CBC

Question 1

What is MCV conceptually?

Answer 1

Average volume of a RBC.

HCT= RBC x MCV if determined via voltage pulses

When manually determined 10* HCT= RBC x MCV

Question 2

What is MCH?

Answer 2

Mean Corpuscular Hemoglobin
-The amount of hemoglobin per RBC

Calculated by Hgb/RBC

Question 3

MCHC

Answer 3

Mean Corpuscular Hemoglobin Concentration
-[Hemoglobin] per volume of cells.

Hgb/Hct*100

Question 4

How does flow cytometry work?

Answer 4

It uses lasers and fluorescent light to measure size and content of cells through scattering effects.

Gives you the scatter plot graph.

Question 5

Why might reticulocytes be important to measure?

Answer 5

Measures RBC production. May be helpful to measure in people with anemia.

Question 6

What are the 3 components of blood after centrifuge?

Answer 6

1. RBC- Hematocrit
2. Buffy Coat- WBC and Platelets
3. Plasma

Question 7

How can hematocrit be estimated?

Answer 7

Hct= RBC x MCV

Question 8

How does Aperture impedance work?

Answer 8

Electrical current is established. When a
cell or particle passes through the aperture, the cell or particle changes the current flow and
causes a voltage surge. The voltage, first rising above and then falling below a certain threshold,
indicates that a cell or particle has passed through the sensing zone.

Question 9

What are the hallmarks of a Neutrophil on a slide?

Answer 9

Poly Nuclear

Question 10

How do you identify a lymphocyte on a smear?

Answer 10

Look for mostly Nucleus

Question 11

How do monocytes appear on a slide?

Answer 11

Largest cell
Irregular shaped nucleus (not PMN)
Vacuoles

Question 12

What are the hallmarks of an eosinophil on a slide?

Answer 12

Red granules in cytoplasm

Question 13

What are the hallmarks of a basophil on a slide?

Answer 13

Almost completely covered in blue granules.