Ch. 11 Protein Engineering Flashcards
What is protein engineering?
Alteration of a protein through genetic engineering to improve the proteins properties
Which alteration to the protein sequence provides a major increase in protein stability?
Introduction of cysteine residues for disulfide bond formation
Which of the following concepts increases protein stability? D
Decreasing the number of possible unfolded conformations
What is a benefit to changing the active site or cofactor binding site of an enzyme?
To prevent a reaction from taking place
Why might it be sensible to simplify an industrially relevant enzyme?
Idk
What term describes the process of creating random mutations in a gene and then selecting for improved function or altered specificity of the resulting protein product?
Directed evolution
Which of the following is required to insert a nonnatural amino acid during in vivo protein synthesis, but is not required for in vitro protein synthesis?
A tRNA charged with the nonnatural amino acid
Which of the following is a way to create novel enzymes?
All of the above
- glycoengineering
- using nonnatural amino acids to add new functional groups to amino acids within binding sites
- recombining functional domains from different enzymes
- DNA shuffling
How were improved B-lactamases made?
By DNA shuffling several related B-lactamase genes from difference enteric bacteria
With regards to exons, how is a combinatorial library constructed?
Idk
Which regards to exons, how is an alternative splicing library created?
Idk
What are ELPs?
Engineered proteins that are similar to elastin
Which of the following is a structural domain associated with protein-protein binding?
All of the above
- B-barrel
- three-helix bundle
- beta sandwich
- peptide binders
Which of the following statements about protein engineering is NOT correct?
The methods that exist to engineer protein sequences are often not useful
Which of the following is not a genetically encoded amino acid?
Tyrosine
