cells Flashcards
prokaryotic cell
lack many membrane bounded organelles such as Mitochondria, Endoplasmic reticulum, Chloroplast and Golgi Apparatus (trick: mec-g)
Ribosomes
prokaryotes: 70s (small)
eukaryotic: 80s (large)
prokaryotic cell
- mitosis & meiosis is missing so binary fission takes place in cells
-have no true nucleus so dna scatter in the cytoplasm - chromosomes is formed of only dna
- cell wall is made of peptidoglycan and murein
-Average diameter 0.5 – 20 μm of cell
eukaryotic cells
- its flagella and cillia are made up of microtubules
- a typical cell’s size is 10 - 100um
- chromosomes is made up of dna and protein
-plant cell wall made of cellulose and fungi cell wall is made of chitin
-Complex, with 9 + 2 arrangement
of microtubules intracellular,200 nm diameter
Microscope
- The discovery of cells and their structures become possible with the development of optical lenses and with construction of compound microscope ( mikros- small, skopein- to see, to look)
- Which was invented by David Jensen in 1590 and in 1610 Galileo an Italian astronomer and physicist designed it properly
Cell (greek, kytos- cell; la; cella- hollow space
- It was discovered by Robert hooke in 1665
- Schwann after finding nucleus in the centre of the cell nucleus surrounded by a fluid in the cell his observation changed the definition of cell it described that
“cell as a structure which consist of nucleus surrounded by semi fluid substance in closed by a membrane”
Amount of cell
- When Robert hook first discovered the cell as a basic unit of life in 1663 he calculated over 1 billion of cells in a cubic inch of cork.
Some of the people have done research on the no of cells in a way that - A newborn human baby contain two trillion cells.
- An adult 16 trillion when you donate blood.
- When you donate blood, you give away 5.4 billion cells.
- Each day infact a human body sloughs off and replaces 1 percent of its cells or about 600 billion
Nucleus
- ## Discovered by Robert Brown (1831)
Emergence of cell theory
In 1838 schleiden a German botanist concluded that despite differences the structure of various tissues and plants were made of cells, in 1839 a German biologist T. Schwann published the comprehensive report on cellular basis of animals, in 1855 Rudolf Virchow a German pathologist had made a convincing case and added third point to the cell theory:
1. All organisms are composed of one or more cells.
2. The cell is the structural and functional unit of life.
3. Cells can arise only by division of pre existing cells it is not a ‘denovo’ structure.
Vacuoles
- Generally vacuoles (except food vacuoles) are non protoplasmic liquid filled cavities in the cytoplasm
- Surrounded by a membrane called tonoplast (selectively permeable)
- The vacuoles in plant cells are filled with cell SAP and act as store house which often place role in plant defence. Plant vacuoles sometimes act as lysosome as they contain hydrolytic enzyme after death cells tonoplast lose its differential permeability and its enzyme causes lysis of these cell.
- In animal cells lysosomes are which in hydrolytic enzyme including proteases, ribonucleases and glycosidase.
Cell membrane
Plasma membrane or cell membrane is the outer most boundary of the cell. However, in most plant cells, it is covered by a cell wall.
Structure
Under electron microscope it is a bi-
layered structure, this structure is
delicate and elastic it has some ability
to repair itself.
Models of Cell Membrane Structure
There are two models of cell
membrane structure:
Unit Membrane Model According to
this model the cell membrane
is composed of lipid bilayer
and sandwiched between two
protein layers. This structure is
present in all cell organelles
(for example, mitochondria,
chloroplast etc.)
Fluid Mosaic Model According to the fluid mosaic model the cell membrane consists of a double
layer of phospholipid molecules, known as a lipid bi-layers. It has proteins and other molecules.
The name fluid mosaic is used because the bi-layer is a very fluid structure and it contains a mosaic
of protein molecules.
Chemical Composition
Cell membrane contains phospholipids 20-40% proteins, 60-80% cholesterol and
polysaccharides.
• It is about 7 nm thick
• The basic structure is a phospholipid bilayer.
• The hydrophilic phosphate heads of the phospholipids face outwards. The result is the
aqueous environment inside and outside the cell.
• The hydrocarbon tails face inwards and create a hydrophobic interior.
• Most protein molecules float about in the phospholipid bilayer forming a fluid mosaic pattern.
• The proteins stay in the membrane because they have regions of hydrophobic amino acids
which interact with the fatty acid tails to exclude water.
• Some proteins and lipids have short branching carbohydrate chains like antennae forming
glycoproteins and glycolipids respectively.
• Membrane also contains cholesterol. Like unsaturated fatty acids cholesterol disturbs the
close packing of phospholipids and keeps them more fluid. This can be important for
organisms living at low temperatures when membranes can solidify. Cholesterol also
increases flexibility and stability of membranes, without it membrane break up.
• The two sides of a membrane differ in composition and function.
Functions of Membrane
• The phospholipid bilayer provides the basic structure of membrane. It also restricts entry and
exit of polar molecules and ions.
• Channel protein and carrier proteins are involved in the selective transport of polar
molecules and ions across the membrane.• Some proteins act as receptor molecules for chemical signaling between
cells.
• Some proteins act as an antigen these act as cell identity markers.
• Glycolipids and glycoproteins help cells to recognize each other – allowing
the immune system to tell the difference between body cells and invading
bacteria.
• Energy transfer ion photosynthesis and respiration proteins take part in the
energy transfer system.
Imp points 1
•diffusion
and passive transport are the two names of the same process
•ER with no ribosomes attached
is known as SER.
Smooth ER is not involved in
protein synthesis but is the site
of steroid (lipid hormone)
production. It also contains
enzymes that detoxify, or
make harmless, a wide variety
of organic molecules, and it
acts as a storage site for
calcium in skeletal muscle
cells.
•Discovery of cell is linked with the
invention of microscope because most of the cells are microscopic
Jean Baptist de-Lamarck (1809) said
“nobody can have life if it is not
formed by cellular tissue.”
•Robert Brown said that nucleus is
present in the cell and the cell is not
an empty space. He discovered the
nucleus in the cells of orchids.
•Lorenz Oken in 1805 (a German
scientist) said “all living beings
originate from or consist of vesicles or cells”.
•A cell is the structural and
functional unit of life
•Louis Pasteur (1862)
said that bacteria
are formed from
existing bacteria.
•Jean Baptist de-Lamarck (1809) said
“nobody can have life if it is not
formed by cellular tissue.”
Diffusion
Diffusion is the movement of molecules
or ions from a region of their high
concentration to a region of their low
concentration. The process is passive
(does not require energy and happens
spontaneously).
Two factors affect the rate of diffusion
a) Difference in concentration between
point A and point B. The steeper the
gradient, the faster the rate of diffusion.
b) The greater the surface area of a
membrane through which diffusion is
taking place the greater the rate of
diffusion.
Facilitated Diffusion
Some substances enter and leave cells
much faster than you would expect it
only diffusion occurred. We now know
that some membrane proteins facilitate
the diffusion of some substances across
the cell membrane.
Two types of proteins are responsible
for facilitated diffusion.
• Specific carrier protein takes
particular substance from the
membrane to the other.
• Ion channels are proteins that
open and close to control the
passage of selected charged
articles.
• Channel proteins have a fixed
shape
Osmosis
Osmosis is a passage of water molecules from a region of their high concentration to a
region of their low concentration through a partially permeable membrane. We can say
that it is a form of diffusion in which only water molecules move.