Antibody

Question 1

What is the difference between an Fc and a Fab region?

Answer 1

Fab= Fragment for antigen binding

Fc doesn’t bind. its conserved.

Question 2

F(ab)2 has a valance of…

Answer 2

2. It can bind 2 antigens.

Fc has a valance of 0.

Question 3

The part of the antigen that interacts with the antibody is known as the.

Answer 3

Epitope.

Question 4

What is the purpose of the sugar in between the 2 heavy chains?

Answer 4

It keeps the chains from collapsing on each other.

Question 5

Give an example of a use for an Idiotype antibody

Answer 5

In auto immune diseases such as Graves. You could create an idiotype to bind to the variable region of the self binding antibody, thus creating a antibody against the antibody,

Question 6

Which type of antibody forms dimers?

How many peptide strands are on it?

Answer 6

IgA

4 Light
4 Heavy
1 J
1 Secretory

10 Total

Question 7

Which type of antibody forms a pentamer? What is the valance?

Answer 7

IgM M=macro

10

Question 8

Humoral immunity is…

Answer 8

Antibody immunity.

Question 9

What is an immune complex?

Answer 9

Antibody+Antigen

Question 10

Which antibody is the first responder to a new antigen?

Answer 10

IgM because it activates compliment protein C1g.

Question 11

What is the only antibody the human fetus can make?

Answer 11

IgM

Question 12

What is the concentration and half life of IgG? What is unique about IgG

Answer 12

1000mg/dL

3 weeks

It crosses the placenta.

Question 13

Where would you most likely find IgA?

Answer 13

In mucosal layers of the body

Question 14

Where would you most likely find IgD?

Answer 14

As receptors on B cells

Question 15

What does IgE do?

Answer 15

Helps mediate Parasites.

Question 16

What is the sequence of activation in the compliment cascade?

Answer 16

1,4,2,3,5,6,7,8,9

Note the 4*

Question 17

T/F the alternative pathway of the compliment cascade is innate immunity.

Answer 17

True.

It is not activated by antibodies but rather bacteria.

Question 18

Which complient proteins become the Membrane attack complex?

Answer 18

6-9.

The MAC will punch holes in the foreign body

Question 19

What is opsonization?

Answer 19

Where a Polynuclear Macrophage eats a bacteria because of the IgG “handles” that bind to the antigens.

Question 20

What is responsible for the chemotactic response for polymorphonuclear neutrophils

Answer 20

C5a

Question 21

What is responsible for the anaphylatoxic response in the compliment cascade?

Answer 21

C3a C4a C5a.

Release histamine from mast cells and basophils

Question 22

On an electrophoresis separation of blood plasma, what is seen from + to -? Where are antibodies located?

Answer 22

\+
Albumin
A1
A2
B1
B2
Gamma** antibodies here
-

Question 23

Which chain of an antibody is responsible for its classification?

Answer 23

The H-chain (gamma, alpha, mu, epsilon, delta)

Question 24

Kappa and lambda chains are associated with ___________ and (do or do not) change during cell switching.

Answer 24

Kappa and lambda chains are associated with LIGHT CHAIN and DO NOT change during cell switching.

Question 25

What are the concentrations of IgG IgA IgM in serum?

Answer 25

1000-G
200-A
100-M

Question 26

Which antibody can cross the placenta,
activate complement, involved in allergic diseases, “first line of defense”, and most resistant to
enzymatic digestion.

Answer 26

Placenta- IgG
Compliment- IgM, IgG
Allergy- IgE
Digestion protection- IgA
First line of defense- IgM

Question 27

What mediates the lectin pathway of the compliment?

Answer 27

Mannose Binding Protein MBP or MBL.

MBP takes the spot of C1 in the classical cascade.

Question 28

What are the 4 actions of compliment?

Answer 28

1. Lytic - MAC (membrane attack complex)
2. Opsinization -FcR (Fc Receptor for phagocytosis)
3. Chemotactic- C5a
4. Anaphyltoxic- C3-5a

Question 29

What is/are the key component of the Lytic compliment?

Answer 29

Lytic - MAC (membrane attack complex)

Question 30

What is/are the key component of the opsinization compliment?

Answer 30

Opsinization -C3 activation FcR (Fc Receptor for phagocytosis)

Question 31

What is/are the key component of the Chemotactic compliment?

Answer 31

Chemotactic - C5a

Question 32

What is/are the key component of the Anaphyltoxic compliment?

Answer 32

Anaphyltoxic- C3-5a

Question 33

What is a toxoid?

Answer 33

An altered form of a toxin that the body can use to develop Antibodies towards. The antibodies work on the active toxin.

Question 34

What changes during “class switching” of antibodies?

Answer 34

the Heavy Chain C domain.

Question 35

Which compliment protein polymerizes and punches holes in the bacteria?

Answer 35

C9