BB LRGPHOR Ch4 - Euthanasia & Necropsy Flashcards
1
Q
- Pain is not experienced if what brain structures are non-functional?
A
Cerebral cortex and Subcortical structures
2
Q
- True or False: A physical method must be followed after CO2 euthanasia to assure irreversibility.
A
True
3
Q
- True or False: CO2 gas euthanasia may affect the yield of healthy embryo in transgenic mouse colonies.
A
FALSE
4
Q
- What is the recommended percentage of the CO2 displacement in the euthanasia chamber?
A
20%
5
Q
- True or False: CO2 gas may cause perivascular edema and extravasation of blood
A
True
6
Q
- What is the correct order of inhalation anesthetics with the longest induction time?
a. Isoflurane, halothane, enflurane, sevoflurane
b. Halothane, enflurane, Isoflurane, sevoflurane
c. enflurane, sevoflurane, Isoflurane, halothane
d. halothane, Isoflurane, enflurane, sevoflurane
A
b
7
Q
- True or False: Use of nitrogen and argon gases is conditionally acceptable for euthanasia in rabbits and rats.
A
True
8
Q
- What is the cut off for using cervical dislocation for euthanasia in rats?
A
<200g
9
Q
- True or False: Microwave irradiation can be used for euthanasia using appliances that are available for general public use.
A
F Specialized equipment is needed
10
Q
- What is the cut off for using cervical dislocation method for rabbit euthanasia?
A
<1 kg
11
Q
- True or False: Guinea pig fetuses that are 35 days may be euthanized by euthanasia of the dam.
A
FALSE
12
Q
- True or False: Fetal hamsters less than 15 days old maybe euthanized by euthanasia of the dam.
A
True
13
Q
- True or False: Rat and mouse fetuses that are less than 15 days into gestation, euthanasia of the dam is adequate for the fetuses.
A
True
14
Q
- Mouse and rat neonates can be anesthetized using hypothermia up to what age?
A
Less than 6 days old
15
Q
- Which organ cannot be visualized after skin removal?
A
Thymus gland
16
Q
- True or False: In order to preserve the anatomical orientation of reproductive organs in mice during fixation, it is advisable to attach entire organs to a dry glass slide or an index card.
A
True
17
Q
- True or False: In rats, the patency of the common bile duct should be assessed by opening proximal duodenum and applying pressure on the gallbladder.
A
Rats don’t have a gallbladder!
18
Q
- Frozen tissue samples are suitable with which of the follow studies?
a. Immunohistochemical
b. Histochemical
c. Immunofluorescence
d. All of the above
A
d
19
Q
- In most studies how soon the samples need to be fixed in formalin?
A
Within 30 minutes
20
Q
- What is the rate of fixative (mm/hr) penetration through the tissue?
A
1 mm/hour
21
Q
- The frozen tissue samples should be preserved in what temperature?
A
-80°C
22
Q
- How many hours most tissue samples should be fixed in formalin?
A
24-48 hours
23
Q
- What should be the volume of the fixative in the container?
A
10-20 times more than the tissue volume
24
Q
- The ideal thickness of tissue samples is:
A
5 mm
25
Q
- True or False: A major disadvantage of formalin fixation is the continuous formation of cross-linking amino groups resulting in conformation changes.
A
True
