Biochemical Test in Identifying Bacteria Flashcards
a tentative identification
Clinical signs of the disease
definitive identification
Laboratory examination
4 different types of culturing medium
- differential media
- selective media
- enriched media
- enrichment broth
7 types of biochemical test
- catalase test
2 coagulase test - oxidase test
- sugar fermentation test
- indole test
- citrate test
- urease test
3 types of serological test
- slide agglutination test
- tube agglutination test
- microscopic agglutination test
2 types of molecular technique
- PCR
- Sequencing
6 types of microscopy
- wet mount microscopy
- gram staining
- acid-fast staining
- negative staining
- spore staining
- motility staining
Essential in differentiating catalase + Micrococcaceae from catalase - Streptococcaceae
Catalase test
Differentiate strains of Staphylococcus aureus from other coagulase- negative species.
Coagulase test
are capable of coagulating plasma and produces clumps of cells in the slide test.
S.aureus
what catalase test has enzyme ?
positive
Facilitates the detection of the enzyme catalase in bacteria.
Catalase test
what catalase test do not have enzyme ?
negative
what does it mean if the coagulase test has clot formation?
Positive
what does it mean if the coagulase test did not have clot formation?
Negative
4 method variations to the oxidase test
1) filter paper test, (2) filter paper spot test, (3) direct plate method, (4) test tube method
Assays for the presence of cytochrome oxidase, an enzyme sometimes
indophenol oxidase
what does development of deep purple color within 10 seconds in oxidase test?
positive
is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain
oxidase test
Screens for the ability of an organism to degrade the amindo acid tryptophan and produce
Indole Test
a positive diagnostic test rests on the generation of alkaline by products of citrate metabolism
citrate utilization test
screens a bacterial isolate for the ability to utilize citrate as its carbon and energy source
citrate utilization test
the subsequent increase in the pH of the medium is demonstrated by the color change of a pH indicator
citrate utilization test
what is the color for a negative citrate utilization test ?
green
