ANAEROBES Flashcards
Major components of normal microflora in the body
ANAEROBES
Indicators of Anaerobic bacteria
● ________ (several types) results on Gram stain or culture
● ____ in pus or infected tissues
● ________ of pus or infected tissues
● _____ infected tissues
● Presence of ________ (_______, _______, _______)
● _______ fluorescence (_______, ________)
● Absence of ________
Polymicrobial (several types) results on Gram stain or culture
● Gas in pus or infected tissues
● Foul odor of pus or infected tissues
● Necrotic infected tissues
● Presence of sulfur granules (Actinomyces, Propionibacterium, Eubacterium)
● Brick red fluorescence (Prevotella, Porphyromonas)
● Absence of superoxide dismutase (SOD)
Discouraged for anaerobic culture
Anaerobic swab
Least favorable method of collection
Anaerobic swab
Throat swab for anaerobic culture
● Rejected for we have many _______
● ______ transported after collection
● Use ________ if collection is unavoidable
○ 1st tube – _____________
○ 2nd tube – _____________
● Rejected for we have many anaerobes in throat
● Immediately transported after collection
● Use two tube system if collection is unavoidable
○ 1st tube – contains swab in oxygen free CO2
○ 2nd tube – contains pre reduced anaerobically sterilized culture media
Acceptable specimens (7)
● Bone marrow aspirate
● Blood
● Lung aspirate or biopsy
● Suprapubic aspirate
● Body fluids
● Transtracheal aspirate
● Wound
Unacceptable specimens (5)
● Midstream catch urine
● Catheterized urine
● Sputum
● Feces
● Bronchial washings
Anaerobic Cultivation
● _______
● Indicator: _______ and ______
● Incubation: _____________
○ Check the plates after ______ for ________
● Gaspak Jar /
● Indicator: Reduced Methylene Blue (colorless) and Resazurin (pink)
● Incubation: 35-37 deg Centigrade
○ Check the plates after 24 hours for 5-7 days
Biochemical Tests (7)
- Bile solubility
- Esculin, starch, gelatin hydrolysis
- Catalase
- Lipase
- Lecithinase
- Indole production
- Nitrate reduction CHO fermentation
● Needs 20% bile
● Resembles safety pin
Bacteroides fragilis
● Requires formate and fumarate for growth in broth cultures; pitting of agar
● same disk pattern as Fusobacterium
B. urealyticus
Most common bacteria found in human gut flora; opportunistic pathogen
B. thetaiotaomicron
Can develop 3 colony types
Fusobacterium nucleatum
greening of agar on exposure to air; fusiform rod
Fusobacterium nucleatum
Fusobacterium nucleatum 3 colony types
(smooth, speckled, bread-crumb type);
