Biology Quiz 1 Flashcards
What are the two types of microscopes?
Electron Microscope
Light Microscope
What can be seen in the light microscope?
Can observe single cells
Cell Membrane
Nucleus
Cytoplasm
What can be observed through electron microscope?
Can observe cellular organelles like:
Golgi
ER
Mitochondria
Why does electron microscope have higher resolution than light?
Shorter Wavelength that allows smaller objects to be observed
(the shorter the wavelength the better the image)
What are the two types of Electron Microscopes?
Scanning
Transmission
What does a scanning electron microscope observe and what type of picture is given?
The surface (3-D)
What does a transmission electron microscope observe and what type of picture is given?
Internal “parts” organelles (2-D)
How does Transmission EM view and display image? (what is used ex light in light microscope)
A series of lenses pass an electron beam through specimen and show 2-D image
How does Fluorescent Microscope work?
Uses fluorescent antibodies or dyes to observe specific parts of the cell
*Know anatomy of light microscope
KNOW IT
What is the magnification of the optical lens (eye piece)
10X
What are the objectives lenses and what size?
Lenses are the lenses viewed through sizes are 4, 10, 45, 100
What is the total magnification of the objective and eye piece
40X, 100X, 450X, 1000X
What is the body tube?
Long tube that houses eye piece
What is the nose piece?
Attachment point for the objectives
What is the Iris type diaphragm?
Control the diameter of the condenser and amount of light
What is the condenser?
converges the light rays into a focus beneath object
What is the illuminator?
Provides a light source to show through the object (trans illuminate)
What is the Base?
Bottom of the microscope
What is the fine adjustment Knob
Fine adjustment of focus
What is the coarse adjustment knob
coarse adjustment of focus
What is the stage?
platform on which the object is transilluminated
What is the slide clip?
Hold the slide in place during observation
What is the object?
Specimen under observation
What is the objective?
one of four separate lenses which magnify the image to varying degrees
What does parfocal mean?
Focus one and switch to any one of the others and it will stay in focus
What is the arm?
the bent part of the microscope which holds the lens system above the object.
What is the pointer?
Thin strip of metal located in the body tube. It appears in the field and is useful to point
What is the eyepiece?
a second lens usually 10X
What is the pointer width for 40X magnification
160 um
What is the pointer width for 100X magnification?
65 um
What is the pointer width for 450X magnification?
15 um
What are muscles responsible for?
Body Movement
Organ motion (heart beat)
Heat (ATP)
What shape do muscle cells often have?
oblong cylindrical shape sometimes called “fibers”
What are the 3 main types of muscle tissue and where are they found?
Skeletal (attached to bones)
Cardiac (heart)
Smooth (surround body tubes)
How long are muscle cells?
As long as the muscle