Biochemical Engineering Flashcards
Bond between monosaccharides
glycosidic bonds
Primary level of protein structure
amino acid sequence
Secondary level of protein sturcture
Alpha helix coil or beta sheet
Tertiary protein structure
fold of proteins
Quaternary structure of proteins
Polypeptide chains
Protein chains that are easily soluble in water, found in enzymes, spherical conformation
Globular
Protein chains that are found as parallel polypeptide chains in long sheets, water insoluble, tough, stretchy
Fibrous
Types of inhibition
Competitive, non-competitive
Inhibition type where the binding site is blocked
Competitive
Inhibition type that changes the shape of enzyme
non-competitive
Generally-accepted model for enzyme fit
Induced fit model
Lengths of proteins
> 50 amino acids
Lengths of peptides
< 50 amino acids
Bonds that link amino acids together
peptide bonds
The only secondary amine out of the 20 amino acids
proline
the only achiral amino acid
glycine
Natural configuration of amino acids
L
pH where the amino acid exists as neutral, zwitterionic form
Isoelectric point
Separation of polar compounds based on mobility (charge and molecular mass)
electrophoresis
Convention of writing peptide sequences
N-terminus to C-terminus
Enzyme that catalyzes hydrolysis of peptide bonds
Proteases
Organic molecules/metal ions that aid enzymes in reactions
coenzymes
When the carbonyl of a monosaccharide is located at the C1 carbon
aldose
When the carbonyl of a monosaccharide is located at any carbon except C1
ketose
Carbohydrates with the OH group pointing to the right of the Fischer projection
D-carbohydrates
The simplest carbohydrate
glyceraldehyde
Process of forming an equilibrium mixture of the anomers
mutarotation
Carbohydrate with a missing hydroxyl group
Deoxy sugar
Carbohydrates with hydroxyl group replaced by amino group
amino sugar
Long, straight chain saturated/unsat’d acids (C12-C20)
Fatty acids
tri-esters formed from glycerol and fatty acids
triglycerides
sodium and potassium salts of fatty acids produced from the hydrolysis of glycerides
soaps
component of cell membranes with nonpolar tails and polar heads
glycerophospholipids
esters of long chain fatty acids (C16 - C36) with long chain alcohols
waxes
Biological process of breaking down molecules
catabolism
Biological process of building molecules
Anabolism
Net product of cellular respiration in # of ATP
36 or 38
Product of glycolysis
2 ATP + 2 pyruvates
Product of citric acid cycle
2 ATP (due to 2 citric acid processed)
Product of electron transport chain
32-34 ATP per glucose
Fermentation product of pyruvate in animals
lactate
Net product of fermentation
2 ATP per glucose
Product of fermentation in plants
alcohol and CO2
Precursor of glucose for gluconeogenesis
pyruvates
Efficiency of cellular respiration
39% (263 kcal/686 kcal)
three subunits of nucleotides
sugar, heterocyclic base, phosphodiester
base sugar of DNA
2-deoxyribose
base sugar of RNA
ribose
Composed of only a sugar and base
Nucleoside
Composed of a sugar, base, and phosphate
Nucleotide
Chain of sugars and bases attached via phosphodiesters
nucleic acids
Linkages between nucleotide units of nucleic acids
phosphodiesters
Describe Chargaff’s Rule
A = T, C = G
Secondary structure of DNA
double helix
Tertiary structure of DNA
supercoils
assembly of DNA coiled around protein cores called histones
nucleosomes
process of copying DNA
replication
process of reading and transferring genetic code from the DNA to mRNA
transcription
process of converting genetic code into a protein
translation
enzyme that unwinds DNA
topoisomerase
protein that unwinds the double helix DNA
helicase
enzyme that replicates DNA to produce a new strand
DNA polymerase
enzyme that forms the phosphodiester bond between DNA pieces
DNA ligase
describe the replication in the leading strand
proceeds as usual in the 5’ –> 3’ direction of the new strand
describe the replication of the lagging strand
synthesis of individual 5’ –> 3’ fragments are done and fused together using DNA ligase
strand that is copied during transcription
antisense strand
large assembly of protein and rRNA that catalyze protein and peptide biosynthesis
ribosomes
method of amplifying DNA using polymerase, dNTPs, and temperature cycling
polymerase chain reaction (PCR)
unhealthy types of fats
saturated and trans fats
purine bases
A/G
Pyrimidine bases
C/U/T
stop codon
AUG