Blood Smear Preparation

Question 1

Two cover slide smears; used for bone marrow sample

Answer 1

Ehrlich

Question 2

Cover slide and glass slide; used for bone marrow sample

Answer 2

Beacom

Question 3

Most preferred method that uses two glass slide; spread with 35-45 degree angle

Answer 3

Wedge smear

Question 4

glass slide size

Answer 4

3x1 inch
(75mm x 25mm x 1-1.2mm thick)

Question 5

Smear for platelet, SLE and WBC count

Answer 5

buffy coat smear (spun smear)

Question 6

Gold standard for blood parasites (malaria)

Answer 6

thick blood smear

Question 7

semi automatic; stimulates the manual wedge technique

Answer 7

Miniprep

Question 8

a. patient with increased hematocrit
b. angle of spreader

Answer 8

a. polycythemia vera
b. lowered to 25 degree angle

Question 9

a. patient with decreased hematocrit
b. angle of spreader

Answer 9

a. anemia
c. higher to 45 degree angle

Question 10

a. Thin smear
c. Thick smear

CLUE: “PASS”

Answer 10

a. Increase pressure; decrease angle, speed and size
b. Decrease pressure; increase angle, speed and size

Question 11

Characteristics of a good blood smear

Answer 11

Gradual transition from thick to thin
2/3 or 3/4 length
Visible lateral edges
Finger-shaped
Edges with rainbow appearance
With irregularities, holes, streaks, waves, gaps

Question 12

Specimen for blood smear

Answer 12

EDTA- venipuncture
Free flowing- skin puncture

Question 13

Scanning/ counting of cells
a. side to side
b. tail toward the head of the smear
c. back and forth serpentine

Answer 13

a. Cross sectional/ crenellation
b. Longitudinal
c. Battlement/ Track pattern (most preferred method)

Question 14

a polychrome stain containing methylene blue and its products are usually eosin B and Y

Answer 14

Romanowsky stains

Question 15

Examples of romanowsky stain

Answer 15

Wright’s stain
Giemsa stain
Leishman stain
Jenner stain
May Grunwald

Question 16

Color in smear
a. cell nucleus
b. cytoplasm

Answer 16

a. purple
b. blue and pink

Question 17

Fixative that is toxic and causes blindness

Answer 17

Methanol

Question 18

Blood smear buffer

Answer 18

aged distilled water and 0.05M sodium phosphate

Question 19

Blood smear should be stained _______ after collection

Answer 19

2-3 hours

Question 20

a. pH for blood and bone marrow staining
b. pH for malarial parasites

Answer 20

a. pH 6.8
b. pH 7.2

Question 21

color of the following viewed microscopically
a. RBC
b. WBC nuclei
c. cytoplasm of neutrophils
d. eosinophils

Answer 21

a. orange to salmon pink
b. purple to blue
c. pink to tan or iliac granules
d. bright orange or refractile granules

Question 22

Grainy appearance of the film may indicate

Answer 22

RBC agglutination

Question 23

Holes all over the film may indicate

Answer 23

Increase lipid levels

Question 24

Film is bluer overall

Answer 24

Increased blood proteins and rouleaux is present

Question 25

Stain for thick and thin smear for malaria

Answer 25

Giemsa stain

Question 26

Stain that is most common in film preparation

Answer 26

Wright's stain

Question 27

longer smear

Answer 27

thin; decrease angle (vice versa)

Question 28

Positive for feulgen, supravital and wright stain

Answer 28

Howell-Jolly bodies

Question 29

The only positive in feulgen stain

Answer 29

Howell-Jolly bodies

Question 30

Negative in Wright stain

Answer 30

Reticulocytes Heinz Bodies

Question 31

It could be negative nor positive in wright stain

Answer 31

Heinz bodies

Question 32

Positive in supravital stain

Answer 32

basophilic stippling howell-jolly bodies

Question 33

Can be demonstrated with crystal violet stain

Answer 33

Heinz bodies

Question 34

a. Demonstrates the presence of DNA b. Demonstrates the presence of RNA

Answer 34

A. feulgen stain B. Supravital stain

Question 35

Demonstrated in new methylene blue stain

Answer 35

reticulocytes

Question 36

enumerate the RBC inclusions

Answer 36

Basophilic stippling Cabot rings Howell-Jolly bodies Polychromatophilia Reticulocytes Pappenheimer bodies Heinz bodies

Question 37

Overall film quality, color and distribution of cells can be assessed; used for checking fibrin strands

Answer 37

LPO

Question 38

Rouleaux formation and RBC agglutination is easy seen at this objective

Answer 38

LPO

Question 39

Seen in LPO; presence of more than four times the number of cells at the edges or feather compared with monolayer

Answer 39

snowplow effect

Question 40

Selection of the correct area of the film in which to begin

Answer 40

HPO

Question 41

WBC estimate a. HPO (40x) b. OIO (50x) c. OIO (100x)

Answer 41

a. HPO (40x)= average # of WBCs per field x 2000 b. OIO (50x)= average # of WBCs per field x 3000 c. OIO (100x)= average # of platelets per field x 20,000

Question 42

How many RBCs per 100 x oil immersion field are present in normal RBC count?

Answer 42

200-250 RBCs