bio 2 lab test 2

Question 1

nervous tissue is made of

Answer 1

neurons and Bilal cells (neruoglia)

Question 2

primary brain vesicles

Answer 2

forebrain
midbrain
hindbrain

Question 3

forebrain

Answer 3

cerebrum (mostly cortex + white matter)

Diencephalon (calamus + hypothalamus)

Question 4

midbrain

Answer 4

brain stem (pons)
cerebellum
brain stem (medulla oblongata

Question 5

gyri

Answer 5

elevated ridges

major anatomical landmarks

Question 6

sulci

Answer 6

grooves seperating gyro

landmarks

Question 7

fisures

Answer 7

deep sulcus

landmarcs

Question 8

longitudinal fissure

Answer 8

deep fissure separating right and left cerebral hemispheres

Question 9

central sulcus

Answer 9

divide from and parietal lon

Question 10

lateral sulcus

Answer 10

divides temporal love and parietal lobe

Question 11

frontal lobe

Answer 11

anterior portion of cerebral hemisphere
(controls muscles movements, speech (on left, Broca)) personality, intellect, complex learning, reasoning, abstract idea making)

Question 12

parietal lobe

Answer 12

between from and occipital lobe

• Somatic sensation
• General interpretation areas
• Language (left side; Wernicke’s area)

Question 13

temporal lobe

Answer 13

lateral sides of cerebral cortex

• General interpretation areas
• Hearing

Question 14

occipital lobe

Answer 14

back of cerebral hemisphere (vision)

Question 15

Primary somatosensory cortex

Answer 15

In postcentral gyrus of parietal lobe Receives impulses from body’s sensory receptors (eg, pressure, pain, temperature)`

Question 16

primary motor area

Answer 16

In precentral gyrus of frontal lobe Conscious or voluntary movement of skeletal muscles`

Question 17

somatosensory association area

Answer 17

Immediately posterior to primary somatosensory area Interpretation of sensory impulses

Question 18

wernickes area

Answer 18

Left hemisphere at the junction between parietal and temporal lobes Language area: interpretation of speech by recognizing spoken words

Question 19

brocas area

Answer 19

Left side of frontal lobe in most people Motor speech area

Question 20

auditory areas

Answer 20

Temporal lobes at the junction with the parietal lobes Auditory sensory and interpretation areas

Question 21

vision areas

Answer 21

Occipital lobe Visual sensory and interpretation areas

Question 22

brain stem

Answer 22

a) midbrain: relay motor output from cerebrum to pons, medulla oblongata, spinal cord. relates sensory input from spinal cord to thalamus
coordinated movements of eyes, head, neck to auditory and visual stimuli
b)pons:
- Relays information from different brain areas to the cerebellum
- Together with the medulla oblongata, helps control breathing
D)medulla oblongata:
- Relays motor and sensory information between spinal cord and different brain areas
- Controls of consciousness and arousal
- Regulates breathing, cardiac functions, and blood vessel diameters

Question 23

outer vs deeper area

Answer 23

outer: grey
inner: white.

Question 24

what do sheep have smaller

Answer 24

fronttal lobe

Question 25

olfactory bulb sheep vs human

Answer 25

sheep is bigger

better smell

Question 26

mamillary body in human vs sheep

Answer 26

bigger in sheep

Question 27

Bacteria lab protocol

Answer 27

a) 250 microL of transformation solution into 2 microcentrofuge tubes (creates channels in cell wall and plasma membrane of bacteria so DNA can go in) they are competent cells when treated
2) put on ice and put 2-4 big coli colonies into tubes using a sterile loop and mix
3) add 0.8 x 10-1 μg/μL pGLO plasmid into one tube (using loop/10micro litter)
4) ice for 10 mins
5) put into 42 degree water bath for 50 sec then on ice for 2 mins
6) add 25μL of LB to both tubes then put at room temp 10 mins
7) put 100μL of solution on each labeled plate (+/- LB amp and +/-LB amp ara) spread using loops
8) stack and tape together upside down in 37 degrees for the night

Question 28

bacterial transformation

Answer 28

adding a gene into an organism to change the trait

Question 29

Bacterial transformation efficiency equation

Answer 29

nbr of colonies on +lb/amp/ara under uv light/

amount of dna on +lb/amp/ara plate

Question 30

amount of dna on +lb/amp/ara plate calculation

Answer 30

=volume of DNA put on/total sample volume

=100(PGLO) / 510 (PGLO+LB+transformation solu)

Question 31

IGg
IGa
IGm

Answer 31

iGA+m are right whille you have covid

IGg after having covid

Question 32

pcr protocol

Answer 32

1) swab cheek, put in saline, put some in micro tube
2) centrifuge 2 mins
3) take out supernant and resuspend pellet
4) put pellet into screw cap with 200microL of instagene
5) heat for 5 mins shake 5 mins at 56
6) shake then put in 100 for 5 mins
7) put some supernant into pcr tube with some of the master mix (yellow)
8) put in thermal cycler for 4 rounds, 3hrs, and then leave at -20
9) run electrophoresis

Question 33

without vs with ALU

Answer 33

with ought: 641

with: 941