chap 14 Flashcards
wet mount and hanging drop preparation
motility test
stainings are
gram staining- acid fast staining- structural staining
comsist of plastic strips and microtubes that contain dehydrated biochemical substrates
analytical profile index
commony used for identification of gram negative enteric bacteria
analytical profile index
analytical profile index is incubated for
18-24 hours at 35 degree celsius
in this method known pattern of microbial growth are compared with test organisim using computer software
automated method for identification of bacteria
pure bacterial isolate during incubation at
35 DC for 16-24 hours
producing acid or alkaline end products as early as
2 hours
a basic detection of antibody response from antigenic simulatuon
serological test
one diagnosic test for organism that are difficult to culture - atypical bacteria
serological test
performed by adding antibodies to bacter suspension thay will bind to surface of organism
bacterial agglutination
used to identify bacteria that are difficult to cultivate in culture medium
give ex.
positive results
bacterial agglutination
widal test
visible clumps/agglutination
uses artificial carriers such as katex particles or treated RBC or biological carrier like bacterial cells as reagent which can adsorb test antigen and react with specific antibody
particle agglutinations
in this test, antigen in patient specimens bind to antibodies that present in surface of latex beads
latex agglutination
uses antubody bound to particle to increase visibility or agglutination reactions btween antigen and antibody
coagglutination
composed of two parts that test systems include antigen causes the diseases and indicator which consist sheeps red blood cells, complement fixing antibody
complement fixation
uses soluble antigen that reacting with antibodies in serum sample
flocculation tests
sensitive and specific method for detection of antibodies against certain pathogens
ELISA enzyme linked immunosorbent ASSAY
commonly used for rapid identification of bacterial and viral antigens
immunofluorescent assay
utilizef fluorescent dye and specific kabeled antibodies
direct FAT
two step or sndwich tech thaht is mor sensitive than other test of FA
Indirect FAT
based on electrophoretic separation of bacterial protein in supporting media
western blot
strain typing tech that useful addition to epidemiologic research
pulsed field gel electrophoresis
virus that attack’s bacteria
bacteriophage
this method is based on specificity of phage surface receptors for cell
bacteriophage typing
currently considered most important method for microbial identification
molecular diagnosis
most commonly used amplification tech in molecular diagnosis
polymerase chain reaction
a dye that binds to double stranded DNA but not tonsingle stranded
SYBR green
determine the order kf nucleotides in DNA fragments
DNA sequencing
molecular approach that does not necessiate nuclear acid amplification
has h specificity and h sensitivity
fluorescence in situ hybridization
refers to grouping of DNA at micro or nano level
microarrays and nanoarrays
detect gene expression of target microorganisms, identifies bacte, new gene and mutation
microarrays and nanoarrays
It is the study ofmicrobialgenes collectedfrom the environment.
metagenomics
is useful in the study of the indigenous flora in the human body and gut
metagenomics
Itis the study of proteins (proteome) on cellular level.
proteomics
In clinical microbiology, it identifies protein expression i n biosamples suspected with pathogens.
It also determines the effectiveness of drug therapy across diseases.
proteomics
