Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

bacte > chap 11 > Flashcards

chap 11 Flashcards

1
Q

are the growth of microorganism in a culture medium

A

culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

culture medium is composed of mixture of nutrients such as

A

carbon, nitrogen, sulfur, phosphorus, hydrogen, oxygen, and buffer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

patterns of microbial growth

A

liquid, semi-solid, solid medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

these inhibitory agents are added into medium to facilitate the isolation of desired bacteria while suppressing other microorganism presents in the sample

A

dyes, salt, and antimicrobials

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

if several plates will be inoculated, media should be arranged from__ to __

A

most enriched to selective

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

isolation/identification of microorganism in the areas of industrial and food microbiology requires

A

specialized culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

types of culture

A

pure
mixed
stock culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

contains a single species

A

pure culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

more than one species

A

mixed culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

composed of several species contained in a separate culture medium

A

stock culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

should be cultivated in a large volume of broth and divided small vials to lengthens the shelf life of culture for a year

A

stock culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

used for academic and industrial purposes

A

stock culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

classification of culture media
according to consistency

A

liquid medium
semi-solid medium
solid medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

does not contain any amount of agar

A

liquid medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

liquid medium allow the growth of

A

aerobes, anaerobes, and facultative anaerobes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

ex; of liquid medium

A

brain heart infusion
trypticase soy broth
thioglycollate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

semi solid medium contain ___ agar

A

0.5 % to 1% agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

used to observed bacterial motility and detect indole and sulfide production

A

semi solid medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

ex semi solid

A

sulfide indole motility medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

solid medium contains __ agar

A

2% to 3% agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

ex. of solid medium

A

triple sugar iron agar
macconkey agar
blood agar plate
chocolate agar plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

a sulfated polymer

A

agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

agar is made up of

A

d-galactose
3,6-anhydro-L-galactose
D glucuronic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

agar is usually derived from

A

red algae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

cooling temp of culture medium to petrinplates is

A

55-60 degree celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

melting temp of agar __

A

80-90 degree celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

agar solidify at

A

40-50 degree celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

molten agar in amount of ___ should be transferred to __

A

20-25 mL
STERILEPLATES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

according to composition

A

synthetic or defined medium
non synthetic or complex medium
tissue culture medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

medium which all components are known

A

synthetic or defined medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

designed for research purposes either liquid or solid medium

A

synthetic or defined medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

preferred for the isolation of cyanobacteria and chemoorganotrops

A

synthetic or defined medium

32
Q

synthetic or defined medium is preferred for isolation of

A

cyanobacteria and chemoorganotrophs

33
Q

synthetic or defined medium ex

A

BG-11 medium

34
Q

medium which some of substance are unknown

A

non synthetic or complex medium
(peptone, yeast extract, and meat)

35
Q

useful for isolation of medically significant bacteria

A

non synthetic or complex medium

36
Q

non synthetic or complex medium ex

A

nutrient broth medium
trypticase soy broth
macconkey agar

37
Q

utilized for obligate intracellular bacteria

A

tissue culture medium
(rickettsia and chlamydia)

38
Q

ex tissue culture medium
all of these ex are used for isolation of

A

heLa 229 cells- human cervical tissue cells
mcCoy cells and w 138 cells - fibroblast
chlamydia

39
Q

utilized for propagation of rickettsia

A

embryonated eggs

40
Q

plated media are distributed into __

A

dish or plate

41
Q

tube media prepared either

A

liquid, slant, buut, and slant or butt

42
Q

according to dispensing what are the examples

A

triple sugar iron agar
sulfide indole motility medium
simmon’s citrate agar
lysine iron agar

43
Q

routine in laboratory without additional supplements

A

supportive media/simple media

44
Q

media that support most non fastidious bacteria

A

supportive media/simple media

45
Q

supportive media/simple media is usually composed of

A

meat and soybean extracts

46
Q

ex supportive media/simple media

A

nutrient agar
trypticase soy broth
nutrient broth

47
Q

propagate certain group of bacteria from mixture of organism

A

enrichment mediacon

48
Q

contain specific nutrients and without additional supplements

A

enrichment media

49
Q

these media are incubated for certain periods then subculture to isolate desired organism

A

enrichment media

50
Q

used as supplement to agar plate to detect aerobes, anaerobes, microaerophiles

A

enrichment media

51
Q

enrichment media ex.

A

gram negative broth
alkaline peptone water

52
Q

alkaline peptone water adjusted to ph__ which is best for ___ before inoculation into __

A

8.4
vibrio spp.
thiosulfate citrate bile-salt-sucrose agar

53
Q

selenite F is for isolation of __

A

salmonella

54
Q

__ is for isolation of salmonella

A

selenite f

55
Q

general support enrichment medium that promotes growth of almost all non-fastidious bacteria

A

thioglycollate

56
Q

enrichment broth for salmonella and proteus

A

tetrathionate

57
Q

tetrathionate is for isolation of

A

salmonella and proteus

58
Q

it is both enrichment and selective medium that contains sodium citrate and sodium deoxycholate

A

gram negative broth w/c inhibit gram positive bacteria

59
Q

enrichment for group B streptococci

A

lim broth

60
Q

lim broth is for isolation of

A

group B streptococci

61
Q

media with additional supplements such as blood, vit, yeast extract for growth of fastidious organism and ex.

A

enriched media and non selective media
BAP (BLOOD AGAR PLATES) AND CAP (COLUMBIA BLOOD AGAR

62
Q

solid type of media

A

enriched media and non selective media

63
Q

utilized to differentiate hemolytic patterns of bacteria

A

BAP (BLOOD AGAR PLATES)

64
Q

used for recovery of haemophilus spp.

A

columbia blood agar

65
Q

promote isolation of fastidious organism

A

blood agar plates
columbia blood agar

66
Q

this media allows visualization of metabolic differences between groups of media

A

differential media

67
Q

ph indicator of macconkey agar

A

neutral red

67
Q

__ separates lactose fermenters ___ from non lactose fermenters ___

A

macconkey agar
pink colonies
colorless colonies

68
Q

___ Characterized bacteria and recommended for urine culture

A

cystine lactose electrolyte deficient agar
yellow colonies

69
Q

media incorporated with antibiotics, dyes/chemicals to inhibit growth of other organisms while promoting growth of desired bacteria
ex

A

selective media
MAC

70
Q

to inhibit gram positive bacteria

A

crystal/ gentian violet,basic/carbon fuchsin and bile salts

71
Q

to inhibit gram negative bacteria

A

potassium tellurite and sodium azide

72
Q

to inhibit swarming of swarming bacteria

A

alcohol and chloral hydrate

73
Q

used to isolate bacteria with specific growth requirement
ex.

A

special media
thiosulfate citrate bile salt sucrose agar
lowenstein jensen medium

74
Q

composed of whole eggs and malachite green

A

lowenstein jensen medium

75
Q

supports growth through inspissation and not autoclaving

A

lowenstein jensen medium

76
Q

selective for sterilization through boiling and not autoclaving

A

thiosulfate citrate bile salt sucrose agar
for vibrio spp.

77
Q
A

bacte (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions