Bacteria - Hillard Flashcards
bacteria have what for DNA
plasmids
circular DNA that can be transducted, transformed, or conjugated to other bacteria
Generalized Transduction
Specialized transduction
Generalized : virus of bacteria picks up any part of bacterial genome
Specialized : bacteria or virus is integrated into genome of bacteria and picks specific portion of genome during activation.
Gram + cell wall
thick with peptidoglycans+ Techoic and Lipoteichoic acids
Gram - cell wall
thin with high lipid washed away with alcohol + polysaccharides
**Lipid A ENDOTOXIN
Staining Gram - bacteria cells
- Crystal Violet - nothing
- Iodine - nothing
- Alcohol - removes color
- Safranin (counterstrain or coloring it pink)
Gram + staining
- Crystal Violet - crystal violet (purple)
- Iodine - dye tapping agent (purple)
- Alcohol - nothing
- Safranin - nothing
staining exception in what
- Gram+ Clostridium + Bacillus (mix purple and pink)
2. Acid Fast Bacilli (TB), uses acid decolorizer (red)
unique features of bacillus
some have spores and some are club shaped
coccobacillus shape
rod and sphere shaped
spirochetes
helical shaped
curvilinear
spirilla shaped
spiral or helical shaped stains used
- silver stains
- dark field microscopy
- AB immunohistochemical stains
shape of streptococcous pnemoniae
diplococci
macule
flat area of discoloration < 1cm
Patch
flat circumscribed area > 1cm
Papule
a circumscribed , elevated , solid lesion < 1cm
Plaque
circumscribed elevated solid lesion > 1cm
Nodule
a palpable solid lesion > 1cm
Vesicles
small, superficial, circumscribed blister < 1cm
Ulcer
an open sore or wound (break of skin or mucous membrane)
Cocci catalase +
Staphylococcus
Cocci Catalase -
Streptococcous
Enterococcus
Bacilli , Spore forming
Bacillus (grow in O2)
Clostridium (anaerobic)
Bacilli, Non-spore forming
Corynebacterium (club shape, non mobile)
Listeria (motile at 25 degree C)
Catalase + looks like
function
reduce H2O2 to bubbles
to defend itself against M and N
3 types to staph
- staphylococcus aureus
- Staphylococcus epidermis
- Staphylococcus saprophyticus
coagulase + in Staph
Staphylococcus aureus = activates fibrin clot
coagulase - in Staph
Staphylococcus epidermis
Staphylococcus saprophyticus
Staphylococcus Aureus
proteins
color
gram
Protein A, Penicillinase, Leukocidins, Hemolysins
Staphokinase (clot destroyer), Hemolysins
Golden
Gram +
Hemolysins
destroys RBSCs
Protein A
prevents AB mediated binding and complement activation
4 SX in Staphylococcus aureus
- Pneumonia : after previous viral infection
- Endocarditis
- Superficial skin and underlying soft tissue infections
- Septic Osteomyelitis in < 12yo + elderly
- Gasteroenteritis
Staphylococcus aureus pneomonia
high fever, necrotizing pneumonia, PRODUCTIVE COUGH, LUNG ABSCESSES
Staphylococcus aureus skin stuff
Folliculitis, Impetigo, Cellulitis, Abscesses
pealing of skin = TSS
Staphylococcus aureus osteomyelitis fluid
high N and cloudy
Staphylococcus aureus endocarditis
ACUTE , fever, chills, weakness, IV drug user= tricuspid
rare effect of Staphylococcus aureus
meningitis, brain abscess
Staphylococcus aureus Gasteroenteritis
food contamination ,
ACUTE N, V, D, ABD pain from preformed enterotoxin**
Staphylococcus aureus scaled skin syndrome
usually in children
from EXFOLIATIVE TOXIN A + B
fever, poor feeding
3 SX of toxic shock syndrome and what causes it
TSST-1 toxin
1. Gasteroenteritis
2. Rash on palms and soles (pealing)
3 .Shock, Hypotension, death
2 other then TSS diseases that show sole and palm rash with pealing
- Syphilis
2. rocky mountain spotted fever
what is toxic in tampons
superantigen releasing TNF and IL1
Staphylococcus epidermis virulence factor
Biofilms = extracellular polysaccharide matrix adhering to foreign device + barrier against immune system
Staphylococcus epidermis infects how
Nosocomial infection (epidermidis) = prosthetic components like heart valves, hardware, catheters, shunts **** endocarditis it gets into blood
Staphylococcus epidermis epidermidis
endocarditis, high fever, redness of skin
Staphylococcus saprophyticus causes what
UTI most common community acquired one
“honeymoon cystitis”
Streptococcus is classified by growth pattern
B-hemolysis (complete)
a- hemolysis (partial)
y- hemolysis (no)
B-hemolysis (complete)
complete breakdown of of blood = clear ring around circular colony
a- hemolysis (partial)
makes green ring around colony
Streptococcus is classified by Lancefield classification
*based on C carbohydrate group
= Group A and Group B
= Lancefield classification
Streptococcus Pyrogenes
classification
virulence factor
EX: most common
B-hemolytic + Group A
M-protein —–I complement
Streptococcal pharyngitis (exudate on tonsils, fever, lymphadenopathy)
what can streptococcus pyrogenes lead to if untreated
- rheumatic fever
2. post-strep glomerulonephritis
Streptococcus pyrogenes
Streptococcal pharyngitis sx
(exudate on tonsils, fever, lymphadenopathy)
Streptococcus pyrogenes
Scarlet fever SX
fever, red rough rash on trunk and neck = “sandpaper” NOT ON FACE, SOLE, PALM
red strawberry tongue
+ pharyngitis
scarlet fever caused by what and in what age
pyrogenic endotoxin
school children
Streptococcus pyrogenes
Erysipelas SX
sunburn looking rash on FACE
warm to touch
60-80yo
Streptococcus pyrogenes
Nectrotizing faciitis
ACUTE infection of fascia (CT)
= purple color + PAINFUL
= need surgery
Streptococcus pyrogenes
cellulitis and impetigo
Impetigo : golden crusty rash (children)
Cellulitis : infection on skin , swollen, warm, red, blotchy
Streptococcus pyrogenes
Rheumatic Fever SX
AB and T-cell reaction against M protein Ag
- fever HIGH
- migratory polyarthritis (joints)
- pancarditis (mitral valve regurg, pericarditis)
- Subcutaneous nodules (large bumps)
- Red ring shaped rash (erythema marginatum)
- Syndenham chorea (happing, halting gait, jerking, grimacing)
Rheumatic fever SX happen
1-5 weeks after initial acute infection (streptococcal pharyngitis)
Streptococcus pyrogenes
Post- Streptococcal Glomerulonephritis SX
AB-Ag complex deposited on glomerular BM
- edema
- HTN
- hematuria
- proteinuria
how to test for RF or Post-strep GN
detection of AB to streptolysin O and DNase B
Streptococcus agalactiae
classification
can cause
colonizes the
B- hemolytic + Group B
neonatal meningitis, pneumonia, sepsis
vagina
3 organisms that can cause neonatal meningitis
- streptococcus agalactiae
- Listeria monocytogenes
- E coli
Streptococcus pneumoniae classification shape colonizes virulence factor sensitive to
a-hemolytic diplococci with CAPSULE nasopharynx IgA protease * Optochin sensitive
Streptococcus pneumoniae
how to test for capsule
anti-capsular AB (QUELLING TEST) = swollen capsule
optochin
kills streptococcus pneumoniae
Streptococcus pneumoniae
pneumococcal pneumonia SX
(common community acquired pneumonia) 1. lobar consolidation in lungs 2. high fever, chills 3. cough, SOB, CP older then 65yo common
pneumococcal pneumonia after autopsy
CDC recommendation
heavy and diffuse consolidation
over 65yo get vaccine
Streptococcus pneumoniae
Meningitis
- high fever
- nuchal rigidity
- abnormal mental status changes
(you can also see headaches)
bacterial meningitis CSF
turbid looking
high N (normal is high monocytes)
low glucose
high protein
viral meningitis CSF
clear looking
high lymphocytes
some elevated protein
normal glucose
common cause of meningitis in children
Streptococccus pneuomoniae
common cause of meningitis in adolescence and young adulthood
Neisseria meningitides
Streptococcus pneumoniae
Otitis Media
middle ear infection (6mo-24mo most common)
- ear pain = pulling on ear
- hearing loss
- drainage
* ** Bulging of TM
3 organisma causing otitis media
- streptococcus pneumoniae (gram -)
- Haemophilus influenza (gram -)
- Moraxella catarrhalis (gram -)
spleen function
clears encapsulated bacteria
aslpenia / Hyposplenism caused by
- trauma
- sickle cell
- lymphoma
3 organisms that asplenic patients can easily get
- streptococcus pneumoniae
- Haemophilus influenza type B
- Neisseria meningitis
Streptococcus Viridans classification where is it colonized can cause sensitive to
a- hemolytic
Dental caries (cavities, S.mutans) + GI
subacute bacterial endocarditis
NOT sensitive to OPTOCHIN**
subacute endocarditis SX
in strep viridans
low fever , weakness, night sweats, low appetite, weight loss
common way to get Streptococcus viridans
dental cleaning thats invasive
organisms causing subacute bacterial endocarditis
- Streptococcus viridans
2. HACEK (also found in oral cavity flora)
Streptococcus Enterococci classification colonizes grow on what causes what kind of infection
y- hemolytic
normal bowel flora
bile + 6.5% NaCl
nosocomial opportunistic infections
Streptococcus Enterococci TX
resistant to most drugs like vancomycin
nosocomial opportunistic infections
wound infections, urinary tract, biliary tract, heart valves
Streptococcus Group D Non-Enterococci Classification colonizes grows on what causes what kind of infections
y-hemolytic
in GI also
Grow on ONLY BILE
nosocomial opportunistic infections (ESP S. Bovis = colon cancer)
what causes cavities
Streptococcus mutans
what causes colon cancer
Streptococcus Bovis
spore forming bacteria
Bacillus and Clostridium(anearobic)
2 O2 loving spore forming Bacilli organisms
- Bacillus cereus = Diarrhea
2. Bacillus anthracis = anthrax
4 Anaerobic spore forming Bacilli organism
- Clostridium difficile = nosocomial diarrhea
- Clostridium Botulinum = Botulism
- Clostridium tetani = tetanus
- Clostridium perfringes = gas gangrene
Bacillus cereus
resistant to
makes what
heat resistant even at cooking tempratures
makes enterotoxin over time even in hot food (when reheating food the toxin is there)
+ emetic toxin (not resistant to heat)
enterotoxin
ABD cramp, N,V within 1-3hrs
from reheating a dish
emetic toxin
not heat resistant
watery diarrhea within at least 8hrs
Bacillus anthracis
structure
resistant to
colonizes where
encapsulated, spore forming
drying and heat, chemicals
hides, herbivore animals, soil
Bacillus anthracis SX
- cutaneous anthrax : malignant pustule
- Inhalation anthrax : pulmonary spores —-> mediatinal lymph nodes = fever + myalgia –> mediastinal hemorrhage, sepsis, death
Inhalation anthrax can be seen as
X-ray : mediastinal widening due to hemorrhage
gastrointestinal anthrax and injectional anthrax
less common also from Bacillus anthrax
multiorgan failure and death
Clostridium Difficile
causes what
toxins made
what do you see in these
anaerobic spore forming bacilli
antibiotic -associated colitis (clindamycin, penicillin, cephalosporin)
1. Toxin A : inflammation, fluid secretion (D)
2. Toxin B : toxic to colonic epithelial cells (non-bloody D + ABD pain + fever)
pseudomembranous colitits
pseudomembranous colitits
many mucopurulent exudative plaques on GI
what causes nosocomial D
Clostridium Difficile —-> esp after AB use
Streptococcus Enterocolitis
other things causing pseudomembranous colitits
IBD
ischemic colitis
Clostridium Difficile can spread from
non washing hands , fecal to oral contamination
Clostridium Botulinum
classification
toxin effect
spore forming anaerobic bacilli
inhibits ACH release (neurotoxin)
Clostridium Botulinum spread in adults
food borne, undercooking, canned food, raw honey, smoked fish
Clostridium Botulinum SX in adults
Bilateral cranial neuropathy (vision changes, drooping of eyes and face) , descending muscle weakness, respiratory paralysis, death
Clostridium Botulinum spread in infants
honey, infant powder
Clostridium Botulinum SX in infants
CONSTIPATION —> due to difficulty swallowing, muscle weakness (hypotonia) = “floppy baby syndrome”
Clostridium Tetani
classification
colonizes
toxin effects
spore forming, anaerobic bacilli
soil, deep puncture wounds
Neurotoxin —-I GLYCINE and GABA = sustained motor contractions
Clostridium Tetani SX and prevention
- severe muscle spasm
- autonomic instability (tachy, sweating, labile BP (up and down)
- lockjaw
prevent by DTAP vaccine
Clostridium perfringens
classification
colonizes
toxin effects
spore forming, anaerobic bacilli
soil, deep wounds, trauma (can also from food, rare)
a- toxin (lecithinase) = lyse RBCs +endothelial cells = hemolysis and hemorrhage
Clostridium perfringens SX
- Cellulitis + wound infections = myonecrosis (muscle necrosis)
- pain edema, dark purple to black skin
- GAS GANGRENE , CO2 made by skin
- crepitus to palpation (gas in tissue)
Clostridium perfringes was seen alot in
and if eaten
WW1 and WW2 necrotizing enteritis (rare)
Corynebacterium diphtheriae classification shape toxin and effect prevention
non-spore, non-mobile bacilli
club shaped, pleomorphic
AB exotoxin : inactivates EF2 = no mRNA translation
vaccine DTAP
Corynebacterium diphtheriae grown on
Loefflers medium
black color
Corynebacterium diphtheriae SX
- fever, headache, cough, adenopathy,
- pharyngitis + pseudomembrane formation (yellow/gray exudate = DO NOT SCRAPE—-> systemic infection)
- Myocarditis (AV block)
- Neural involvement : cranial and peripheral palsies
Rhodococcus equi (Corynebacterium equi) classification shape colonize causes
non-spore, non-mobile bacilli
clubbed rod
mammals and soil
**pulmonary disease in immunocompromized ** + lung abscess, pneumonia **
Rhodococcus equi (Corynebacterium equi) what can you see
X-ray : upper lung nodules, cavities with air-fluid levels
Listeria Monocytogenes classification shape colonizes virulence factor
non-spore, mobile, anaerobic bacilli
rod + flagella
cooler temperatures, DAIRY, MEAT, sprouts contaminated
Listeriolysion O, macrophage phagolysosome escape
Listeria Monocytogenes effects more
10X more in pregnant women (advised not to eat products at risk)
Listeria Monocytogenes at higher temperatures
flagella tumbling motility
Listeria Monocytogenes SX in immunocompotents patients
mild flu like , gasteroenteritis (fever, headache, N,V, D) = Listeriosis
Listeria Monocytogenes SX in neonate and fetus
- Granulomatous infantiseptica : granulomas in multiple organs, fatal,
- Neonatal Meningitis : due to fecal contanimation (2-3 weeks after birth)
what 2 organisms can cause sepsis to pregnant women
- Listeria Monocytogenesis
2. Streptococcus Group B (agalactiae)
Listeria Monocytogenes in immunocompromized patients
can cause meningitis (or healthy older adults) , or patients taking immunosuppressants like corticosteroids
3 types of diplococci gram 1 bacteria
- Neisseria Meningitis
- Neisseria Gonorrhea
- Moraxella Catarrhalis
Neisseria Meningitis
grows on
colonizes
virulence factor
heated blood agar (“chocolate agar”)
= Thayer-Martin agar, VCN Ab modified
colonizes nasopharynx
LPS endotoxin, capsule, IgA protease, pili
Neisseria Meningitis susceptible to
- Neonates
- Military recruits
- College student
- Asplenia (hyposplenia)
chocolate agar
heated lysed blood agar
vancomycin kills
gram +
Polymyxin kills
gram - except Neisseria
nystatin kills
fungi
Neisseria Meningitis has what toxin
endotoxin —-> fulminate meningococcal sepsis
Neisseria Meningitis SX
- Meningitis : stiff neck , V,N, rash (small red purple lesions)
- Meningococcemia : meningitis + specticemia
Neisseria Meningitis Meningococcemia
Waterhouse-Friderichsen syndrome = bilateral adrenal hemorrhage + insufficiency, severe hypotension**, disseminated intravascular thrombosis, death
Neisseria gonorrhoeae
classification
grows on
gram -, diplococci
VCN (Thayer-Martin chocolate agar)
Neisseria gonorrhoeae virulence factors
IgA protease
Pili (adherence)
Opa (prevent immune)
Neisseria gonorrhoeae SX
- Urethritis (dyuria, discharge)
- Acute epididymis (post testicular pain+ swelling)
- Cervicitis : itching, discharge, ascending
- Pelvic inflammatory disease = infection of uterus, fallopian tube, ovaries (pain)
Pelvic inflammatory disease can cause
Fitz-Hugh Curtis syndrome
= perihepatits (inflammation of liver capsule, RUQ PAIN)
- can be from Neisseria gonorrhoeae
Neisseria gonorrhoeae SX in infants
ophthalamia neonatorum = neonatal conjunctivitis
Neisseria gonorrhoeae can rarely cause what
septic arthritis
Moraxella catarrhalis
classification
grows on
colonizes
gram - diplococci
chocolate agar + routine blood agar
normal respiratory flora
Moraxella catarrhalis can cause what in
elderly
children
bronchopneumonia otis media (3rd common cause)****
Enteric bacteria are classified as
4 classes
gram - part of normal GI flora
- Enterobacteriacease
- Pseudomonadaceae
- Bacteriodaceae
- Vibronaceae
Watery Diarrhea means
X invasion
- Enterotoxigenic E. coli
- Vibro cholera
Bloody Diarrhea, fever, ABD cramps means
intestinal epithelial invasion —-> death
- Enterohemorrhagic E. coli
- Enteroinvasive E. coli
- Shigella
Bloody or NO bloody (watery) Diarrhea, fever, ABD pain —-> sepsis, bacterimia means
Invasion of LNs or BS
- Salmonella typhi
- Yersinia enterocolitica
- Campylobacter jejuni
what organisms cause GI disease
- E. Coli (UTI, D, pneumonia, neonatal meningitis)
- Shigella
- Salmonella typhi (typhoid fever, osteomyelitis)
- Yersinia enterocolitica
what organisms cause nosocomial infections
- Proteus mirabilis (UTI)
- Klebsiella pneumoniae (pneumonia)
- Enterobacter Serratia (UTI, pneumonia)
- E. coli (UTI)
nosocomial infections
from hospital, nursing home, institutional care (UTI, pneumonia)
what organisms ferment lactose
where do they grow (what type of agar)
E. COLI, and many nosocomial Enterobacteriaceae
- PINK : on MacConkey agar**
- Dark black with metallic sheen : on EMB agar
E. coli
classification
colonization
virulence factor
gram - bacilli
normal flora in GI
gets virulence factor from plasmid exchange or bacteriophage
E. coli SX
D
Meningitis if neonatal
UTI
Pneumonia
Enterotoxigenic E. coli SX and most common places
watery D + ADB cramps = travelers diarrhea
Mexico, Middle east, Africa, South America, Asia
Enterotoxigenic E. coli
toxins
LT and ST toxins pulling water out + X NaCl or ion reabsorption
Enteroinvasive E. coli usually contracted from
contaminated food
invades bowel = bloody D +fever
Enteroinvasive E. coli is similar to
Shigella
Enterotoxigenic E. coli is similar to
Cholera
Enterohemorrhagic E. coli
toxins
other name for it
Shiga-like toxin = stx1 stx2 : cell death
E. coli 0157
what does E. coli 0157 cause
EHEC
- Hemolytic uremic syndrome : anemia thrombopenia, renal blood and damage = STX2*
- Hemorrhagic colitis : bloody D, ABD cramps, fever
E. coli causes
in neonatal
in most women
in hospital
meningitis
UTI
pneumonia (usually weak patients)
Shigella
classification
colonizes
most common place to get it
gram - bacilli, non-motile
GI
preschool, nursing home, fecal oral transmission
Shigella SX
most common in US type
more ever type
HIGH FEVER, BLOODY D, abd cramps
S. sonnei (US)
S. dysenteriae in more severe (cell death by —-I 60S ribosome subunit)
Shigella toxin
Shiga toxin
Salmonella
classification
how do you get it
can cause or develop to what
gram - bacilli, motile, encapsulated
fecal oral transmission, contaminated food (chicken, egg, spinach)
chronic carriage in gallbladder = gallbladder adenocarcinoma (only humans)
Salmonella typhi
thyphoid fever, only humans
- rose spots = bacteria emboli
- HIGH fever (STEPWISE**)
- diffuse colicky RUQ pain
- bloody D
- Delirium (typhoid encephalopathy)
Salmonella non-thyphoidal
in animals (handling hedgehogs, turtles)
- N, V
- WATERY D* (blood or no blood)
- fever
Salmonella toxin
BIOFILM (which can let it hide in gallbladder)
Salmonella invade through
intestine
stepwise fever
raises at day drops each morning
each raise getting higher
rose sports can also be described as
erythmatous maculopapules
Yersinia enterocolitica
classification
colonizes in
gram - bacilli, motile, bipolar stain
mammals, contaminated food/water (MILK)
Yersinia enterocolitica SX
- fever
- ABD pain
- D
- Pseudoappendicitis (RLQ pain)
Proteus mirabilis
classification
what toxin
gram - bacilli, MOTILE (swarms) = concentric rings
urease = breaks down urea —-> CO2 + NH4+
= alkaline urine
= pink in urase test
Proteus mirabilis can have a reaction to what other organism
Rickettsia
Proteus mirabilis causes
UTI esp in nosocomial setting
Klebsiella pneumoniae
classification
common to who
gram - bacilli, ENCAPSULATED
alcoholics + hospitilized patients
Klebsiella pneumoniae SX
pneumonia necrotizing = “currant jelly” sputum
can cause UTI (esp. with catheter)
high mortality rate
Serratia looks
red on agar
grows in damp bathrooms, toilets and areas like that