Bio Test 2 Flashcards

1
Q

Prokaryote

A

Unicellular organisms have this type of cell. Some features include:
- Not having a nucleus
- Lacks organelles, organization
- Smaller than eukaryotes

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2
Q

Eukaryote

A

Multicellular organisms have this type of cell. Some features include:
- Having a nucleus
- Having organelles
- Larger than prokaryotes

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3
Q

Cilium (Cilia)

A
  • Present in eukaryotes only
  • Functions to either move the cell itself or move things outside the cell (in the environment)
  • Tiny hair-like threads on the surface of the cell (but still considered membrane-bound)
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4
Q

Flagellum (Flagella)

A
  • Present in both prokaryotes and eukaryotes (sperm cell is the only flagellum in the human body)
  • Motile
  • Thread-like tail structure
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5
Q

Osmotic Bursting / Cytolysis / Osmotic Lysis

A

When the cell bursts due to an osmotic imbalance. The cell gains too much water through diffusion and ends up rupturing. Cell wall prevents this phenomenon.

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6
Q

Cell Wall

A
  • Found in some prokaryotes and some eukaryotes (plants)
  • Mainly made of cellulose
  • Covers the cell membrane
  • Provides protection; tough surface
  • Prevents osmotic bursting
  • Fully permeable
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7
Q

Pilus (Pili)

A
  • Found only in prokaryotes
  • Functions to help cells with adhesion or attachment to other cells
  • Thin and hair-like structure, smaller than a flagellum
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8
Q

Nucleoid

A

Region in a prokaryote where DNA is found

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9
Q

Glycocalix / Capsule / Slime Layer

A
  • Sticky outer layer of a prokaryote
  • Glycocalix means “sugar coat”
  • Made of polysaccharides and proteins
  • Helps bacteria evade immune system more easily
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10
Q

Biofilm

A

Groups of bacteria sticking together, creating slimy coatings to protect them

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11
Q

3 Domains of Life are…

A
  1. Archaea (prokaryotic)
  2. Bacteria (prokaryotic)
  3. Eukarya (eukaryotic)
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12
Q

Gram-positive

A

Thick cell wall:
- Thick peptidoglycan layer
- No outer membrane
- Retains color in gram staining

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13
Q

Gram-negative

A

Thin cell wall:
- Thin peptidoglycan layer
- Has an outer membrane (lipopolysaccharides)
- Does not retain color in gram staining

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14
Q

Gram Staining

A

Involves crystal violet dye, iodine solution, and ethyl alcohol or acetone.
- Crystal violet dye + iodine solution form crystal violet-iodine complex
- Ethyl alcohol or acetone dehydrates peptidoglycan layer, causing it to shrink
- In gram-positive bacteria, the violet-idoine complex cannot penetrate the shrunken peptidoglycan layer, causing it to have a purple color
- In gram-negative bacteria, the peptidoglycan layer is too thin to hold the complex crystals, causing color to be lost

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15
Q

Differential Staining

A

A technique where you use different dyes to stain different microorganisms on a slide, allowing you to differentiate between the two of them more easily

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16
Q

Lipopolysaccharide (LPs)

A
  • Part of the outer membrane in gram-negative bacteria (and the outer membrane is part of the cell wall)
  • Consists of a lipid and polysaccharide
  • Endotoxin, harms the human body
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17
Q

Peptidoglycan

A
  • Part of the cell wall in bacteria
  • Thicker layers in gram-positive bacteria
  • Thinner layers in gram-negative bacteria
  • Made of, as the name implies, glycan (anything with glycosidic linkages) chains and short peptides
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18
Q

Nucleus

A
  • Where DNA is stored
  • Contains the nucleolus
  • Contains chromatin
  • Has a nuclear membrane made of two layers
  • Has nucleus pores
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19
Q

Nuclear DNA

A
  • Term used to describe DNA found in the nucleus of a cell
  • Both paternal and maternal DNA
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20
Q

Histones

A

Proteins in chromatin that help give structure to chromosomes (made of DNA)

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21
Q

Chromatin

A

Histones + DNA

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22
Q

Nucleolus

A
  • Transcribes/Produces rRNA
  • Produces ribosomes
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23
Q

Nuclear Membrane / Nuclear Envelope

A
  • Made of two phospholipid bilayers (inner and outer)
  • Semi-permeable
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24
Q

Nucleus Pores

A
  • Holes in the nuclear membrane
  • Allows select molecules to pass (such as RNA)
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25
Q

Rough Endoplasmic Reticulum

A
  • Site of protein synthesis (produces proteins)
  • Contains ribosomes
  • Makes membrane
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26
Q

Ribosomes

A
  • Functions to actually synthesize and produce proteins
  • Found in the rough ER
  • Made in the nucleolus
  • Has a large subunit and small subunit
  • Made of rRNA and protein
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27
Q

Smooth Endoplasmic Reticulum

A
  • Synthesizes lipids
  • Metabolizes carbohydrates
  • Detoxification of drugs and poisons
  • Makes vesicles (membranes of phospholipid bilayer + proteins)
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28
Q

Golgi Apparatus

A
  • Modifies lipids and proteins
  • Sends them to destinations in vesicles
  • Produces vesicles just like the smooth ER does
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29
Q

DNA Cycle

A

DNA → mRNA → out of nuclear pores → rough ER → ribosome → protein → smooth ER → vesicle → golgi apparatus → vesicle → cell membrane

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30
Q

Mitochondrion (Mitochondria)

A
  • Found in eukaryotic cells
  • Produces ATP via cellular respiration
  • Has its own ribosomes (can make proteins)
  • Has its own DNA
  • Mitochondrial DNA is maternal only
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31
Q

Lysosome

A
  • ONLY for animal cells, NOT in plant cells
  • Has hydrolytic enzymes (enzymes involved in hydrolysis)
  • Involved in breaking down worn-out or excess cell parts
  • Can also destroy viruses or bacteria
  • “Clean up” organelle
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32
Q

Central Vacuole

A
  • ONLY for plant cells and fungi cells, NOT in animal cells
  • Stores chemicals for later use
  • Stores water to provide rigidity
  • Stores macromolecules
  • Storage organelle in plants
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33
Q

Chloroplast

A
  • Only in plants
  • Converts CO2 and H2O into sugar using light energy
  • Has its own ribosomes
  • Has its own DNA
  • Made of thylakoids (in grana) and stroma
  • Contains chlorophyll in grana
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34
Q

Thylakoid

A
  • Flattened sacs in chloroplasts
  • Photosynthesis takes place here
  • Stacks of thylakoids are called grana (singular: granum)
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35
Q

Granum (Grana)

A

Stacks of thylakoids

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36
Q

Stroma

A
  • Colorless fluid around the grana in chloroplasts
  • Photosynthesis is started here and finished in thylakoids
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37
Q

Centrosome

A
  • Only found in animal cells
  • Gives structure to cell
  • Primary microtubule-organizing center (MTOC), moves microtubules around, aka shifts cytoskeleton
  • “Funny” vacuole
  • Contains centrioles
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38
Q

Centriole/Centrosome

A
  • Produces spindle fibers used to move chromosomes around the cell during mitosis (centriole)
  • Involved in flagella and cilia formation (centrosome)
  • Helps determine locations of organelles in the cell (centrosome)
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39
Q

Cytoskeleton

A
  • Gives cell its structure
  • Consists of 3 components: microtubules, microfilaments, and intermediary filaments
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40
Q

Endomembrane System

A
  • How membrane-bound organelles interact
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41
Q

Fluid Mosaic

A

The idea that the plasma membrane is made of a mix of lipids, proteins, and carbohydrates

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42
Q

Plasma Membrane

A
  • Aka cell membrane
  • Phospholipid bilayer
  • Selectively permeable, allows only specific molecules to pass
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43
Q

Membrane Fluidity

A
  • Viscous: saturated hydrocarbon tails, straight
  • Fluid: unsaturated hydrocarbon tails, kinked
  • Cholesterol can fill in, making saturated tails more fluid and unsaturated tails more viscous (good balance)
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44
Q

Glycoprotein

A
  • Protein attached to oligosaccharide
  • Modified protein
  • Involved in cell-to-cell recognition, virus-to-cell recognition, bacteria-to-cell recognition
  • On the outside of a plasma membrane with the oligosaccharde facing out
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45
Q

Glycolipid

A
  • Lipid attached to oligosaccharide
  • Modified lipid
  • Involved in cell-to-cell recognition, virus-to-cell recognition, bacteria-to-cell recognition
  • On the outside of a plasma membrane with the oligosaccharde facing out
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46
Q

Phospholipid Movement

A
  • Flips laterally (horizontally) about 10 million times per second
  • Flip-flops or switches vertically about once per month
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47
Q

Simple Diffusion

A
  • Passive transport
  • Involves either hydrophobic molecules or small, uncharged, and polar molecules
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48
Q

Facilitated Diffusion

A
  • Passive transport
  • Involves either ions or large and polar molecules (even if uncharged)
49
Q

Transmembrane Protein

A
  • Spans the whole membrane
  • Nonpolar amino acids interact with the phospholipid bilayer
  • Polar amino acids face outside and make a kind of channel to facilitate diffusion of polar molecules
  • Often coiled in alpha helixes, helical bundles, or beta barrels
50
Q

Membrane Proteins help with…

A
  • Intercellular joining
  • Enzymatic activity
  • Transport (active or passive)
  • Cell-to-cell recognition (glycoproteins)
  • Signal transduction
51
Q

Active Transport

A
  • Uses energy from ATP to transport molecules
  • Against the gradient
  • Carrier changes shape
  • Produces a concentration gradient
52
Q

Passive Transport

A
  • Does not use ATP energy
  • Small molecules can passively diffuse through membranes
  • With the gradient
  • Larger molecules and ions enter through proteins (channel proteins or passive carrier proteins)
  • Diffusion is ALWAYS passive
53
Q

Aquaporin

A

Channel protein that allows water to pass

54
Q

Gradient

A
  • Low concentration on one side and high concentration on the other
  • A form of potential energy (PE)
55
Q

Glute

A

Carrier protein that allows glucose to pass (passive transport)

56
Q

ATP Hydrolysis

A

Breaks down ATP into ADP and inorganic phosphate (Pi), also ATPase activity

57
Q

Phosphorylation

A

Pi binds to a molecule and changes its shape; verb is ‘phosphorilate’

58
Q

Conformational Change

A

Shape change of a protein due to phosphorilation; changes the protein’s function due to the change in shape

59
Q

Dephosphorylation

A

Loses the inorganic phosphate that bonded to the molecule

60
Q

Na+/K+ pump (Sodium-Potassium ATPase or pump)

A

Involves ATP hydrolysis, phosphorilation, conformational change, dephosphorilation to actively transpor Na+ and K+ ions

61
Q

Proton Pump + Sucrose-H+ Cotransporter

A
  • Proton pump uses ATP to transport protons (H+) against the gradient
  • Protons eventually diffuse back into the cell but also cotransport sucrose, which can be used to create ATP
62
Q

Bulk Transport

A
  • For large molecules to pass
  • Requires ATP
  • Includes exocytosis and endocytosis
  • Also for cells “eating” (engulfing) other cells
63
Q

Exocytosis

A

Transport large molecules out of the cell

64
Q

Endocytosis

A

Transport large molecules into the cell

65
Q

Pinocytosis

A

Cell eats droplets of extracellular fluid with molecules in it, nonspecific process, endocytosis

66
Q

Phagocytosis

A

Cell eats bacteria or another cell

67
Q

Macrophage

A

“Big eater” cell eating

68
Q

Antibodies

A

Cause viruses to become sluggish and easier to destroy

69
Q

Pseudopod / Pseudopodium

A

Temporary extensions of the cytoplasm to wrap around the bacteria, cell, or molecule and engulf it

70
Q

Amoeba

A

A single eukaryotic cell

71
Q

Protozoa

A

A type of parasite

72
Q

Receptor-mediated Endocytosis

A

Binds to a receptor and then pseudopod engulfs it

73
Q

Low-density Lipoprotein (LDL) Cholesterol

A
  • “Bad” cholesterol, can lead to build-ups
  • Cholesterol surrounded by proteins and phospholipid coat
74
Q

Metabolism

A

Totality of an organism’s chemical reactions

75
Q

Catabolic Reaction

A

Reaction that involves breaking things down (like hydrolysis)

76
Q

Anabolic Reaction

A

Reaction that involves building/synthesizing things (like dehydration)

77
Q

Metabolic pathways

A
  • Can be linear or cyclic or branched
  • Pathways for chemical reactions
78
Q

Nucleoside

A

A nucleotide without any phosphate

79
Q

Distal or Terminal Phosphate Group

A

The last, most distant phosphate group from the rest of the structure

80
Q

First Law of Thermodynamics

A

Energy cannot be created or destroyed (same with matter)

81
Q

Second Law of Thermodynamics

A

During energy transfer, energy is lost to the environment as heat

82
Q

Exergonic

A
  • Spontaneous
  • Passive
  • Decrease in energy
83
Q

Endergonic

A
  • Nonspontaneous
  • Active
  • Requires energy
84
Q

Energy Gradient

A

Difference in energy on different sides of a membrane

85
Q

Coupling

A

When energy is released during a spontaneous process and the energy released is used to allow a nonspontaneous reaction to occur

86
Q

ATP Gibbs Free Energy

A

-7 kcal/mol

87
Q

What is the final acceptor on the ETC?

A

Oxygen due to electronegativity

88
Q

Ribosimes

A

Ribosomal Enzymes

89
Q

Hexokinase

A

Kinase adds phosphate group, hexokinase adds phosphates to 6-carbon sugars

90
Q

Anabolic Enzyme

A

Enzyme involved in synthesis

91
Q

Two models for enzymes

A
  • Lock-and-key model (self, explanatory)
  • Induced-fit model (fits around the enzyme, not like a lock and key)
92
Q

Cofactor

A
  • Non-protein
  • Inorganic compound
  • Neutral ion
  • Divalent (could make a +2 charge like Mg, Ca, Mn)
  • Enzyme helper
93
Q

Coenzyme

A
  • Small organic molecules
  • Enzyme helper
94
Q

Apoenzyme

A

Inactive enzyme

95
Q

Holoenzyme

A

Active enzyme (w/ helper)

96
Q

What is the optimal temperature for human enzymes?

A

98.6°F or 37°C

97
Q

Thermus Aquaticus

A

Hot water (70 degrees Celsius)
Bacteria found in hot springs and is eally usful dfodf somdkj

98
Q

Acidophile

A

Likes acidic environments

99
Q

Neutrophile

A

Likes neutral (pH-wise) environments

100
Q

Inhibitor

A
  • Interferes w/ ES complex
  • Competitive (competes directly) vs Noncompetitive (changes the shape of the enzyme)
  • Reversible vs Irreversible
101
Q

Allosteric Control

A
  • Allosteric Activator (locks protein in active state, bonds elswhere to activate)
  • Allosteric Inhibitor (locks protein in inactive form, bonds elsewhere to inhibit)
102
Q

Cooperativity

A
  • Is a type of allosteric control
  • Substrate causes subunit to activate, which then causes the next subunit to activate and so on
103
Q

Feedback Inhibition

A

Enzyme’s end product binds to enzyme as an allosteric inhibitor to prevent more product from forming (prevent enzyme from activating and working)

104
Q

Cellular Respiration Equation

A

C₆H₁₂O₆ + 6O₂ → 6CO₂ + 6H₂O + ATP + Heat

105
Q

Electron Carrier

A
  • Carries Electrons to the Electron Transport Chain (Oxidative Phosphorilation)
  • NADH, FADH₂ (reducing agents too)
106
Q

3 Stages of Cellular Respiration

A
  1. Glycolysis
  2. Citric Acid Cycle / KREBS Cycle / Tricarboxylic Acid Cycle (TCA Cycle)
  3. Oxidative Phosphorilation / Electron Transport Chain (ETC)
107
Q

Substrate-level Phosphorylation

A

Transfer of phosphate from a substrate to ADP molecule to generate ATP

108
Q

Step 1 of Glycolysis

A
  • Begins w/ glucose
  • Enzyme hexokinase involved
  • Add phosphate group
  • Produces glucose 6-phosphate
  • Committed/Irreversible step (uses ATP)
109
Q

Step 2 of Glycolysis

A
  • Starts w/ glucose 6-phosphate
  • Enzyme phosphoglucose isomerase involved
  • Produces fructose 6-phosphate
  • Noncommitted/Reversible step
110
Q

Step 3 of Glycolysis

A
  • Begins w/ fructose 6-phosphate
  • Uses pfk or phosphofructokinase
  • Adds phosphate group
  • Produces fructose 1, 6-biphosphate
  • Committed/Irreversible step (uses ATP)
111
Q

Glycolysis (what to know overall)

A
  • Starts w/ glucose
  • Ends w/ pyruvate
  • Pyruvate gets converted into something else (CoA) to go to the TCA cycle
  • Net x2 ATP
  • Makes NADH as well
  • Aerobic
112
Q

KREBS Cycle

A
  • CO₂ comes off
  • NADH produced
  • x2 ATP
  • FADH₂ produced
  • Many intermediates
  • Aerobic
113
Q

ETC

A
  • Electron transport chain
  • Redox!!
  • H⁺ protons cross the membrane when redox reactions occur
  • Aerobic
  • ATP synthase allows H⁺ protons to pass the innermitochondrial membrane into the mitochondrial matrix
  • Chemiosmosis occurs - diffusion of H⁺ ions across ATP synthase, also produces ATP
  • ATP synthase and chemiosmosis are coupled processes
114
Q

ATP Synthase

A

Allows H⁺ protons to cross the membrane into the mitochondrial matrix

115
Q

Chemiosmosis

A

Diffusion of H⁺ protons across the membrane down the gradient, resulting in ATP

116
Q

Enzymes in ETC

A
  1. NADH reductase
  2. Succinate Dehydrogenase
  3. Cytochrome reductase
117
Q

Fermentation

A
  • Alcohol fermentation (produces alcohol)
  • Lactic acid fermentation (produces lactic acid)
  • Anaerobic
  • Only produces x2 ATP
118
Q

Amylase

A

Breaks down starch