CH6-golgi Flashcards
cis face
entry
trans face
exit
Movement steps
1- vesicle move from ER to golgi
2- Vesicle come together to make new cis-Golgi cisternae
3-Maturation of cisternae towards trans direction
4-Vesicle form and leave golgi with proteins
5-Vesicle transport to golgicis cisternae
6-Vesicle transport protein back to ER
Function of golgi
make oligosaccharide
Movement of ER–> end vesicle thru golgi
CGN - P. oligosacch on lysosomal prot (sorting)
cis-cisternae - Remove Man
med-cisternae - Remove Man and add GlcNac
trans-cisternae - Add Gluc Add NANA
TGN - S. of tyr and carbohydrates (sorting
goes to lysosomes plasma membrane and secretoory vesicles
What type of transmembrane proteins are the glycosidases and glycosyl transferase
Single pass -Transmembrane proteins
What are the two classes of N-linked oligosacch
Complex and High mannose
N-link glyocsylation
Add to NH3- to ASN
O-link glycosylation
Add OH to ser/threo/OH-Lysine
What is the difference between vesicular transport model and cisternal maturation
IN v.tran golig is static , molecules move thru
in cisternal maturation, as the molecules pass, the cisternae matures .goes from cis-med-trans
What keeps the rab proteins close to the golgi
Golgins
From ER to golgi
1-Nucl envelope connects to RER and then that to SER
2- Golgi vesicles take the proteins from ER to golgi
3-Golgi takes the vesicles away and transfer to lysosomes/vacuoles
4-Lysosomes fuse for digestion
5-Transport vesicles are for
6-secretion into the plasma membrane
What are the 3 types of coats on vesicles
Clathrin
COP-I and COP-II
Where does calthrin coat transport from to
plasma membran to golgi/endosome
where does the COP-I go from
From cis-golgi to ER
