Bacterial/virus growth and identification

Question 1

How do bacteria multiply?

Answer 1

Binary fission

Question 2

What is the name for doubling time in bacterial growth?

Answer 2

Generation time

Question 3

Oxygen requirements - NEED oxygen

Answer 3

obligate aerobes

Question 4

NO oxygen (can be damaged with O2)

Answer 4

obligate anaerobes

Question 5

Can grow with or without O2

Answer 5

Facultative anaerobes

Question 6

Require small amount of O2 to grow

Answer 6

Microaerophiles

Question 7

Cannot use O2, but can tolerate its presence

Answer 7

Aerotolerant anaerobe

Question 8

What can a microscope measure for bacterial growth?

Answer 8

Direct cell counts

Question 9

Counting colony forming units

Answer 9

Viable cell counts

Question 10

Turbidity (cloudiness) used to measure…

Answer 10

Biomass

Question 11

True or false - viruses cannot grow on petri dishes

Answer 11

True

Question 12

Clearings where bacteriophage killed bacteria

Answer 12

Plaques

Question 13

Media that prevents the growth of certain microbes

Answer 13

Selective

Question 14

Makes certain microbes grow with a visual difference from others

Answer 14

Differential

Question 15

General media that allows for the growth of most microbes

Answer 15

Enriched

Question 16

Media rich in nutrients that contain water soluble extracts of plant or animal tissue

Answer 16

Complex

Question 17

Test to amplify the 16s rRNA region of DNA sequencing

Answer 17

PCR testing

Question 18

Enzymes used as bacterial immune system against viruses

Answer 18

Restriction enzymes

Question 19

Name to describe optimal temperature of common bacteria like E. coli?

Answer 19

Mesophiles (25 - 45 degrees C)

Question 20

Order of names for optimum growth temperatures

Answer 20

Psychrophiles, psychrotrophs, mesophiles, thermophiles, hyperthermophiles (usually archaea)

Question 21

Microbes that grow best between pH of 5 and 8

Answer 21

Neutrophiles

Question 22

Obtain energy from sunlight

Answer 22

Phototrophs

Question 23

Obtain energy from chemical compounds

Answer 23

Chemotrophs