chap 11 part 2 Flashcards
utilized for the isolation of aerobic and anaerobic bacteria,
microaerophiles and fungi.
blood culture bottle
added into the medium to absorb any antibiotics that have been taken by the individual and are present in the blood at thetime of
fica • samplecollection.
antimicrobial removal device or resins
manual blood culture system
septi chek
oxoid signal -microaerophile
lysis centrifugation - fastidious, mycobacteria and fungi
automated blood culture system
BACTEC SYSTEM
After plating or transferringthe mediato tubes,a representativeuninoculatedsampleof each ir batch of preparation should be incubated overnight at___ to observe for the presence of
contaminants (bacterial growth).
35°C
Some examples of specimens that require a direct or “bedside”inoculation are ___ and
___for isolation of Bordetella pertussis.
blood, genital specimens, corneal scrapings, sterile fluids like synovial and peritoneal fluids,
nasopharyngeal swabs
most common manner of inoculation
streaking
A specimen that is collected through a swab is inoculated by gently ___ onto the upper edgeoftheplate,thentheinoculatedareashouldbe streaked _____ by a sterile loop to distribute the specimen.
“rolling the tip of the swab”
(quadrant streaking technique)
The ___ of the medium is usually performed with group A streptococci to create anaerobiosis and promotesub-surface hemolysis.
“stabbing”
isusedforantimicrobialsusceptibilitytest (
Overlappinginoculation
Urine specimens are inoculated using a quantitative isolationtechnique with a calibrated loop 9125 (_____)todeliveraspecifiedvolume.
0.01mLor0.001mL
more susceptible to contamination
specimen collected at bedside
W hen m o re than one agar plate is used, inoculation should start from the __
nonselective to the selective agar plates.
two types of anaerobic chamber system
sealed glove box and gloveless chamber
serve as inoculation hood and incubator
anaerobic chamber
acts as a filler for the remaining percentage of the anaerobic atmosphere
nitrogen gas
facilitate the growth and isolation of anaerobes
hydrogen and carbon dioxide gases
remove residualoxygen from the chamber by combining with
hydrogen to form water
palladium catalyst pellets
absorbsthe water that is formed when hydrogen combines with the free
oxygen
silica gel (dessicant)
n oxygen-reduction indicator that becomes colorless in the absence
of oxygen
methylene blue/reazurin
When the CO,content of ananaerobic incubatorisincreasedto _%, the oxygen islowered to approximately __
10%,
18%.
requires laboratories to conduct daily monitoring of the anaerobic chamberusing eitherof the oxygen reductionindicatorsto determine if the
incubator achieves the desired purpose.
College of American Pathologist (CAP)
ideal anaerobic incubation system
anaerobic chamber
has the same components of anaerobic chamber except that gases are in envelopes pouch
gas pak jar
For most bacteria, the idealincubation temperature is __
35°C to 37•C
aerobic culture should be examined __
after 18 hour of inoculation
anaerobic culture require ___ of incubation
24-48hrs of incubation
Most routine bacterial cultures whether in broth or solid media are held for ___
48 hours to 72 hours.
Cultures for anaerobes may be held for ___
5 (automated) days to 7 days.(manual)
Slow growing bacteria, such asmycobacteria,require ___ of continuous incubation under the desired conditions before visible colonies appear.
14 days to m o re than a month
____ mayrequire specialmedia oratmosphere beyondtheroutine setup.
Atypical bacteria
