BB LRGPHOR Ch4 - Euthanasia & Necropsy

Question 1

1. Pain is not experienced if what brain structures are non-functional?

Answer 1

Cerebral cortex and Subcortical structures

Question 2

7. True or False: A physical method must be followed after CO2 euthanasia to assure irreversibility.

Answer 2

True

Question 3

8. True or False: CO2 gas euthanasia may affect the yield of healthy embryo in transgenic mouse colonies.

Answer 3

FALSE

Question 4

9. What is the recommended percentage of the CO2 displacement in the euthanasia chamber?

Answer 4

20%

Question 5

10. True or False: CO2 gas may cause perivascular edema and extravasation of blood

Answer 5

True

Question 6

12. What is the correct order of inhalation anesthetics with the longest induction time?
    a. Isoflurane, halothane, enflurane, sevoflurane
    b. Halothane, enflurane, Isoflurane, sevoflurane
    c. enflurane, sevoflurane, Isoflurane, halothane
    d. halothane, Isoflurane, enflurane, sevoflurane

Answer 6

b

Question 7

13. True or False: Use of nitrogen and argon gases is conditionally acceptable for euthanasia in rabbits and rats.

Answer 7

True

Question 8

18. What is the cut off for using cervical dislocation for euthanasia in rats?

Answer 8

<200g

Question 9

20. True or False: Microwave irradiation can be used for euthanasia using appliances that are available for general public use.

Answer 9

F Specialized equipment is needed

Question 10

21. What is the cut off for using cervical dislocation method for rabbit euthanasia?

Answer 10

<1 kg

Question 11

22. True or False: Guinea pig fetuses that are 35 days may be euthanized by euthanasia of the dam.

Answer 11

FALSE

Question 12

23. True or False: Fetal hamsters less than 15 days old maybe euthanized by euthanasia of the dam.

Answer 12

True

Question 13

24. True or False: Rat and mouse fetuses that are less than 15 days into gestation, euthanasia of the dam is adequate for the fetuses.

Answer 13

True

Question 14

25. Mouse and rat neonates can be anesthetized using hypothermia up to what age?

Answer 14

Less than 6 days old

Question 15

32. Which organ cannot be visualized after skin removal?

Answer 15

Thymus gland

Question 16

34. True or False: In order to preserve the anatomical orientation of reproductive organs in mice during fixation, it is advisable to attach entire organs to a dry glass slide or an index card.

Answer 16

True

Question 17

35. True or False: In rats, the patency of the common bile duct should be assessed by opening proximal duodenum and applying pressure on the gallbladder.

Answer 17

Rats don’t have a gallbladder!

Question 18

43. Frozen tissue samples are suitable with which of the follow studies?
    a. Immunohistochemical
    b. Histochemical
    c. Immunofluorescence
    d. All of the above

Answer 18

d

Question 19

44. In most studies how soon the samples need to be fixed in formalin?

Answer 19

Within 30 minutes

Question 20

45. What is the rate of fixative (mm/hr) penetration through the tissue?

Answer 20

1 mm/hour

Question 21

46. The frozen tissue samples should be preserved in what temperature?

Answer 21

-80°C

Question 22

47. How many hours most tissue samples should be fixed in formalin?

Answer 22

24-48 hours

Question 23

48. What should be the volume of the fixative in the container?

Answer 23

10-20 times more than the tissue volume

Question 24

49. The ideal thickness of tissue samples is:

Answer 24

5 mm

Question 25

50. True or False: A major disadvantage of formalin fixation is the continuous formation of cross-linking amino groups resulting in conformation changes.

Answer 25

True