chap 11 Flashcards
are the growth of microorganism in a culture medium
culture
culture medium is composed of mixture of nutrients such as
carbon, nitrogen, sulfur, phosphorus, hydrogen, oxygen, and buffer
patterns of microbial growth
liquid, semi-solid, solid medium
these inhibitory agents are added into medium to facilitate the isolation of desired bacteria while suppressing other microorganism presents in the sample
dyes, salt, and antimicrobials
if several plates will be inoculated, media should be arranged from__ to __
most enriched to selective
isolation/identification of microorganism in the areas of industrial and food microbiology requires
specialized culture media
types of culture
pure
mixed
stock culture
contains a single species
pure culture
more than one species
mixed culture
composed of several species contained in a separate culture medium
stock culture
should be cultivated in a large volume of broth and divided small vials to lengthens the shelf life of culture for a year
stock culture
used for academic and industrial purposes
stock culture
classification of culture media
according to consistency
liquid medium
semi-solid medium
solid medium
does not contain any amount of agar
liquid medium
liquid medium allow the growth of
aerobes, anaerobes, and facultative anaerobes
ex; of liquid medium
brain heart infusion
trypticase soy broth
thioglycollate
semi solid medium contain ___ agar
0.5 % to 1% agar
used to observed bacterial motility and detect indole and sulfide production
semi solid medium
ex semi solid
sulfide indole motility medium
solid medium contains __ agar
2% to 3% agar
ex. of solid medium
triple sugar iron agar
macconkey agar
blood agar plate
chocolate agar plate
a sulfated polymer
agar
agar is made up of
d-galactose
3,6-anhydro-L-galactose
D glucuronic acid
agar is usually derived from
red algae
cooling temp of culture medium to petrinplates is
55-60 degree celsius
melting temp of agar __
80-90 degree celsius
agar solidify at
40-50 degree celsius
molten agar in amount of ___ should be transferred to __
20-25 mL
STERILEPLATES
according to composition
synthetic or defined medium
non synthetic or complex medium
tissue culture medium
medium which all components are known
synthetic or defined medium
designed for research purposes either liquid or solid medium
synthetic or defined medium
preferred for the isolation of cyanobacteria and chemoorganotrops
synthetic or defined medium
synthetic or defined medium is preferred for isolation of
cyanobacteria and chemoorganotrophs
synthetic or defined medium ex
BG-11 medium
medium which some of substance are unknown
non synthetic or complex medium
(peptone, yeast extract, and meat)
useful for isolation of medically significant bacteria
non synthetic or complex medium
non synthetic or complex medium ex
nutrient broth medium
trypticase soy broth
macconkey agar
utilized for obligate intracellular bacteria
tissue culture medium
(rickettsia and chlamydia)
ex tissue culture medium
all of these ex are used for isolation of
heLa 229 cells- human cervical tissue cells
mcCoy cells and w 138 cells - fibroblast
chlamydia
utilized for propagation of rickettsia
embryonated eggs
plated media are distributed into __
dish or plate
tube media prepared either
liquid, slant, buut, and slant or butt
according to dispensing what are the examples
triple sugar iron agar
sulfide indole motility medium
simmon’s citrate agar
lysine iron agar
routine in laboratory without additional supplements
supportive media/simple media
media that support most non fastidious bacteria
supportive media/simple media
supportive media/simple media is usually composed of
meat and soybean extracts
ex supportive media/simple media
nutrient agar
trypticase soy broth
nutrient broth
propagate certain group of bacteria from mixture of organism
enrichment mediacon
contain specific nutrients and without additional supplements
enrichment media
these media are incubated for certain periods then subculture to isolate desired organism
enrichment media
used as supplement to agar plate to detect aerobes, anaerobes, microaerophiles
enrichment media
enrichment media ex.
gram negative broth
alkaline peptone water
alkaline peptone water adjusted to ph__ which is best for ___ before inoculation into __
8.4
vibrio spp.
thiosulfate citrate bile-salt-sucrose agar
selenite F is for isolation of __
salmonella
__ is for isolation of salmonella
selenite f
general support enrichment medium that promotes growth of almost all non-fastidious bacteria
thioglycollate
enrichment broth for salmonella and proteus
tetrathionate
tetrathionate is for isolation of
salmonella and proteus
it is both enrichment and selective medium that contains sodium citrate and sodium deoxycholate
gram negative broth w/c inhibit gram positive bacteria
enrichment for group B streptococci
lim broth
lim broth is for isolation of
group B streptococci
media with additional supplements such as blood, vit, yeast extract for growth of fastidious organism and ex.
enriched media and non selective media
BAP (BLOOD AGAR PLATES) AND CAP (COLUMBIA BLOOD AGAR
solid type of media
enriched media and non selective media
utilized to differentiate hemolytic patterns of bacteria
BAP (BLOOD AGAR PLATES)
used for recovery of haemophilus spp.
columbia blood agar
promote isolation of fastidious organism
blood agar plates
columbia blood agar
this media allows visualization of metabolic differences between groups of media
differential media
ph indicator of macconkey agar
neutral red
__ separates lactose fermenters ___ from non lactose fermenters ___
macconkey agar
pink colonies
colorless colonies
___ Characterized bacteria and recommended for urine culture
cystine lactose electrolyte deficient agar
yellow colonies
media incorporated with antibiotics, dyes/chemicals to inhibit growth of other organisms while promoting growth of desired bacteria
ex
selective media
MAC
to inhibit gram positive bacteria
crystal/ gentian violet,basic/carbon fuchsin and bile salts
to inhibit gram negative bacteria
potassium tellurite and sodium azide
to inhibit swarming of swarming bacteria
alcohol and chloral hydrate
used to isolate bacteria with specific growth requirement
ex.
special media
thiosulfate citrate bile salt sucrose agar
lowenstein jensen medium
composed of whole eggs and malachite green
lowenstein jensen medium
supports growth through inspissation and not autoclaving
lowenstein jensen medium
selective for sterilization through boiling and not autoclaving
thiosulfate citrate bile salt sucrose agar
for vibrio spp.
