Antibody Flashcards
What is the difference between an Fc and a Fab region?
Fab= Fragment for antigen binding
Fc doesn’t bind. its conserved.
F(ab)2 has a valance of…
- It can bind 2 antigens.
Fc has a valance of 0.
The part of the antigen that interacts with the antibody is known as the.
Epitope.
What is the purpose of the sugar in between the 2 heavy chains?
It keeps the chains from collapsing on each other.
Give an example of a use for an Idiotype antibody
In auto immune diseases such as Graves. You could create an idiotype to bind to the variable region of the self binding antibody, thus creating a antibody against the antibody,
Which type of antibody forms dimers?
How many peptide strands are on it?
IgA
4 Light
4 Heavy
1 J
1 Secretory
10 Total
Which type of antibody forms a pentamer? What is the valance?
IgM M=macro
10
Humoral immunity is…
Antibody immunity.
What is an immune complex?
Antibody+Antigen
Which antibody is the first responder to a new antigen?
IgM because it activates compliment protein C1g.
What is the only antibody the human fetus can make?
IgM
What is the concentration and half life of IgG? What is unique about IgG
1000mg/dL
3 weeks
It crosses the placenta.
Where would you most likely find IgA?
In mucosal layers of the body
Where would you most likely find IgD?
As receptors on B cells
What does IgE do?
Helps mediate Parasites.
What is the sequence of activation in the compliment cascade?
1,4,2,3,5,6,7,8,9
Note the 4*
T/F the alternative pathway of the compliment cascade is innate immunity.
True.
It is not activated by antibodies but rather bacteria.
Which complient proteins become the Membrane attack complex?
6-9.
The MAC will punch holes in the foreign body
What is opsonization?
Where a Polynuclear Macrophage eats a bacteria because of the IgG “handles” that bind to the antigens.
What is responsible for the chemotactic response for polymorphonuclear neutrophils
C5a
What is responsible for the anaphylatoxic response in the compliment cascade?
C3a C4a C5a.
Release histamine from mast cells and basophils
On an electrophoresis separation of blood plasma, what is seen from + to -? Where are antibodies located?
\+ Albumin A1 A2 B1 B2 Gamma** antibodies here -
Which chain of an antibody is responsible for its classification?
The H-chain (gamma, alpha, mu, epsilon, delta)
Kappa and lambda chains are associated with ___________ and (do or do not) change during cell switching.
Kappa and lambda chains are associated with LIGHT CHAIN and DO NOT change during cell switching.
