CH6-golgi Flashcards
cis face
entry
trans face
exit
Movement steps
1- vesicle move from ER to golgi
2- Vesicle come together to make new cis-Golgi cisternae
3-Maturation of cisternae towards trans direction
4-Vesicle form and leave golgi with proteins
5-Vesicle transport to golgicis cisternae
6-Vesicle transport protein back to ER
Function of golgi
make oligosaccharide
Movement of ER–> end vesicle thru golgi
CGN - P. oligosacch on lysosomal prot (sorting)
cis-cisternae - Remove Man
med-cisternae - Remove Man and add GlcNac
trans-cisternae - Add Gluc Add NANA
TGN - S. of tyr and carbohydrates (sorting
goes to lysosomes plasma membrane and secretoory vesicles
What type of transmembrane proteins are the glycosidases and glycosyl transferase
Single pass -Transmembrane proteins
What are the two classes of N-linked oligosacch
Complex and High mannose
N-link glyocsylation
Add to NH3- to ASN
O-link glycosylation
Add OH to ser/threo/OH-Lysine
What is the difference between vesicular transport model and cisternal maturation
IN v.tran golig is static , molecules move thru
in cisternal maturation, as the molecules pass, the cisternae matures .goes from cis-med-trans
What keeps the rab proteins close to the golgi
Golgins
From ER to golgi
1-Nucl envelope connects to RER and then that to SER
2- Golgi vesicles take the proteins from ER to golgi
3-Golgi takes the vesicles away and transfer to lysosomes/vacuoles
4-Lysosomes fuse for digestion
5-Transport vesicles are for
6-secretion into the plasma membrane
What are the 3 types of coats on vesicles
Clathrin
COP-I and COP-II
Where does calthrin coat transport from to
plasma membran to golgi/endosome
where does the COP-I go from
From cis-golgi to ER
Where does COP-II go from
From ER to cis-golgi
Assembly and dis-assembly of clathrin coat
Step1: PI - marking
when Ap2 binds to phospholipid it changes confirmation for it to bind to cargo-R
Step2-BAR-prot bends the membrane
Step3- cytoplasmic proteins (dynamin for ex: cuts the bud off)
Step4-GTP’ase ARF takes care of time and space of vesicle assembly
ARF
GTP’ase for clahtrin and COPI
Sar1
GTP’ase for COPII
Rab protein
surface markers that bind to Rab effectors
Tethering
Rab effector interact with Rab
SNARE prot pair
Docking vesicle to membrane
Rab disociate and return to cytosol once the vesicle is bound
How do viruses infect
Fusion
What brings material from ER to golgi
vesicular tubular clusters with COPII
Retrieval pathways
after clusters bud off, COP-I come in and carry back ER proteins that got away, and
What sequence is for ER membrane proteins
KKXX-retrieval sequence
What sequence is for soluble ER proteins
KDEL
