chap 11 part 2

Question 1

utilized for the isolation of aerobic and anaerobic bacteria,
microaerophiles and fungi.

Answer 1

blood culture bottle

Question 2

added into the medium to absorb any antibiotics that have been taken by the individual and are present in the blood at thetime of
fica • samplecollection.

Answer 2

antimicrobial removal device or resins

Question 3

manual blood culture system

Answer 3

septi chek
oxoid signal -microaerophile
lysis centrifugation - fastidious, mycobacteria and fungi

Question 4

automated blood culture system

Answer 4

BACTEC SYSTEM

Question 5

After plating or transferringthe mediato tubes,a representativeuninoculatedsampleof each ir batch of preparation should be incubated overnight at___ to observe for the presence of
contaminants (bacterial growth).

Answer 5

35°C

Question 6

Some examples of specimens that require a direct or “bedside”inoculation are ___ and
___for isolation of Bordetella pertussis.

Answer 6

blood, genital specimens, corneal scrapings, sterile fluids like synovial and peritoneal fluids,

nasopharyngeal swabs

Question 7

most common manner of inoculation

Answer 7

streaking

Question 8

A specimen that is collected through a swab is inoculated by gently ___ onto the upper edgeoftheplate,thentheinoculatedareashouldbe streaked _____ by a sterile loop to distribute the specimen.

Answer 8

“rolling the tip of the swab”

(quadrant streaking technique)

Question 9

The ___ of the medium is usually performed with group A streptococci to create anaerobiosis and promotesub-surface hemolysis.

Answer 9

“stabbing”

Question 10

isusedforantimicrobialsusceptibilitytest (

Answer 10

Overlappinginoculation

Question 11

Urine specimens are inoculated using a quantitative isolationtechnique with a calibrated loop 9125 (_____)todeliveraspecifiedvolume.

Answer 11

0.01mLor0.001mL

Question 12

more susceptible to contamination

Answer 12

specimen collected at bedside

Question 13

W hen m o re than one agar plate is used, inoculation should start from the __

Answer 13

nonselective to the selective agar plates.

Question 14

two types of anaerobic chamber system

Answer 14

sealed glove box and gloveless chamber

Question 15

serve as inoculation hood and incubator

Answer 15

anaerobic chamber

Question 16

acts as a filler for the remaining percentage of the anaerobic atmosphere

Answer 16

nitrogen gas

Question 17

facilitate the growth and isolation of anaerobes

Answer 17

hydrogen and carbon dioxide gases

Question 18

remove residualoxygen from the chamber by combining with
hydrogen to form water

Answer 18

palladium catalyst pellets

Question 19

absorbsthe water that is formed when hydrogen combines with the free
oxygen

Answer 19

silica gel (dessicant)

Question 20

n oxygen-reduction indicator that becomes colorless in the absence
of oxygen

Answer 20

methylene blue/reazurin

Question 21

When the CO,content of ananaerobic incubatorisincreasedto _%, the oxygen islowered to approximately __

Answer 21

10%,

18%.

Question 22

requires laboratories to conduct daily monitoring of the anaerobic chamberusing eitherof the oxygen reductionindicatorsto determine if the
incubator achieves the desired purpose.

Answer 22

College of American Pathologist (CAP)

Question 23

ideal anaerobic incubation system

Answer 23

anaerobic chamber

Question 24

has the same components of anaerobic chamber except that gases are in envelopes pouch

Answer 24

gas pak jar

Question 25

For most bacteria, the idealincubation temperature is __

Answer 25

35°C to 37•C

Question 26

aerobic culture should be examined __

Answer 26

after 18 hour of inoculation

Question 27

anaerobic culture require ___ of incubation

Answer 27

24-48hrs of incubation

Question 28

Most routine bacterial cultures whether in broth or solid media are held for ___

Answer 28

48 hours to 72 hours.

Question 29

Cultures for anaerobes may be held for ___

Answer 29

5 (automated) days to 7 days.(manual)

Question 30

Slow growing bacteria, such asmycobacteria,require ___ of continuous incubation under the desired conditions before visible colonies appear.

Answer 30

14 days to m o re than a month

Question 31

____ mayrequire specialmedia oratmosphere beyondtheroutine setup.

Answer 31

Atypical bacteria