Bacteria (Bugs in System) Flashcards
1
Q
Describe the process of gram staining:
A
+ve crystal violet binds to -ve components
iodine complexes with crystal violet
acetone/methanol solution washes these out in from -ve cells
red dye then stains the now unstained gram -ve bacteria
2
Q
Describe a gram +ve cell:
A
thick peptidoglycan wall
retains dye
3
Q
Describe a gram -ve cell
A
thin peptidoglycan layer between two membranes
loses dye
4
Q
What are the 5 common bacterial shapes?
A
cocci diplococci streptococci staphylococci bacilli
5
Q
Name some gram +ve cocci:
A
Staphylococcus
Streptococcus
6
Q
Name some gram +ve bacilli
A
Clostridium
7
Q
Name some gram -ve cocci
A
Neisseria
8
Q
Name some gram -ve bacilli
A
Salmonella Helicobacter Legionella Bacteriodes Escheria Pseudomonas
9
Q
Which bacteria cannot be gram stained?
A
Mycobacteria - cell wall
Mycoplasmas - no peptidoglycan
Chlamydia, rickesettia - too small
10
Q
What stain would be used on mycobacteria?
A
acid fast
