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BFCP 1 FINAL > Bacteria Identification > Flashcards

Bacteria Identification Flashcards

1
Q

Supportive culture media

A

Allows growth of most organisms
Blood agar

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2
Q

Enrichment culture media

A

Nutrient specificity
Chocolate agar

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3
Q

Selective culture media

A

Supresses growth of unwanted bacteria
MacConkey agarD

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4
Q

Differential culture media

A

Metabolic characteristics

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5
Q

Wet mount

A

Put on glass slide

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6
Q

Gram staining

A
  1. smear on plate
  2. crystal violet
  3. iodine = set
  4. ethanol = decolonized, desolve outermost membrane (gram -)
  5. red saffron = counter stain
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7
Q

Acid fast staining

A

Acid fast positive - red
Acid fast negative - blue
High amounts of lipids

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8
Q

Microscopy

A

Darkfiled
Electron
Fluorescent

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9
Q

Colony morphology

A

Hemolysis
a - partial - greenish
b - complete - hole around colonies
gamma - none

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10
Q

Pigmentation

A

Change in color of plate
Pink MacConkey will switch to yellow with non-lactose fermenting bacteria, stay pink with lactose fermenters

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11
Q

Swarming

A

Motility

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12
Q

Catalase

A

Organism that produce catalase, important for breakdown of O2 byproducts

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13
Q

Carb utilization

A

Color change depending on pH

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14
Q

Coagulase

A

Clot blood

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15
Q

Oxidase

A

Presence of cytochrome oxidase enzyme - aerobic respiration

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16
Q

Indole

A

Degrade tryptophan to get indole

17
Q

Immunological

A

ELISA - test antibodies

18
Q

Automated systems

A

Mass spectrometry

19
Q

Antimicrobial susceptibility testing

A

Guide for reactions with antibodies

20
Q

MIC

A

Concentration that inhibits growth

21
Q

MBC

A

Concentration that kills bacteria

22
Q

Bacteriostatic

A

Inhibits growth
MBC > 4 X MIC

23
Q

Bactericidal

A

Kills
MBC = MIC

24
Q

Kirby Bower

A

Disk diffusion
Antibiotics put on film of bacteria, zone of inhibition = diameter of circle - determine if susceptible, resistant, intermediate

25
Q

Broth dilution

A

Determine MIC
Add bacteria to tube with diff [ ]
Cloudy = growth, clear = no growth
Can plate to determine MBC

26
Q

E-test

A

Determine MIC
Antibiotic on plate, diffusion gradient

BFCP 1 FINAL (13 decks)

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