Bacteria

Question 1

List genus for gram + bacteria

Answer 1

• Staphylococcus

- streptococcus

Question 2

List genus for gram -ve bacteria

Answer 2

• Neisseria
• moraxella
• kingella

Question 3

What can neisseria cause?

Answer 3

• n. meningitidis

- n. gnorrhoaea

Question 4

What can streptococcus cause?

Answer 4

• s. pyrogens
• s. agalactiae
• s. pneumoniae
• enteroccocus

Question 5

What can staphylococcus cause?

Answer 5

• s. aureas
  (coagulase negative)
  –> it causes TSS!

Question 6

What is the source of endotoxins?

Answer 6

Gram -ve bacterial cell wall

Question 7

What method is used to cultivate listeria monocytogenes?

Answer 7

beta hemolysis on blood agar

Question 8

Listeria monocytogenes is not catalase +. T/F?

Answer 8

False!

It IS catalase positive!

Question 9

Is listeria monocytogenes CAMP positive?

Answer 9

Yes!

Question 10

What is the germ number?

Answer 10

The number of all bacterial/fungal cells in a culture, water or food sample, vaccine.

Question 11

What is the colony forming unit (CFU/ML)?

Answer 11

The number of living microorganisms in a certain sample.

Question 12

List methods of germ-count:

Answer 12

• spectrophotometry

- electrical particle counter (flow cytometry)

Question 13

List methods of counting colony forming units:

Answer 13

• pouring agar plates

- germ-count from serial dilution

Question 14

What are the physical methods of anaerobic cultivation?

Answer 14

• anaerostat (airtight metal cylinder)
• high agar: inoculation to bottom of medium
• liquid culture media