chap 11 part 3 Flashcards
Coloniesrangefromsmall(pinpoint)tolargewhile others appear mucoid or slimy.
size
Margin of coloniesis mostly
smooth or entire
As to elevation, colonies mayappear ___
raised,flat, convex or sometimes umbonate.
Colonies may bepigmented or colored, butnotall bacterial species havethis distinctive
feature.
chromogenesis
Colonies may appear ___
optical feature
opaque, transluscent, shiny, opalent
Growth of bacteria may produce putrid, fruity, ammoniacal, or ___.
odor - aromatic odor
growthmay be scanty,moderate, orabundant
amount
colonies may appear butyrous o r butter-like, viscous or stingy, dry and brittle
consistency
colonies may be pigmented
chromogenesis
It is theperiodwhenthereis nocelldivision oran abruptincrease in the cellnumber.
lag phase or period of rejuvenescence
It is the start of biosynthesis although there is no increase in cell mass.
lag phase or period of rejuvenescence
It is the adjustmentphasetoanewenvironment
lag phase or period of rejuvenescence
Itis the period when microorganismsare actively growing anddividing.
log or exponential phase
I t i s t h e stage in which the bacteria increaselogarithmically sincecellular production is most
activeduringthis period.
log or exponential phase
microorganisms areutilized in physiological, biochemical. and antimicrobial testing
log or exponential phase
period when there is balance between cell division and dying organisms and viable microorganisms remains constant
stationary/plateau phase
phase where metabolic activity of surviving cells slow down and become limited
stationary/plateau phase
phase which dead debris starts to accumulate
stationary/plateau phase
period when there is cessation of bacterial growth as no. of dead cells exceeds living microorganisms
decline phase
phase where there is loss of nutrients and increase toxic waste
death or decline phase
doubling of cell number
generation
time required for bacteria to double their population
doubling time/generation time
It is the most common asexualreproductive process in which a single cell dividesinto two daughter cells after developing a transverse cell wall.
transverse binary fission
growth measurement
by cell count, cell mass, cell activity
microscopy,useof electronicparticlecounter, or colonycount
cell count
weighing,measuringthenitrogencontent, andturbidimetry
cell mass
observationof thebiochemicalactivity
cell activity
measured volume of a bacterialsuspension is placed on a microscope slide or on a counting chamber.
Itdoesnotdistinguishbetweenlivingcells and dead cells
microscopic count
It is the most commonlyused method. It measures the number of: viable cells.
plate count
30-300 colonies
It utilizes a polycarbonate membrane filter or millipore filter.
membrane or molecular filter
requires 10 to 100 million cells/mL.
turbidimetric method
requires 10 to 100 million cells/mL.
turbidimetric method
