Bacterial Morphology And Growth Flashcards

1
Q

What are methods of classifying bacteria?

A
Size 
Gross structure 
Cell wall structure 
Differential stains 
Morphology 
Growth requirements
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2
Q

What are structural components of bacteria?

A
All bacteria contain DNA 
Haploid DNA 
DNA in nucleoid region => not bound to nucleus 
Ribosome in cytoplasm => different to eukaryotic cells 
Peptidoglycan membrane 
No mitochondria 
No membrane bound organelles 
\+/- capsule 
\+/- pilli/flagella
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3
Q

Describe the cell wall of gram positive bacteria?

A

Thick peptidoglycan layer with lipoteichoic acid

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4
Q

Give an example of gram positive bacteria

A

Streptococcus pyogenes

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5
Q

Describe the cell wall of gram negative bacteria?

A

Extra outer membrane with integral lipopolysaccharide

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6
Q

Give an example of gram negative bacteria?

A

Escherichia Coli

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7
Q

Describe the cell wall of acid fast bacteria?

A

Thick outer layer of mycolic acids linked by Arabina- galactas

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8
Q

What is gram stain?

A

A differential cell wall stain dividing bacteria

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9
Q

What colour indicates gram positive bacteria?

A

Blue/purple

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10
Q

What colour indicates gram negative bacteria?

A

Red/pink

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11
Q

In acid fast stain, what colour does acid fast bacteria go?

A

Red

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12
Q

What are factors affecting growth?

A
Division rate 
Lag phase time 
O2 availability 
Carbon availability 
Temp
pH
Inhibitors and growth factors
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13
Q

Growth rate

Describe the different groups based on growth rate

A

Rapid growers => division every 20-40 mins
Slow growers => division every 30hrs
Dormancy => 18hrs - 80years

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14
Q

What are the different groups of bacteria based on O2 requirements?

A

Obligate aerobes
Facultative anaerobes
Aerotolerant anaerobes
Obligate anaerobes

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15
Q

What are different groups based on temp requirements for growth?

A

Pyschrophiles
Mesophiles
Thermophiles
Extreme (hyper) thermophiles

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16
Q

What are different groups of bacteria based on pH requirements?

A

Acidophiles
Neutrophiles
Alkaphiles