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Laboratory > Ch 6 RPR & Mono > Flashcards

Ch 6 RPR & Mono Flashcards

1
Q

Rapid Plasma Reagin is a ________ test for serologic detection of the antibody Reagin

A

non-treponemal

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2
Q

Reagin is a phospholipid produced in patients infected with

A

Treponema pallidum

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3
Q

Causative agent of syphilis and acts like an antigen

A

Treponema pallidum

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4
Q

Reagin is found in the _____ of patients with syphilis

A

plasma/serum

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5
Q

Vehicle or antigen

A

Carbon charcoal particle

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6
Q

Reactive result

A

Flocculation

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7
Q

FTA-ABS

A

Fluorescent treponemal antibody-absorbed test

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8
Q

Confirmatory test for RPR

A

FTA-ABS

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9
Q

FTA-ABS

Fixed to a slide and patient’s serum is added

A

Dead T. pallidum

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10
Q

FTA-ABS

Ultraviolet microscope is used, Positive result

A

Fluoresce

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11
Q

RPR

Tube top color used for serum

A

Red/SST

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12
Q

RPR

Tube top color for plasma

A

Purple

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13
Q

After centrifugation, serum should be:

A

Clear and non-hemolyzed

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14
Q

RPR Card Test kit

A

RPR Card antigen suspension

Plastic disposable bottle

20 Gauge, galvanized needles, blunt

Test cards

Pipettes/stirrers

Test Controls

1 mL TB Syringe

Distilled water

Mechanical rotator

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15
Q

RPR

Mix the antigen by:

A

Shaking vigorously for 10-15 seconds

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16
Q

RPR

Dispense number of drops

A

1 drop

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17
Q

RPR

Number of drops for the antigen to each specimen

A

1

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18
Q

RPR test card should be on rotator for:

A

8 minutes at 100 rpm

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19
Q

RPR

Read the reaction time after:

A

8 minutes on rotator

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20
Q

Helps differentiate non-reactive from weakly reactive results

A

Tilting the card by hand (3 to 4 to-and-fro motion)

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21
Q

Read macroscopically under:

A

high intensity lamp or strong daylight

22
Q

RPR

Report results as:

A

Reactive or Non-reactive

23
Q

Reactive RPR tests shows flocculation ranging from:

A

Slight but definite

Minimum to moderate

Marked and intense

24
Q

RPR tests must be confirmed with:

A

FTA-ABS

25
Q

How often are controls ran with each batch of Rapid Plasma Reagin tests?

A

Always

26
Q

Ensures the accuracy and reliability of test results

A

Quality Control

27
Q

Quality Control starts with:

A

Proper collection and handling of specimen

Assaying control reagents

Checking instrument performance

28
Q

Two sections of Quality Control

A

Equipment quality control

Reagent quality control

29
Q

Calibration of the mechanical rotator

A

100 rpm

30
Q

Calibration of the antigen needle

A

60 drops per 1 mL

31
Q

Controls used in RPR

A

Reactive

Weak-reactive

Non-reactive

32
Q

Control reagents are treated as patients and ran every time an:

A

Unknown sample is tested

33
Q

Mononucleosis is caused by

A

Epstein-Barr virus

34
Q

Mono is typically seen in:

A

Young adults and children <5 years old

35
Q

Symptoms of mono

A

Fever

Malaise

Lethargy

Sore throat w/ exudates

Enlarged lymph nodes of neck

Mild hepatitis

Enlarged spleen

Blotchy skin rash

36
Q

Causes the formation of heterophile antibody

A

Increased and abnormal forms of lymphocytes and monocytes

37
Q

An antibody that is structurally similar to the antibody that is specifically produced as a response to antigen stimulation

A

Heterophile antibody

38
Q

Mono blood samples used

A

Serum or plasma

39
Q

Monospot Latex Test Kit

A

Latex Reagent suspension

Pipettes/stirrers

Test Slides

Positive and Negative Control

40
Q

Used to sediment cellular elements

A

Centrifuge

41
Q

Mono

Test the specimen within 24 hours of blood collection if stored:

A

2-8 degrees Celsius

42
Q

Allow the Latex Reagent and controls to reach:

A

Room Temperature (20 to 30 degrees C)

43
Q

Shake the Latex Reagent:

A

Gently

44
Q

Add ___ of Latex Reagent next to the drop of sample

A

1 drop

45
Q

Mono

Gently rotate the slide ___ minutes manually

A

3

46
Q

Mono

Use a rotatory shaker set at:

A

60-100 rpm

47
Q

Mono:

Look for the presence of:

A

Agglutination

48
Q

Mono

Report results as either:

A

Positive or Negative

49
Q

Mono

A negative result with demonstrate:

A

Smooth, homogenous solution with no agglutination

50
Q

Number of controls for monospot

A

2

Positive and Negative

Laboratory (9 decks)

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