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HEMATOLOGY > Blood Smear Preparation > Flashcards

Blood Smear Preparation Flashcards

1
Q

Two cover slide smears; used for bone marrow sample

A

Ehrlich

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2
Q

Cover slide and glass slide; used for bone marrow sample

A

Beacom

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3
Q

Most preferred method that uses two glass slide; spread with 35-45 degree angle

A

Wedge smear

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4
Q

glass slide size

A

3x1 inch
(75mm x 25mm x 1-1.2mm thick)

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5
Q

Smear for platelet, SLE and WBC count

A

buffy coat smear (spun smear)

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6
Q

Gold standard for blood parasites (malaria)

A

thick blood smear

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7
Q

semi automatic; stimulates the manual wedge technique

A

Miniprep

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8
Q

a. patient with increased hematocrit
b. angle of spreader

A

a. polycythemia vera
b. lowered to 25 degree angle

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9
Q

a. patient with decreased hematocrit
b. angle of spreader

A

a. anemia
c. higher to 45 degree angle

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10
Q

a. Thin smear
c. Thick smear

CLUE: “PASS”

A

a. Increase pressure; decrease angle, speed and size
b. Decrease pressure; increase angle, speed and size

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11
Q

Characteristics of a good blood smear

A

Gradual transition from thick to thin
2/3 or 3/4 length
Visible lateral edges
Finger-shaped
Edges with rainbow appearance
With irregularities, holes, streaks, waves, gaps

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12
Q

Specimen for blood smear

A

EDTA- venipuncture
Free flowing- skin puncture

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13
Q

Scanning/ counting of cells
a. side to side
b. tail toward the head of the smear
c. back and forth serpentine

A

a. Cross sectional/ crenellation
b. Longitudinal
c. Battlement/ Track pattern (most preferred method)

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14
Q

a polychrome stain containing methylene blue and its products are usually eosin B and Y

A

Romanowsky stains

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15
Q

Examples of romanowsky stain

A

Wright’s stain
Giemsa stain
Leishman stain
Jenner stain
May Grunwald

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16
Q

Color in smear
a. cell nucleus
b. cytoplasm

A

a. purple
b. blue and pink

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17
Q

Fixative that is toxic and causes blindness

A

Methanol

18
Q

Blood smear buffer

A

aged distilled water and 0.05M sodium phosphate

19
Q

Blood smear should be stained _______ after collection

A

2-3 hours

20
Q

a. pH for blood and bone marrow staining
b. pH for malarial parasites

A

a. pH 6.8
b. pH 7.2

21
Q

color of the following viewed microscopically
a. RBC
b. WBC nuclei
c. cytoplasm of neutrophils
d. eosinophils

A

a. orange to salmon pink
b. purple to blue
c. pink to tan or iliac granules
d. bright orange or refractile granules

22
Q

Grainy appearance of the film may indicate

A

RBC agglutination

23
Q

Holes all over the film may indicate

A

Increase lipid levels

24
Q

Film is bluer overall

A

Increased blood proteins and rouleaux is present

25
Q

Stain for thick and thin smear for malaria

A

Giemsa stain

26
Q

Stain that is most common in film preparation

A

Wright’s stain

27
Q

longer smear

A

thin; decrease angle
(vice versa)

28
Q

Positive for feulgen, supravital and wright stain

A

Howell-Jolly bodies

29
Q

The only positive in feulgen stain

A

Howell-Jolly bodies

30
Q

Negative in Wright stain

A

Reticulocytes
Heinz Bodies

31
Q

It could be negative nor positive in wright stain

A

Heinz bodies

32
Q

Positive in supravital stain

A

basophilic stippling
howell-jolly bodies

33
Q

Can be demonstrated with crystal violet stain

A

Heinz bodies

34
Q

a. Demonstrates the presence of DNA
b. Demonstrates the presence of RNA

A

A. feulgen stain
B. Supravital stain

35
Q

Demonstrated in new methylene blue stain

A

reticulocytes

36
Q

enumerate the RBC inclusions

A

Basophilic stippling
Cabot rings
Howell-Jolly bodies
Polychromatophilia
Reticulocytes
Pappenheimer bodies
Heinz bodies

37
Q

Overall film quality, color and distribution of cells can be assessed; used for checking fibrin strands

A

LPO

38
Q

Rouleaux formation and RBC agglutination is easy seen at this objective

A

LPO

39
Q

Seen in LPO; presence of more than four times the number of cells at the edges or feather compared with monolayer

A

snowplow effect

40
Q

Selection of the correct area of the film in which to begin

A

HPO

41
Q

WBC estimate
a. HPO (40x)
b. OIO (50x)
c. OIO (100x)

A

a. HPO (40x)= average # of WBCs per field x 2000
b. OIO (50x)= average # of WBCs per field x 3000
c. OIO (100x)= average # of platelets per field x 20,000

42
Q

How many RBCs per 100 x oil immersion field are present in normal RBC count?

A

200-250 RBCs

HEMATOLOGY (9 decks)

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