Brucella and Legionella Flashcards
Brucella is normal flora of (2)
urinary tract and GI tract of sheep/goat
Brucella characteristics (4)
Non Motile,
Non Encapsulated,
strict aerobe,
intracellular (inside phagocyte)
Brucella
Catalase
Oxidase
Urease
Nitrate
Catalase (+),
Oxidase (+)
Urease (+)
Nitrate (+)
Brucella growth factor
Erythritol (placental tissue)
Brucella Incubation period
2-5 months
Brucella Specimen (4)
blood
bone marrow
tissues,
bronchoalveolar lavage
Brucella category
Class B of bioterrorism
B. abortus (2)
● Bang’s bacillus or Malta fever - cattles
● Less invasive
B. suis (3)
● Swine or pigs
● Highly invasive
● Can cause necrosis
B. canis (2)
● Dogs
● Mild to localized infections
B. melitensis diseases (7)
Undulant fever,
Malta fever,
Gibraltar fever,
Mediterranean fever,
Rock fever,
Gastric Remittent fever,
Crimean fever Maltese fever
Most serious and Debilitating infection
B. melitensis
B. melitensis Optimal specimen
Blood
B. melitensis Definitive Test
Bone marrow culture
Rose Bengal Test
B. melitensis
B. melitensis media (4)
○ TSB
○ Castaneda Broth - best medium
○ BBA
○ W media - specific
Facultative intracellular pathogen
LegionellaLegionella
Legionella
Motility,
Catalase
Weakly Oxidase
Gelatinase
Motile
Catalase (+)
Weakly Oxidase (+);
Gelatinase (+)
Legionella specimens (4)
sputum,
pleural fluid,
blood,
lung biopsy
Most common human pathogen
L. pneumophila
Where can you find L. pneumophila (5)
Air Conditioning ducts,
cooling towers,
plumbing system,
shower heads,
nebulizers
L. pneumophila disease (2)
● Legionnaire’s disease
● Pontiac fever
broad strict pneumonia
Legionnaire’s disease
resembles allergic (non fatal)
Pontiac fever
