Bio Test 2 Flashcards
Prokaryote
Unicellular organisms have this type of cell. Some features include:
- Not having a nucleus
- Lacks organelles, organization
- Smaller than eukaryotes
Eukaryote
Multicellular organisms have this type of cell. Some features include:
- Having a nucleus
- Having organelles
- Larger than prokaryotes
Cilium (Cilia)
- Present in eukaryotes only
- Functions to either move the cell itself or move things outside the cell (in the environment)
- Tiny hair-like threads on the surface of the cell (but still considered membrane-bound)
Flagellum (Flagella)
- Present in both prokaryotes and eukaryotes (sperm cell is the only flagellum in the human body)
- Motile
- Thread-like tail structure
Osmotic Bursting / Cytolysis / Osmotic Lysis
When the cell bursts due to an osmotic imbalance. The cell gains too much water through diffusion and ends up rupturing. Cell wall prevents this phenomenon.
Cell Wall
- Found in some prokaryotes and some eukaryotes (plants)
- Mainly made of cellulose
- Covers the cell membrane
- Provides protection; tough surface
- Prevents osmotic bursting
- Fully permeable
Pilus (Pili)
- Found only in prokaryotes
- Functions to help cells with adhesion or attachment to other cells
- Thin and hair-like structure, smaller than a flagellum
Nucleoid
Region in a prokaryote where DNA is found
Glycocalix / Capsule / Slime Layer
- Sticky outer layer of a prokaryote
- Glycocalix means “sugar coat”
- Made of polysaccharides and proteins
- Helps bacteria evade immune system more easily
Biofilm
Groups of bacteria sticking together, creating slimy coatings to protect them
3 Domains of Life are…
- Archaea (prokaryotic)
- Bacteria (prokaryotic)
- Eukarya (eukaryotic)
Gram-positive
Thick cell wall:
- Thick peptidoglycan layer
- No outer membrane
- Retains color in gram staining
Gram-negative
Thin cell wall:
- Thin peptidoglycan layer
- Has an outer membrane (lipopolysaccharides)
- Does not retain color in gram staining
Gram Staining
Involves crystal violet dye, iodine solution, and ethyl alcohol or acetone.
- Crystal violet dye + iodine solution form crystal violet-iodine complex
- Ethyl alcohol or acetone dehydrates peptidoglycan layer, causing it to shrink
- In gram-positive bacteria, the violet-idoine complex cannot penetrate the shrunken peptidoglycan layer, causing it to have a purple color
- In gram-negative bacteria, the peptidoglycan layer is too thin to hold the complex crystals, causing color to be lost
Differential Staining
A technique where you use different dyes to stain different microorganisms on a slide, allowing you to differentiate between the two of them more easily
Lipopolysaccharide (LPs)
- Part of the outer membrane in gram-negative bacteria (and the outer membrane is part of the cell wall)
- Consists of a lipid and polysaccharide
- Endotoxin, harms the human body
Peptidoglycan
- Part of the cell wall in bacteria
- Thicker layers in gram-positive bacteria
- Thinner layers in gram-negative bacteria
- Made of, as the name implies, glycan (anything with glycosidic linkages) chains and short peptides
Nucleus
- Where DNA is stored
- Contains the nucleolus
- Contains chromatin
- Has a nuclear membrane made of two layers
- Has nucleus pores
Nuclear DNA
- Term used to describe DNA found in the nucleus of a cell
- Both paternal and maternal DNA
Histones
Proteins in chromatin that help give structure to chromosomes (made of DNA)
Chromatin
Histones + DNA
Nucleolus
- Transcribes/Produces rRNA
- Produces ribosomes
Nuclear Membrane / Nuclear Envelope
- Made of two phospholipid bilayers (inner and outer)
- Semi-permeable
Nucleus Pores
- Holes in the nuclear membrane
- Allows select molecules to pass (such as RNA)
Rough Endoplasmic Reticulum
- Site of protein synthesis (produces proteins)
- Contains ribosomes
- Makes membrane
Ribosomes
- Functions to actually synthesize and produce proteins
- Found in the rough ER
- Made in the nucleolus
- Has a large subunit and small subunit
- Made of rRNA and protein
Smooth Endoplasmic Reticulum
- Synthesizes lipids
- Metabolizes carbohydrates
- Detoxification of drugs and poisons
- Makes vesicles (membranes of phospholipid bilayer + proteins)
Golgi Apparatus
- Modifies lipids and proteins
- Sends them to destinations in vesicles
- Produces vesicles just like the smooth ER does
DNA Cycle
DNA → mRNA → out of nuclear pores → rough ER → ribosome → protein → smooth ER → vesicle → golgi apparatus → vesicle → cell membrane
Mitochondrion (Mitochondria)
- Found in eukaryotic cells
- Produces ATP via cellular respiration
- Has its own ribosomes (can make proteins)
- Has its own DNA
- Mitochondrial DNA is maternal only
Lysosome
- ONLY for animal cells, NOT in plant cells
- Has hydrolytic enzymes (enzymes involved in hydrolysis)
- Involved in breaking down worn-out or excess cell parts
- Can also destroy viruses or bacteria
- “Clean up” organelle
Central Vacuole
- ONLY for plant cells and fungi cells, NOT in animal cells
- Stores chemicals for later use
- Stores water to provide rigidity
- Stores macromolecules
- Storage organelle in plants
Chloroplast
- Only in plants
- Converts CO2 and H2O into sugar using light energy
- Has its own ribosomes
- Has its own DNA
- Made of thylakoids (in grana) and stroma
- Contains chlorophyll in grana
Thylakoid
- Flattened sacs in chloroplasts
- Photosynthesis takes place here
- Stacks of thylakoids are called grana (singular: granum)
Granum (Grana)
Stacks of thylakoids
Stroma
- Colorless fluid around the grana in chloroplasts
- Photosynthesis is started here and finished in thylakoids
Centrosome
- Only found in animal cells
- Gives structure to cell
- Primary microtubule-organizing center (MTOC), moves microtubules around, aka shifts cytoskeleton
- “Funny” vacuole
- Contains centrioles
Centriole/Centrosome
- Produces spindle fibers used to move chromosomes around the cell during mitosis (centriole)
- Involved in flagella and cilia formation (centrosome)
- Helps determine locations of organelles in the cell (centrosome)
Cytoskeleton
- Gives cell its structure
- Consists of 3 components: microtubules, microfilaments, and intermediary filaments
Endomembrane System
- How membrane-bound organelles interact
Fluid Mosaic
The idea that the plasma membrane is made of a mix of lipids, proteins, and carbohydrates
Plasma Membrane
- Aka cell membrane
- Phospholipid bilayer
- Selectively permeable, allows only specific molecules to pass
Membrane Fluidity
- Viscous: saturated hydrocarbon tails, straight
- Fluid: unsaturated hydrocarbon tails, kinked
- Cholesterol can fill in, making saturated tails more fluid and unsaturated tails more viscous (good balance)
Glycoprotein
- Protein attached to oligosaccharide
- Modified protein
- Involved in cell-to-cell recognition, virus-to-cell recognition, bacteria-to-cell recognition
- On the outside of a plasma membrane with the oligosaccharde facing out
Glycolipid
- Lipid attached to oligosaccharide
- Modified lipid
- Involved in cell-to-cell recognition, virus-to-cell recognition, bacteria-to-cell recognition
- On the outside of a plasma membrane with the oligosaccharde facing out
Phospholipid Movement
- Flips laterally (horizontally) about 10 million times per second
- Flip-flops or switches vertically about once per month
Simple Diffusion
- Passive transport
- Involves either hydrophobic molecules or small, uncharged, and polar molecules