Bacterial/virus growth and identification Flashcards
How do bacteria multiply?
Binary fission
What is the name for doubling time in bacterial growth?
Generation time
Oxygen requirements - NEED oxygen
obligate aerobes
NO oxygen (can be damaged with O2)
obligate anaerobes
Can grow with or without O2
Facultative anaerobes
Require small amount of O2 to grow
Microaerophiles
Cannot use O2, but can tolerate its presence
Aerotolerant anaerobe
What can a microscope measure for bacterial growth?
Direct cell counts
Counting colony forming units
Viable cell counts
Turbidity (cloudiness) used to measure…
Biomass
True or false - viruses cannot grow on petri dishes
True
Clearings where bacteriophage killed bacteria
Plaques
Media that prevents the growth of certain microbes
Selective
Makes certain microbes grow with a visual difference from others
Differential
General media that allows for the growth of most microbes
Enriched
Media rich in nutrients that contain water soluble extracts of plant or animal tissue
Complex
Test to amplify the 16s rRNA region of DNA sequencing
PCR testing
Enzymes used as bacterial immune system against viruses
Restriction enzymes
Name to describe optimal temperature of common bacteria like E. coli?
Mesophiles (25 - 45 degrees C)
Order of names for optimum growth temperatures
Psychrophiles, psychrotrophs, mesophiles, thermophiles, hyperthermophiles (usually archaea)
Microbes that grow best between pH of 5 and 8
Neutrophiles
Obtain energy from sunlight
Phototrophs
Obtain energy from chemical compounds
Chemotrophs
