Cellular Respiration Flashcards

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1
Q

What is the main purpose of cellular respiration?

A

To break down food molecules to produce ATP

Cellular respiration includes catabolic reactions.

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2
Q

What is the key equation for cellular respiration?

A

C6H12O6 + 6O2 → 6CO2 + 6H2O + Chemical Energy (ATP)

This equation summarizes the overall process of cellular respiration.

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3
Q

Where does energy originate in the context of cellular respiration?

A

From the Sun, captured via photosynthesis

Energy is stored in sugars.

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4
Q

How is energy in glucose harnessed during cellular respiration?

A

By transferring electrons through a series of redox reactions

This process powers ATP synthesis.

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5
Q

What are the three main stages of cellular respiration?

A
  1. Glycolysis
  2. Pyruvate Oxidation and Citric Acid Cycle
  3. Oxidative Phosphorylation

Each stage has distinct processes and occurs in different cellular locations.

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6
Q

Where does glycolysis occur?

A

In the cytosol

Glycolysis converts glucose into two pyruvate molecules.

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7
Q

What are the products of glycolysis?

A

2 ATP (net) and 2 NADH

Glycolysis is an anaerobic process.

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8
Q

What happens during pyruvate oxidation?

A

Pyruvate is converted into Acetyl-CoA

This occurs in the mitochondrial matrix.

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9
Q

What does Acetyl-CoA enter after pyruvate oxidation?

A

The citric acid cycle (Kreb’s Cycle)

This cycle produces NADH, FADH2, ATP, and CO2.

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10
Q

What is oxidative phosphorylation?

A

Involves the electron transport chain (ETC) and chemiosmosis

It is the final stage of cellular respiration.

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11
Q

What role do NADH and FADH2 play in oxidative phosphorylation?

A

They provide high-energy electrons to the electron transport chain

This process creates an electrochemical gradient.

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12
Q

What is chemiosmosis?

A

The movement of H+ down their concentration gradient through ATP synthase

This movement powers ATP production.

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13
Q

Who is the terminal electron acceptor in cellular respiration?

A

O2

It becomes reduced to H2O.

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14
Q

Fill in the blank: Glycolysis converts glucose into _______.

A

two pyruvate molecules

Each pyruvate molecule has 3 carbons.

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15
Q

True or False: Glycolysis can occur in the absence of oxygen.

A

True

This is why it is classified as an anaerobic process.

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16
Q

What are the two mechanisms of ATP production?

A
  1. Substrate-level phosphorylation
  2. Oxidative phosphorylation
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17
Q

Define substrate-level phosphorylation.

A

Direct transfer of phosphate to ADP

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18
Q

What is oxidative phosphorylation?

A

ATP synthase uses energy from the proton gradient

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19
Q

Which mechanism produces the most ATP?

A

Oxidative phosphorylation

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20
Q

What is the Electron Transport Chain (ETC)?

A

A series of protein complexes and mobile electron carriers involved in oxidative phosphorylation during cellular respiration

Occurs in the inner mitochondrial membrane in eukaryotes and the plasma membrane in prokaryotes.

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21
Q

What are the primary electron sources for the ETC?

A

Electrons harvested from glucose during glycolysis, pyruvate oxidation, and the citric acid cycle, carried by NADH and FADH2

NADH and FADH2 are oxidized back to NAD* and FAD.

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22
Q

What happens to NAD* and FAD after donating their electrons?

A

They are recycled back to glycolysis and the citric acid cycle to get reduced and repeat the cycle.

23
Q

What are the key components of the ETC?

A

Complexes I, II, III, IV

These complexes facilitate the movement of electrons via redox reactions.

24
Q

How do electrons move through the ETC?

A

Via a series of redox reactions through protein complexes, with each complex having a higher affinity for electrons than the previous one.

25
Q

What happens to the energy of electrons as they move down the ETC?

A

They lose free energy, which is used to pump protons (H+) across the inner mitochondrial membrane.

26
Q

What is created as a result of protons being pumped across the inner mitochondrial membrane?

A

An electrochemical gradient, known as the proton motive force.

27
Q

What is the role of oxygen in the ETC?

A

It accepts electrons and combines with H* ions to form water (H2O), acting as the terminal electron acceptor.

28
Q

What would happen to the ETC without oxygen?

A

The chain cannot operate, halting ATP production.

29
Q

Fill in the blank: The energy released as electrons flow is used by Complexes I, III, and IV to pump _______ ions.

A

H+

30
Q

What two types of potentials contribute to the proton motive force?

A

Chemical Potential and Electrical Potential

Chemical potential refers to the high concentration of H+ in the intermembrane space, while electrical potential refers to the positive charge in the intermembrane space relative to the matrix.

31
Q

True or False: The electrons start at a very low energy level when they are associated with NADH or FADH2.

A

False

32
Q

What happens to electrons by the time they reach the terminal electron acceptor?

A

They are at a very low energy level.

33
Q

What drives the rotation of ATP synthase?

A

Protons flow back into the matrix through ATP synthase

This flow of H+ acts like a turbine, enabling ATP synthesis.

34
Q

What is the primary function of ATP synthase?

A

Catalyzes the phosphorylation of ADP to ATP

ATP synthase converts ADP into ATP using the energy from the proton gradient.

35
Q

Which electron carrier contributes more energy to proton pumping?

A

NADH

NADH enters the electron transport chain at Complex I, while FADH2 enters at Complex II.

36
Q

At which complex does FADH2 enter the electron transport chain?

A

Complex II

FADH2 contributes less energy compared to NADH due to its entry point.

37
Q

What ensures efficient energy capture in the electron transport chain?

A

The gradual energy drop of electrons

This process minimizes excessive heat loss during energy transfer.

38
Q

What are the key molecules involved in respiration?

A
  • NADH
  • FADH2
  • ATP Synthase

NADH and FADH2 shuttle high-energy electrons, while ATP synthase generates ATP.

39
Q

What process allows ATP synthase to generate ATP?

A

Passive flow of H+ ions down their concentration gradient

This process utilizes the free energy released during the flow.

40
Q

What is feedback inhibition in the context of respiration?

A

Products of the pathway inhibit upstream enzymes, ensuring regulation based on cellular energy needs.

41
Q

What is lactate fermentation?

A

Produces lactic acid (in animals and some bacteria).

42
Q

What is alcoholic fermentation?

A

Produces ethanol and CO2 (in yeast).

43
Q

What is the primary reason fermentation exists?

A

To recycle NAD+ molecules.

44
Q

Why does NADH accumulate in the absence of oxygen?

A

Because NADH can’t donate its electrons to the ETC.

45
Q

What happens to NAD+ in the absence of oxygen?

A

NAD+ molecules become depleted, preventing glycolysis and the citric acid cycle from proceeding.

46
Q

What is the end result of depleted NAD+ during respiration?

A

No ATP production.

47
Q

Where does respiration occur in prokaryotes?

A

In the cytoplasm and plasma membrane.

48
Q

What are the types of organisms based on their oxygen requirements?

A

Strict aerobes, strict anaerobes, and facultative anaerobes.

49
Q

Fill in the blank: Fermentation is an alternative pathway when _______ is unavailable.

A

oxygen

50
Q

What are Reactive Oxygen Species (ROS)?

A

Byproducts of aerobic respiration that can damage cells

ROS are harmful and require management by the cell’s antioxidant systems.

51
Q

How are Reactive Oxygen Species (ROS) managed in cells?

A

Managed by antioxidant systems like enzymes (e.g., catalase)

Antioxidant systems help neutralize ROS to prevent cellular damage.

52
Q

True or False: Anaerobic prokaryotes cannot use oxidative phosphorylation.

A

False

Some anaerobic prokaryotes use molecules other than O2 as the terminal electron acceptor.

53
Q

Fill in the blank: Some anaerobic prokaryotes are able to use oxidative phosphorylation because they use _______ as the terminal electron acceptor.

A

[molecules other than O2]