Biosynthesis of fatty acids

Question 1

Difference between beta oxidation and synthesis

Answer 1

Oxidation/ reduction reagents are for synthesis are: NADP+/NADPH
For beta oxidation NAD+/NADH FAD+/FADH

Question 2

Difference between beta oxidation and synthesis

Answer 2

Synthesis: Enzymes are contained in one long polypeptide chain
Beta oxidation: enzymes are independent enzymes found in the matrix

Question 3

Difference between beta oxidation and synthesis of fatty acid?

Answer 3

Biosynthesis occurs in cytosol
Beta oxidation occurs in mitochondria

Intermediate of FA synthesis are linked to ACP
Beta oxidation is linked to COA

Question 4

What are fatty acids?

Answer 4

Long chain hydrocarbon molecules containing a carboxylic acid moiety at one end

Question 5

The Acyl group is anchored tona protein group by a ……….. linkage

Answer 5

Thioester

Question 6

Acyl group of B oxidation and FA synthesis are anchored to …….. and ……..respectively

Answer 6

CoA
ACP

Question 7

ACP and COA are similar in ………… But differ in ………..

Answer 7

Similar, in that they both contain phosphopantetheine group
Differ because that of COA is linked to Adenosine nucleotide

ACP is linked to a serine residue

Question 8

What enzyme cleaves palmitate from ACP cleaving the thioester bond

Answer 8

Palmitoyl Thioesterase