Bacteria in the Genitourinary Tract Flashcards
E. coli will test positive for which of the following tests?
A. Indole
B. Citrate
C. VP
D. Methyl Red
A. Indole
D. Methyl Red
What is the most severe UTI?
pyelonephritis or urosepsis
58% of UPEC belongs to which serotypes?
O1, O2, O4, O6, O8, O9, O18, O83
Of the 10 pathogen-specific genome islands, 3 contain ____
P fimbriae and other virulence factors
Why are women more susceptible to UPEC?
proximity of urethra
Enumerate all 7 UPEC Components
- LPS
- Capsular (K protein)
- toxins
- iron acquisition systems
- flagellar (H) Ag (O:K:H serotype)
- fimbrial and afimbrial adhesins
- haemolysin
TRUE OT FALSE: UPEC lacks the type III secretion
system to inject VFs
TRUE
What is the most external part of cell wall for UPEC?
O Ag
This structure is made up of repeating units of polysaccharide that makes UPEC resistant to heat and alcohol.
O Ag
The capsule is external to the ____ and serves as the attachment to ______.
lipopolysaccharide, epithelial cells
What UPEC structure may interfere with agglutination by anti-O Ab?
Flagellar (H ag)
The flagella may be denatured or removed by ______
heat or alcohol
Enumerate fimbriae types of UPEC
- Type 1
- P fimbriae
- F1C
- S Fimbriae
- Ygi and Yad
FimA: Main structural subunit; FimH: ____
Adhesin, at the tip
What is the function of Type 1 fimbriae?
invade bladder cells by binding mannose moietis and increase inflammation
The presence of this pili is a marker of a highly virulent strain
pap: pyelonephritis-associated pili
What is the function of P fimbriae?
enhance severity of UTI by promoting adherence to vascular endothelium & bladder epith
P fimbriae is mannose-_______, and is specific to _ blood group antigen
mannose-resistant, specific to O blood
TRUE OR FALSE: papG is the main subunit of P fimbriae
FALSE, papA
What is the function of F1C fimbriae?
adherence to the endothelium and muscular layer of the bladder kidneys: distal tubules and collecting ducts
Which fimbriae type is genetically identical to F1C except for the tip adhesin?
S fimbriae
SfaS (tip adhesin) binds _______ on kidney epithelial cells
sialic acid
What is the function of S fimbriae?
binds to kidney tubules, collecting ducts, glomerulus, and epith and musc. layers of bladder
What is the function of Ygi & Yad fimbriae?
biofilm formation
1: Ygi - kidney epith cells
2: Yad: Muscular epith cells
A. First statement is correct. Second is Wrong
B. First statement is wrong. Second is correct.
C. Both statements are true
D. Both statements are false
A. Yad is for bladder epith. cells
______ are proteins that undergo type V secretion
Autotransporters
Autotransporters are secreted into ______ or remain attached to bacterial cell surface.
extracellular matrix
TRUE OR FALSE; Autotransporters is involved in neuropathogenesis and promotes biofilm formation.
FALSE: uropathogenesis
UPEC pathogenesis is ____ and _____
ascending and hematogenous
Signs and Symptoms of UPEC
- Urinary frequency
- Dysuria
- hematuria
- pyuria
- flank pain
Dysuria is characterized by ____
Sensation of pain and/or burning, stinging, or itching of the urethra or urethral meatus associated with urination (NCBI)
Which of the the following are NOT treatment drugs for UPEC?
sulfonamides
ampicillin
penicillin
cephalosporins
fluoroquinolones
aminoglycosides
penicillin
Individually, Neisseriae appears _____-shaped
Kidney
In pairs, Neisseriae the _____ sides are adjacent
flat or concave
Neisseriae appears ______, ______, ______, ______ in enriched media
convex, glistening, elevated, mucoid colonies
TRUE OR FALSE: Neisseriae appears pigmented, hemolytic in media
FALSE: transparent/opaque,
nonpigmented, nonhemolytic
Neisseriae grows best under _______ conditions
aerobic
What test is key in identifying Neisseriae species?
Oxidase Test
Which bacteria is nonmotile, non-spore-forming, non-acid-fast?
Neisseriae
Neisseria produces ______ enzymes which causes rapid swelling and lysis at 25 C, alk pH
autolytic
Neisseriae grows best in __% CO2
5%
Neisseriae is rapidly killed by:
drying
sunlight
moist heat
disinfectants
TRUE OR FALSE: Neisseria has bigger colonies than neisseriae
False
N. gonorrhoeae requires ____, _____, and ___ for growth
arginine, hypoxanthine, uracil
N. gonorrhoeae can grow in which of the following carbohydrates
Maltose
Lactose
Sucrose
Glucose
Fructose
Glucose
Enumerate N. gonorrhoeae surface structures
- Pili (fimbriae)
- POR (fimbriae)
- OPA Proteins
- RMO (Protein III)
- LIPOOLIGOSACCHARIDE
- H8
- Fbp
- IgA1 Protease
This protein associates with Por in formation of pores
RMP (protein III)
This N. gonorrhoeae structure allows nutrient entry as wells as prevention of phagosome-lysosome fusion
POR
Which terminal of pili is most prominent in immune response?
Amino terminal
Mid portion
C Terminal
C terminal
What is the function of OPA proteins?
adhesion w/in colonies
attachment to host cells
LOS resemble what human structure?
cell membrane glycosphingolipids