Bartling Pt. 2 Flashcards
intrinsic enzymatic activity receptors are ligand ____
ligand triggered protein kinases
intrinsic receptors form dimers upon
dimers upon ligand bonding
phosphorylation activates
other protein kinases
ion channel receptors change confirmation from
ligand binding
ion channel receptors allow specific ions to
flow through channel
botulinum toxin affects ion channels directly/non directly
not directly
botulinum toxin prevents release of
acethylcholine neurotransmitter
botulinum toxin cleaves proteins involved in
docking of neurotransmitter vesicles
intracellular/extracellular calcium concentration gradient
100nM/1mM
calcium gradient enables rapid changes in Ca concentration via
hormone ligation
calcium binds to
calmodulin (induces conformational change)
calmodulin: two globular domains joined by a
long alpha helix
calcium/calmodulin complex binds to and modifies
target kinase proteins that initiate signal cascade
PIP2 is second messenger responsible for
responsible for calcium mobilization
PIP2 is hydrolized by
phospholipase C (PLC)
hydrolized PIP2 generates what 2 second messengers
IP3 and DAG
IP3 is ____ soluble and mobilizes _____
water soluble and mobilizes calcium
DAG is anchored in ____ and activates key protein ____
anchored in plasma membrane (hydrophobic fatty acid side chains) and activates protien kinase C (PKC) family
DAG generated by
PLC
phosphatidic acid generated by
PLD
arachidonic acid generated by
PLA2
arachidonic acid and prostaglandins are key
inflammatory and pain mediator
arachidonic acid is precursor of
eicosanoids
eicosanoids examples
prostaglandins, luekotrienes
eicosanoids act like
hormones and signal via G-protein coupled receptors
arachidonic acid conversion to prostaglandins involves a
cyclooxygenase isoforms
