Carbohydrates Chapter 3.3 Flashcards
General formula
Cx(H20)y
Monosaccharides
Glucose, Fructose, Ribose
Disaccharides
Sucrose, Lactose, Maltose
Lactose
Glucose+Galactose
Sucrose
Glucose+Fructose
Maltose
Glucose+Glucose
Polysaccharides
Starch, Glycogen, Cellulose
Glucose is a hexose monosaccharide
This means it’s composed of 6 carbons
Structural variations of glucose
There is alpha glucose and beta glucose, both have hydroxyl groups in different places
Properties of glucose
It is polar and soluble. This is down to the hydrogen bonds between the hydroxyl groups. They are polar so are easily attracted to each other because of the partially positive and negative regions.
Condensation reaction
Happens when two hydroxyls from alpha glucose molecules join together and form a glycosidic bond as well as water which is why it’s called that.
Hydrolysis Reaction
Occurs when glucose is need by the organism and is taken from the glucose store (either starch or glycogen), water is added for this to happen which breaks the glycosidic bond.
Amylose
Chain of alpha glucose molecules joined together which twist to form a helix. They also have hydrogen bonds in between the 2 strands and is less soluble than glucose.
Amylopectin
This is like amylose except there are bonds between carbon 1 and carbon 6 that occur every 25 glucose subunits. This results in branching, this makes it more compact. It’s insoluble.
Glycogen
Forms more branches than amylopectin, but same structure. This is because it has to be more compact for animals as they are mobile and need room for other things. It also has free ends on the branches for glucose to be added or take away.
Cellulose
A chain of beta glucose except every alternate one is turned 180 degrees. This can only form straight chains with no branches and it’s insoluble.
Reducing sugars
All monosaccharides and some disaccharides are reducing sugars because they can donate electrons easily.
Testing for reducing sugars
Add Benedicts reagent that contains Cu2+ ions to your sample and heat gently. If there are reducing sugars it will turn brick red because the Cu2+ ions have become reduced to Cu+ ions. The most common non-reducing sugar is sucrose.
Testing for starch
Add a few drops of iodine to potassium iodide solution and add that to your sample. If it goes from yellow/brown to blue/black, it contains starch
Manufactured reagent test strips
These can be used on your sample to test for glucose normally. These will turn a specific colour and you can compare that colour to find a concentration
Colorimeter
These are used to tell you the % transmission or % absorbance of your sample. You can then work out the concentration from that. The more concentration your sample has, the more absorbance and less transmission
Which disaccharide is a non-reducing sugar and how do you make it reducing?
Sucrose is the most common but if you add hydrochloric acid and boil it, it will undergo a hydrolysis reaction which will break the sucrose into glucose and fructose.