Biochemical Test in Identifying Bacteria

Question 1

a tentative identification

Answer 1

Clinical signs of the disease

Question 2

definitive identification

Answer 2

Laboratory examination

Question 3

4 different types of culturing medium

Answer 3

1. differential media
2. selective media
3. enriched media
4. enrichment broth

Question 4

7 types of biochemical test

Answer 4

1. catalase test
   2 coagulase test
2. oxidase test
3. sugar fermentation test
4. indole test
5. citrate test
6. urease test

Question 5

3 types of serological test

Answer 5

1. slide agglutination test
2. tube agglutination test
3. microscopic agglutination test

Question 6

2 types of molecular technique

Answer 6

1. PCR
2. Sequencing

Question 7

6 types of microscopy

Answer 7

1. wet mount microscopy
2. gram staining
3. acid-fast staining
4. negative staining
5. spore staining
6. motility staining

Question 7

Essential in differentiating catalase + Micrococcaceae from catalase - Streptococcaceae

Answer 7

Catalase test

Question 8

Differentiate strains of Staphylococcus aureus from other coagulase- negative species.

Answer 8

Coagulase test

Question 8

are capable of coagulating plasma and produces clumps of cells in the slide test.

Answer 8

S.aureus

Question 8

what catalase test has enzyme ?

Answer 8

positive

Question 9

Facilitates the detection of the enzyme catalase in bacteria.

Answer 9

Catalase test

Question 10

what catalase test do not have enzyme ?

Answer 10

negative

Question 11

what does it mean if the coagulase test has clot formation?

Answer 11

Positive

Question 12

what does it mean if the coagulase test did not have clot formation?

Answer 12

Negative

Question 13

4 method variations to the oxidase test

Answer 13

1) filter paper test, (2) filter paper spot test, (3) direct plate method, (4) test tube method

Question 13

Assays for the presence of cytochrome oxidase, an enzyme sometimes

Answer 13

indophenol oxidase

Question 14

what does development of deep purple color within 10 seconds in oxidase test?

Answer 14

positive

Question 15

is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain

Answer 15

oxidase test

Question 16

Screens for the ability of an organism to degrade the amindo acid tryptophan and produce

Answer 16

Indole Test

Question 17

a positive diagnostic test rests on the generation of alkaline by products of citrate metabolism

Answer 17

citrate utilization test

Question 17

screens a bacterial isolate for the ability to utilize citrate as its carbon and energy source

Answer 17

citrate utilization test

Question 18

the subsequent increase in the pH of the medium is demonstrated by the color change of a pH indicator

Answer 18

citrate utilization test

Question 19

what is the color for a negative citrate utilization test ?

Answer 19

green

Question 20

what is the color for a positive citrate utilization test ?

Answer 20

blue

Question 21

what is the color for a negative urease test?

Answer 21

yellow or orange

Question 22

what is the color for a positive urease test?

Answer 22

pink or red violet

Question 23

what is the ingredient for urease test?

Answer 23

Christensen’s Urea Agar

Question 23

identifies those organism that are capable of hydrolyzing urea to produce ammonia and carbon dioxide.

Answer 23

Urease test