carbohydrates Flashcards
general formula for carbohydrates
cx(h20)y
types of carbohydrates
monosaccharide, disaccharide and trisacccharides
example of monosaccharides
glucose, fructose and galactose
example of disaccharides
maltose - glucose + glucose
sucrose - glucose + fructose
lactose - glucose + galactose
polysaccharides
starch, glycogen and starch
bio chemical test for presence of sugar
Benedicts test
reducing sugars
called so because they are involved in a reducing reaction. includes all monosaccharides and some disaccharides as well.
Test for reducing sugars
- add benedicts regaent to the solution and heat it in a water bath.
- if the color changes from clear blue to green, yellow, oranga and red brown due to the presence of insoluble copper oxide ppt, then it is positive.
Test for non reducing sugars
- non reducing sugar has to be broken down into monosaccharides first
- heated with hcl and then is made alkaline using an alkaline solution with sodium hydroxide.
- then is treated with benedicts reagent and heated in a water bath.
- if there is presence of red color then its positive for presence of non reducing sugars.
Test for starch
iodine test
iodine solution is orange brown in color. add a drop of iodine solution to the solid or liquid which is to be tested. if the color changes to blue black, then it indicates the presence of iodine.
why is starch suited for its role
- helix shape making it compact so it can fit a large amount of glucose in a small space.
branched structure increases the surface area for rapid hydrolysis back to glucose. - insoluble in water - doesnt affect the water potential.
- it is a large molecule so it can not cross across the membrane.
why is glycogen suited for its role
glycogen has 1-4 and 1-6 glycosidic bonds making it more branched which makes it more compact and suitable for storage.
- branching enables more free ends where glucose molecules can be added or removed.
monosaccharides
monosaccharides are the monomers from which larger carbohydrates are made.
what type of bond is formed when two monosaccharides are joined together?
glycosidic bond.
polysaccharides definition
polysaccharides are formed by the combination of many glucose units.