Bone Marrow Flashcards
Bone marrow is located within
cavities of the cortical bones
Red and Yellow marrow
a. infant and newborn
b. adult (30-70 yrs old)
c. elderly (>70 yrs old)
a. all red bone marrow
b. 50 % red; 50% yellow
c. >yellow, < red; adipocytes increased 10% per decade
M:E ratio of the following screnario
a. infection
b. leukemia
c. myeloid hyperplasia
d. myeloid hypoplasia
e. erythroid hyperplasia
f. erythroid hypoplasia
a. 6:1
b. 25:1
c. 20:1
d. 3:20
e. 1:20
f. 5:1
ratio of red marrow to yellow marrow
marrow cellularity
Bone marrow smear preparation
a. most commonly used site
b. occasionally used site
c. very rarely used site
d. newborns and infants
a. posterior iliac crest
b. sternum
c. anterior iliac crest and spinal/vertebral bodies
d. upper end of tibial bone
Trephine (core) biopsy
Trephine biopsy needle
Jamshidi needle
Westerman-Jensen needle
Bone Marrow aspirate
Aspiration needle
University of Illinois sternal needle
a. Most predominant in the bone marrow
b. Largest cell in the bone marrow
c. Largest in the peripheral blood
A. Metamyelocyte (juvenille)
B. Megakaryocyte
C. Monocytes
Two most common erythrocytic stages with “fried egg appearance”
Polychromatophilic
Orthochromic normoblast
Bone forming
Water-bug or cornet appearance
Mistaken as plasma cells
Osteoblast
Bone resorption; destroying cells
Mistaken as megakaryocyte
Osteoclast
Identification and origin of HSCs can be determined by “immunophenotypic analysis” using
Flow cytometry
Functional characterization of HSCs can be accomplished “in vitro techniques” using
Culture assay
Cytokines that exert negative influence on hematopoiesis
TGF-Beta
TNF-Alpha
Interferons
Cytokines that exert positive influence on HSC and progenitor cells
KIT Ligand
FLT3 Ligand
GM-CSF
IL-1
IL-3
IL-6
IL-11
