The study of fungus
Mycology
The study of Protozoa and parasitic worms
Parasitology
Study of an organisms genes
Genomics
Tobacco mosaic virus
Dmitri iwanowski
Fragments of human or animal dna can be attached to bacterial dna resulting In a hybrid
Recombinant dna
Recombinant dna (rdna) technology
Inserts recombinant Dna into bacteria to make large quantities of a desired protein
How genetic information is carried in molecules of dna and how dna directs the synthesis of proteins
Molecular biology
Bacteria are less complex
They can be cultivated In large #s
In a short time
Why use bacteria in research
The study of the relationship between Microorganisms and the enevironment
Microbial ecology
Commercial use of Microorganisms to produce some common foods and chemicals
Biotechnology
Inserting a missing gene or replacing a defective gene in human cells
Gene therapy
Variety of Microorganisms on and Inside our bodies
Normal microbiota or flora
Ability to ward off disease
Interferons (antimicrobial chemicals)
Resistance
Complex aggregation of microbes
Protect your mucus membranes from harmful microbes
Often resistant to antibiotics
Biofilm
Disease in which a pathogen invades a susceptible host, carries at lease the part of it’s life cycle Inside the host
Infectious disease
EIDs
Emerging Infectious Diseases
MERS
Middle eastern respiratory syndrome
SARS
Severe acute respiratory syndrome
H1N1
Swine flu (influenza)
H5N1
Avian influenza
MRSA
Methicillin resistant staphylococcus aureus
VISA
Vancomycin resistant staphylococcus aureus
BSE
Bovine spongiform encephalopathy
Mad cow
Like Creutzfeldt-Jakob disease (human)
Infectious protein
Prion
E.coli O157:H7
Bloody diarrhea
Undercooked meat
EHF
Ebola hemorrhagic fever
75% die
Marberg virus
Another hemorrhagic fever
Cryptosporidiosis
Public water supply
Milwaukee
Diarrheal illness
What is the identity of this mold?
What other organisms are being affected by this mold?
What is the mold doing to kill the bacteria?
Alexander Fleming’s experiments
Grouping organisms according to similarities
Taxonomy
Discovered by Carl woese Lack Peptidoglycan Thermophiles Halophiles Methanogens
Archeae
Unicellular
Absorb nutrients or engage in parasitism
Motile
Pseudopods (amoeba)
Protozoa
Photosynthetic
Autotrophs (carbon fixation)
Algae
Found that plants ad animals were made up of individual cells
Schwann and schleiden*
All life arises from previous life
Biogenesis