Ch 1 Flashcards
Microbiology revolves around two themes:
- understanding basic life processes
- applying that knowledge to the benefits of humans
___ are excellent models for understanding cellular processes in unicellular and multicellular organisms.
Microbes
Microbes play important roles in ___, ___, and ___.
Medicine, agriculture, and industry
The importance of microorganisms:
- oldest form of life
- largest mass of living material on earth
- carry out major processes for biogeochemical cycles
- can live in places unsuitable for other organisms
- other life forms require microbes to survive
The cell is..
A dynamic entity that forms the fundamental unit of life
Cytoplasmic (cell) membrane:
Barrier that separates the inside of the cell from the outside environment
Cytoplasm:
Aqueous mixture of macromolecules, ions, and ribosome
Ribosomes:
Protein-synthesizing structures
Cell wall:
Present in most microbes; confers structural strength
All cells have the following in common:
- cytoplasmic membrane
- cytoplasm
- ribosomes
- cell wall
Prokaryotes
- no membrane-enclosed organelles, no nucleus
- generally smaller than eukaryotic cells
Eukaryotes
- DNA enclosed in a membrane-bound nucleus
- cells are generally larger and more complex
- contain organelles
Genome:
A cells full complement of genes
Eukaryotic DNA is ___ and found within the ___.
Linear, nucleus
Eukaryotic cells usually have more than one ___.
Chromosome
Eukaryotic cells typically have ___ copies of each chromosome.
Two
During cell division, nucleus divides by ____.
Mitosis
During sexual reproduction, the genome is halved by ___.
Meiosis
Prokaryotic cells generally have a single, circular DNA molecule called a ___.
Chromosome
DNA aggregates to form the ___.
Nucleoid region
Prokaryotes also may have small amounts of extrachromosomal DNA called ___ that confer special properties.
Plasmids
E. coli genome vs. human cell
E. coli
4.64 million base pairs
4,300 genes
Human cell
1,000x more DNA per cell than E. coli
7x more genes than E. coli
Metabolism:
Chemical transformation of nutrients
Reproduction:
Generation of two cells from one
Differentiation:
Synthesis of new substances or structures that modify the cell
Communication:
Generation of, and response to, chemical signals
Movement:
Via self-propulsion, many forms in microbes
Evolution:
Genetic changes in cells that are transferred to offspring
The process of change over time that results in new varieties and species of organisms
Enzymes:
Protein catalysts of the cell that accelerate chemical reactions
Cells store and process information that is eventually passed on to offspring during reproduction through ___ and ___.
DNA and evolution
DNA stands for:
Deoxyribonucleic acid
Transcription:
DNA produces RNA
DNA produces RNA
Transcription
Translation:
RNA makes protein
RNA makes protein
Translation
Growth:
The link between cells as machines and cells as coding devices
Last universal common ancestor (LUCA):
Common ancestral cell from which all cells descended
Earth is ___ years old
4.6 billion
First cells appeared between ___ and ___ years ago
3.8-3.9 billion
The atmosphere was anoxic until about ___ years ago.
2 billion
Metabolisms were exclusively ___ until evolution of oxygen-producing phototrophs
Anaerobic
Life was exclusively microbial until about ___ years ago.
One billion
Phylogeny:
- Evolutionary relationships between organisms
Ribosomal RNA (rRNA) is excellent for determining ___.
Phylogeny
Relationships visualized on a ___ ___.
Phylogenetic tree
Relationships can be deduced by comparing ___ ___ in the different specimens
Genetic information
Comparative rRNA sequencing has defined three distinct lineages of cells called ___.
Domains
Three domains:
Bacteria (prokaryotic)
Archaea (prokaryotic)
Eukarya (eukaryotic)
Archaea are more closely related to ___ than ___.
Eukarya than bacteria