cc (centri) Flashcards
It is a process in which centrifugal force is used to separate solid matter from a liquid suspension.
CENTRIFUGATION
– moves particles away from the axis of rotation
Centrifugal force
- is used to prepare samples, blood, and body fluids, in clinical chemistry for analysis
Preparative centrifugation
- is used to concentrate urine sediment in urinalysis for microscopic viewing.
Analytical centrifugation
When samples are not properly centrifuged, ____ can cause erroneous results during analysis.
small fibrin clots and cells
are generally used to separate serum or plasma from the blood cells as the blood samples are being processed
Centrifuges
Is used to separate a supernatant from a precipitate during an analytic reaction
Centrifuges
–is attached to the vertical shift of a motor or air compressor and enclosed in a metal covering
Head/rotor assembly
– holds the test tubes
Centrifuge tube holder
– encloses the tube holder and head of the centrifuge
Chamber
– secures the cover of the entire chamber
Latch
- supplies the force to the shaft and rotor in order to achieve the desired speed and create sufficient centrifugal force
Motor
– used to decelerate the centrifuge machine
Braking device
2 Types of Centrifugal Forces:
Buoyant force-
Frictional force
force required to displace the particles from the liquid media into which they settle
Buoyant force-
force generated by particles as they migrate through the solution
Frictional force –
– density of both samples and solution
Mass
is expressed in revolutions per minute (rpm)
Speed -
– from center of rotor to sample
Radius
Speed of the centrifuge was verified using?
tachometer or strobe light
AKA swinging-bucket type
Horizontal Centrifuge
When at rest or not in motion, the centrifuge tubes are held vertically, but shift to a horizontal position when fully in motion or when the centrifuge is turned on
Horizontal Centrifuge
AKA angle-head centrifuge
Tube angle is 25-40 degrees
Vertical Centrifuge
the type of centrifuge with the highest speed
It follows a fixed angle but it produces a very tightly packed sediment
Ultracentrifuge –
Recommended temperature is -15°C to -25°C
Method of choice for separating fractions of lipoproteins and drug-binding assays
Ultracentrifuge –
AKA Cytospin centrifuge
It is used to prepare body fluids, such as blood, urine, peritoneal, pleural, pericardial, and cerebrospinal fluid (CSF), as well as fine needle aspirates that can be spread on the slide
Cytocentrifuge –
Maintenance of Centrifuge
Weekly/Biweekly-
monthly
quarterly
