Chap 20 Bio. Tech

Question 0

Genetic engineering

Answer 0

The science of altering and cloning DNA to make substances

Question 1

The use of microorganismsto make commercial products

Answer 1

Biotechnology

Question 2

Recombine DNA

Answer 2

DNA from two or more sources that are put into a single molecule

Question 3

Restriction enzymes

Answer 3

Bacterial enzymes that cut DNA at specific base pairs

Question 4

Why do bacteria have restriction endonuleases

Answer 4

To cut out foreign DNA after assimilation of external DNA occurs

Question 5

Vectors

Answer 5

Self replicating DNA molecules used to transfer foreign DNA segments between hosts

Question 6

Examples of expression vectors

Answer 6

Plasmids, yeast, animal virus gnome, phages

Question 7

Science

Answer 7

The search for knowledge about ourselves and the physical world

Question 8

First step of DNA isolation

Answer 8

Disrupt cells membrane

Question 9

Step 2 of DNA isolation

Answer 9

Precipitate DNA with ethanol

Question 10

Step 3 of DNA isolation

Answer 10

Retrieve DNA precipitate and store

Question 11

Step 4 of DNA isolation

Answer 11

dissolve in water and repeat steps

Question 12

PCR stands for

Answer 12

Polymerase chain reaction

Question 13

Materials for PCR

Answer 13

DNA polymerase
Isolated DNA
Nucleotides
Synthetic primers

Question 14

Steps of PCR

Answer 14

Denature DNA (95C) 
Primer annealing (50-65C)
DNA stand synth. (72C)

Question 15

Collection of molecules or cells all identical to the original organism or cell

Answer 15

Clone

Question 16

Benefits of using microbes

Answer 16

Grow fast
Small
Economical
Easy to isolate genes

Question 17

Sticky ends

Answer 17

DNA stands with extra affinity to each other

Question 18

Gel electrophoresis

Answer 18

Separates molecules based on size and charge

Question 19

Electrophoresis materials

Answer 19

Agros 
Buffer
DNA ladder 
Power supply 
Ethidium bromide ( fluorescent chemical )

Question 20

Membrane matrix

Answer 20

Nylon or nitrocellulose

Question 21

Types of sequencing

Answer 21

1 radioactive labeled DNA molecules

2 chain termination method