Biotechnology Flashcards
Recombinant DNA
Made from combining pieces of DNA from different sources
Why is a bacteria host usually used
Fast reproduction and presence of plasmid
Transgenic organism
Contain foreign DNA
Cleavage
Cut out desired piece of DNA using enzymes
Splicing
Attach the DNA to a vehicle
Adding a piece of DNA
Insertion
Insert the DNA into a host cell
Cloning
Producing genetically identical copies of cell, tissue, organism
GMO crops
Crops that have been genetically modified to have specific traits
GMO
Frameshift mutation of a DNA sequence that doesn’t change naturally
Stem cells
Basic cells that can become any type of cell
Types of stem cells
Adult and embryo cells
IVF and PGT
PGT ensures the embryo a safe transfer with the correct amount of chromosomes
Human genome project
International study done to map and sequence all 46 human chromosomes
Gene therapy
Transplant of normal genes into cells in place of missing/defective ones in order to correct the disorder
Bioremediation
Using naturally occurring/introduced microorganisms to the environment
Good
First cloned organism
Dolly the sheep
How many genes per chromosomes
80,000 per 46 chromosomes
Amniocentesis
To check on embryo using amniotic fluids
Gel electrophoresis
One way to create a DNA finger print
Restriction enzymes
Cut the DNA into pieces
Electrical current pushes
More of the smaller pieces of DNA to the positive side than larger ones
Children get
Half of their chromosomes from each parent