Blood Smears Flashcards

1
Q

peripheral smear

A

info regarding BM function

assess WBC, RBC, platelets

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2
Q

how to prepare peripheral smear

A

two slides

drop of blood on one slide
-second slide to spread across slide

blood goes from thick to thin

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3
Q

size of RBC

A

6-8 microns diameter
2 microns thick

biconcave disc

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4
Q

number of RBCs

A

normal - 4-6 x 10/6

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5
Q

lymphocyte size

A

6 microns

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6
Q

anisocytosis

A

variation in size of cells that are normally uniform

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7
Q

reticulocyte

A

no central pallor and larger than typical RBC

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8
Q

hypersegmented neutrophils

A

with macrocytic anemia**

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9
Q

poikilocytosis

A

variation in RBC shape

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10
Q

schistocytes

A

fragmented RBCs

TTP, DIC, HUS, defective heart valve, hemolytic anemias

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11
Q

acanthocyte

A

burr cell

projections

liver disorders

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12
Q

codocyte

A

target cell

sickle cell, HbC, thalassemias

do a Hg electrophoresis**

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13
Q

dacrocyte

A

tear drop

myeloproliferative disorder, myelofibrosis, pernicious anemia, thalassemia

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14
Q

stomatocyte

A

folded RBC - looks like mouth and lips

hemolytic anemias - constitutive or acquired

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15
Q

burr cell

A

acanthocyte

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16
Q

target cell

A

codocyte

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17
Q

tear drop

A

dacrocyte

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18
Q

poikilocytosis and anisocytosis

A

sickle cell anemia

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19
Q

poikilocytosis and anisocytosis with microcytes

A

beta-thal

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20
Q

sickledex solubility test

A

for SS trait and disease

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21
Q

sickle cell trait

A

HbS and HbA

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22
Q

sickle cell anemia

A

HbS and HbS

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23
Q

Hg in RBC

A

270 million Hg in one RBC - is the max

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24
Q

hypochromic

A

decreased [Hg]

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25
central pallor or RBC
should be 1/3 of cell
26
increased central pallor
thalassemia Fe deficiency sideroblastic
27
decreased central pallor
spherocytosis | autoimmune hemolysis
28
recent blood transfusion
can see normal cells on peripheral smear
29
stack of coins
rouleaux formation multiple myeloma elevated plasma fibrinogen or globulins - increased proteins
30
clumping
cold agglutinins
31
normoblast
nucleated RBC indicate stressed bone marrow unable to meet increased RBC requirements hemolytic crises
32
basophilic stippling
dark-blue granules in RBC lead poisoning
33
lead poisoning
basophilic stippling
34
howell jolly bodies
blue-black RBC inclusions on wright stained smear nuclear fragments hemolytic anemias or post-splenectomy patients
35
signet ring
plasmodium vivax malaria blood smear
36
elliptocytes
hereditary
37
total WBC range
4-11 x 10/3
38
differential
for WBC differntiates 5 main types of WBCs - gives percentage
39
absolute count
take total count times percent of cell type
40
nuetrophils
50-60% bacterial infections, stress, corticosteroid therapy**
41
corticosteroid therapy
can cause large increase in neutrophils
42
lymphocytes
30-40% elevated in viral infections
43
atypical lymphocytes
mononucleosis EBV
44
fragile lymphocytes
smudge or basket cells in CLL
45
monocytes
4%
46
eosinophils
<5% parasite and allergy
47
basophils
<1% elevated in CML
48
left shift
increase in immature forms in peripheral blood due to increased production -infection, myeloid cancers
49
band cell
immature neutrophil
50
dohle body
toxic graunlation in cytoplasmc vacuole of WBC systemic infection or inflammatory disease
51
hypersegmented neutrophils
>5 lobes megaloblastic anemia
52
reduced lobulation of mature neutrophils
peger-huet anomaly myelodysplastic syndromes
53
blasts WBC
bad myeloid or lymphoid neoplasia of WBCs
54
plasma cells
lymphoid neoplasia multiple myeloma**
55
platelet count
150-450,000
56
megakaryocyte
birth platelets
57
oil immersion field platelets
see 7-20
58
platelets 50,000
time to transfuse 25,000 - spontaneous bleeding
59
platelets 1 million
can start clotting
60
1 unit of platelets
bump you up 25,000 usually give 5 or more**
61
giant platelets
with increased platelet destruction or consumption
62
petechiae
small pinpoint hemorrhage thrombocytopenia
63
purpura
larger skin hemorrhage thrombocytopenia concerned with internal bleeding
64
dohle bodies
sepsis
65
eosinophils
parasitic infection check stool for ova from parasites
66
target cell
congeintal abnormalities thalassemia
67
schistocytes
fragmented RBCs hemolytic anemia
68
band neutrophils
systemic infection