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Bacteriology > Basic Bacteriology Facts > Flashcards

Basic Bacteriology Facts Flashcards

1
Q

What is the outer membrane of gram negatives composed of?

A

Lipopolysaccharide (LPS)

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2
Q

What structure of gram-negatives contains the beta-lactamases?

A

Periplasm - space between the cytoplasmic membrane and outer membrane

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3
Q

What are biofilms composed of?

A

Glycocalyx - a loose network of polysaccharides

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4
Q

What bacteria do not Gram stain well and why (give reason for each bug)?

A

Mneumonic - These Microbes May Lack Real Color

Treponema - too thin to be visualized
Mycobacteria - high lipid content
Mycoplasma - no cell wall
Legionella pneumophila - primarily intracellular
Rickettsia - intracellular parasite
Chlamydia - intracellular parasite
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5
Q

What bugs need Giemsa stain?

A

Mneumonic - Certain Bugs Really Try my Patience

Chlamydia
Borrelia
Rickettsia
Trypanosomes
Plasmodium
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6
Q

What bugs are stained with Ziehl-Neelsen (carbol fuchsin) stain?

A

Acid fast bacteria (Nocardia, Mycobacteria)

Protozoa (cryptosporidium oocysts)

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7
Q

What bugs are stained with India ink?

A

Cryptococcus neoformans

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8
Q

What bugs are stained with silver stain?

A

Fungi
Legionella
H. pylori

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9
Q

What special culture/media is required for isolation of H. influenzae?

A

Chocolate agar

Contains Factors V and X

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10
Q

What special culture/media is required for isolation of Neisseria gonorrhoeae and Neisseria meningitidis?

A

Thayer-Martin agar

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11
Q

What special culture/media is required for isolation of Bordetella pertussis?

A

Bordet-Genou agar

Regan-Lowe medium

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12
Q

What special culture/media is required for isolation of C. diptheriae?

A

Tellurite agar

Loffler medium

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13
Q

What special culture/media is required for isolation of Mycobacterium tuberculosis?

A

Lowenstein-Jensen agar

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14
Q

What special culture/media is required for isolation of Mycoplasma pneumoniae?

A

Eaton agar

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15
Q

What special culture/media is required for isolation of Lactose-fermenting enterics?

A

MacConkey agar

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16
Q

What special culture/media is required for isolation of E. coli?

A

Eosin-methylene blue (EMB) agar

17
Q

What special culture/media is required for isolation of Legionella?

A

Charcoal yeast extract agar buffered with cysteine and iron

18
Q

What special culture/media is required for isolation of Fungi?

A

Sabouraud agar

“Sab’s a fun guy!”

19
Q

What are obligate intracellular bugs?

A

Rickettsia
Chlamydia
Coxiella

20
Q

What are facultative intracellular bugs?

A

Mneumonic: Some Nasty Bugs May Live FacultativeLY

Salmonella
Neisseria
Brucella
Mycobacterium
Listeria
Francisella
Legionella
Yersinia pestis

Bacteriology (19 decks)

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