Cell wall synthesis inhibitors (Overview)

Question 1

What peptidoglycan strands make up the cell wall in bacteria?

Answer 1

N-acetylmuramic acid (NAM)

N-acetylglucosamine (NAG)

Question 2

What is the site for Beta lactam action?

Answer 2

Transpeptidases

Transpeptidases cross-link adjacent strands to give lattice structure for wall

Question 3

What are PBP’s?

Answer 3

Penicillin binding proteins
Transpeptidases, carboxypeptidase, transglycosylase
They are responsible for maintenance and regulation of peptidoglycan cell walls

Question 4

What does PBP 1 do?

Answer 4

Elongation of cell wall

Question 5

What does PBP 2 do?

Answer 5

Maintains rod shape

Question 6

What does PBP 3 do?

Answer 6

Formation of septum on division

Question 7

How many PBP’s does e. coli have?

Answer 7

Up to 7

Question 8

How many PBP’s does staph aureus have?

Answer 8

4

Question 9

How do beta lactams work?

Answer 9

They form covalent bonds with PBP’s, transpeptidases, inhibiting activity

Question 10

How does vancomycin work?

Answer 10

Binds end of polypeptide chain to prevent elongation

Question 11

How does bacitracin work?

Answer 11

Inhibits lipid carrier of peptidoglycans that carry them outside of the cell