Biology Quiz 2 Experiment questions Flashcards
What is the overall goal of the GFP project
to take the GFP gene that has been “cloned” from jellyfish and transform it into bacteria
What does GFP stand for?
green fluorescent protein
What organism does the GFP gene come from?
jellyfish
What is the structure of the GFP gene?
barrell composed of 11 beta sheets and six alpha helices
In the cell where does protein come from?
expressed from a gene
Where are genes found in the cell?
nucleus
Where are genes located in the nucleus?
part of the chromosomes
What are genes made up of?
DNA
How many genes do humans have
approximately 20,000
What does it mean that genes can be cloned?
genes can be isolated from a source organism and placed into another organism
What type of bacteria will be putting GFP in?
E. coli
What are the two techniques we will be using to analyze DNA?
Restriction enzymes
Gel electrophoresis
What is the process of using restriction enzymes to analyze the DNA?
enzymes that cut DNA at specific sequences of bases
What are the sequences where the enzymes cut?
restriction sites
What is the process of using Gel electrphoresis to analyze DNA
separate DNA fragments based on size
