Basic Techniques of Mol Bio

Question 1

Why are biotechniques important?

Answer 1

Diagnosis
Treatment
Familial inheritance
Forensics

Question 2

What is the purpose of PCR?

Answer 2

Amplify specific DNA sequence

Question 3

What are the requirements for PCR?

Answer 3

DNA primers
Heat stable DNA pol
Thermocyclers

Question 4

What is recombinant DNA technology?

Answer 4

To clone and manipulate genes for study or applications

Question 5

What is restriction endonuclease used for?

Answer 5

Cutting DNA into manageable and precise fragments
Recognize specific sequences - palindromes
Overhanging ends allows for cloning

Question 6

What are vectors?

Answer 6

Vehicle for carrying DNA
Able to expand number of copies
Can be used to produce proteins

Question 7

What are the two DNA libraries?

Answer 7

Genomic - complete
Complementary - expressed

Question 8

What is southern blotting?

Answer 8

Analysis of DNA
Used to detect mutations and polymorphisms

Question 9

What is northern blotting?

Answer 9

Analysis of RNA - specific RNA sequences
Determine lengths of RNA

Question 10

What is western blotting?

Answer 10

Analysis of proteins