Ch. 15- Translation Flashcards
mature mRNA is ‘read’ by _____ to synthesize proteins
ribosomes
____ are used to ‘read’ the mRNA and transport the appropriate amino acid to the ribosome
tRNA
a _____ terminates translation
release factor
What does it mean that the genetic code is degenerate?
more than 1 triplet encodes for the same amino acid
_____ are found on tRNA and are complementary to the codons
anticodons
Watson-Crick pairing rules are relaxed at the ____ base of a codon allows degeneration of the code (what is this called)
3rd base; wobble
The open reading frames goes to
start codon to stop codon
polypeptide synthesis is carried out from the blank terminus to blank terminus
N to C terminus
Aminoacyl Site
where the charged tRNA attaches
Peptidyl Site
peptide bond formed
Ribosome begins translating at
AUG
Kozak sequence
sequence containing AUG
When AUG is found by the small ribosomal subunit…
tRNA^fMet added to the P site and the large ribosomal subunit binds
Translation termination
no tRNA associated with a stop codon; release factor 1 attaches to A site at the stop codon, release factor 3 in complex with GTP binds to ribosome, GTP is hydrolyzed to GDP and the mRNA, tRNA, and RF’s are released from ribosome
How do identify the coding strand
is the strand with the open reading frame