Carbs Lab Flashcards
simplest ketose, a ketotriose
dihydroxyacetone
simplest aldose where D represents the right handed configuration of sugar
d-glyceraldehyde
dihydroxyacetone is chiral/achiral
achiral
useful qualitative test for the presence of starch
iodine test
strong base that can be used to treat cellulose and then exposing it to carbon disulfide fumes to yield
films and threads
NaOH
Four qualitative tests used in carbohydrates
Barfoed’s
Seliwanoff’s
Benedict’s
Lugol’s
utilizes cupric acetate in dilute acetic acid solution
Barfoed’s test
Barfoed’s test reagent
Cupric acetate in dilute acetic acid solution
React with monosaccharides in general which results to formation of sugar acid and brick-red or rust-colored copper oxide precipitate
Barfoed’s test
Barfoed’s test positive shows
red precipitate
red precipitate in barfoed’s test tells us that
monosaccharide is present
uses resorcinol in dilute hydrochloric acid solution
Seliwanoff’s test
Seliwanoff’s test reagent
resorcinol in dilute hydrochloric acid solution
Reacts with ketoses to form cherry red dye or conjugate upon heating
seliwanoff reagent
seliwanoff’s test show positive when
cherry red dye or conjugate appears
involves the addition cupric sulfate in sodium carbonate and sodium citrate solution to the sample
Benedict’s test
Test for reducing sugars, whether monosaccharides or disaccharides, react with this reagent as manifested in the formation of sugar acid and brick-red or rust-colored copper oxide precipitate
Benedict’s test
Benedict’s test will be positive in the presence of r
reducing sugars
benedict’s test reagent
cupric sulfate in sodium carbonate and sodium citrate solutionbe
benedict’s test will turn positive in what color
red (large amount)/orange (moderate)
uses iodine in potassium iodide to specifically detect the presence of starch or glycogen
Lugol’s test
Lugol’s test reagent
iodine in potassium iodide
Lugol’s test is used to detech the presence of whatr
starch/glycogen
Evident in formation of blue-black dye complex for starch and blue-brown dye for glycogen
Lugol’s test
color of benedict when 0.5% of reducing sugar exist
green
color of benedict when 1% of reducing sugar exist
yellow
color of benedict when 1.5% of reducing sugar exist
orange
color of benedict when 2% of reducing sugar exist
red