Bacteria Flashcards
what colour is gram positive vs negative
positive = purple - stay positive, stay purple!
negative = pink
name gram positive cocci
staphylococci
streptococci
including enterococci
name gram negative cocci
neisseria meningitidis
neisseria gonorrhoeae
moxarella catarrhalis
name gram positive bacilli
ABCDL
Actinomyces
Bacillus anthracis (anthrax)
Clostridium
Diptheria (corynebacterium diptheriae)
Listeria monocytogenes
name gram negative bacilli
Everything else
E.coli
Haemophilus influenzae
Pseudomonas aerginosa
Salnmonella
Shigella
Campylobacter jejuni (curved shape)
Vibro cholerae
how to differentiate between streptococci?
a/b/y hemolysis of blood Agar
A= partial so green
strep pneumoniae
Strep Viridiana
B= complete haemolysis so clear
Lancefield group ABCDG
A- strep progenies
B- agaltiate
C-
D- strep galcolycticus and enterococci
G-
Y= no hameolysis
strep milleri
How to differentiate staph aureus from coagulase-negative staphylococci?
S.aureus is gold on colonies on blood agar
S.aureus is coagulase test positive = clot
Name a coagulase-negative staphylococci
Staph epidermidis
What organisms are lactose fermenting? How can you tell
E.coli
Staphylococcus saprophyticus
CLED - yellow colonies
Macockney = pink
yellow and pink
What organisms are non-lactose fermenting? How can you tell?
Pseudomonas
Shigella
Salmonella
CLED - blue colonies
MacCockney - yellow/colourless
blue and yellow
How can you tell the difference between salmonella and shigella?
XLD agar (red plate)
Salmonella = yellow then pink then black centres
Shigella = remain pink/red
What shape is campylobacter jejuni?
curved rod
what class of abx is goof for CNS infections
cephalosporin beta lactams eg ceftriaxone/cefotaxime = They have very good CNS penetration that are active against gram positive cocci. They have a broader spectrum than some of the penicillins making them suitable before the organism’s identify and antibiotic sensitivity profile is determined.
what abx are needed if listeria is suspected in a CNS infection
Amoxicillin +/- gentamicin
Listeria is not susceptible to cephalosporins
UTI bacterial causes
E.coli
Proteus mirabilis
Klebsiella spp.
Staphylococcus saprophyticus
Staphylococcus epidermidis
Enterococci