BACTE MYCOBACTERIA Flashcards
Bacteria that are:
-strictly aerobic, non-spore forming
-slender, slightly curved, slow-growing bacilli
-non-motile, catalase positive, non-encapsulated, Much’s granules
MYCOBACTERIA
Most pathogenic species of Mycobacteria require ____________________ of incubation at _____ (temp)
2-6 weeks, 35-37 °C
MYCOBACTERIUM TUBERCULOSIS is also known as
Koch’s bacillus or human tubercle bacilli
Agent of tuberculosis
MYCOBACTERIUM TUBERCULOSIS
Doubling time of MYCOBACTERIUM TUBERCULOSIS
729-932 minutes
______ number of sputum specimens should be obtained for Direct Sputum Smear Microscopy
Two (2)
ATLEAST ____ SPUTUMSMEARPOSITIVEIS CONSIDERED BACTERIOLOGICALLY CONFIRMED TB.
ONE
Bacteria wherein the Colonies are typically raised, with dry(cauliflower-like) rough appearance on egg-based media
MYCOBACTERIUM TUBERCULOSIS
Ideal temp for growth of M. tuberculosis
35 - 37 deg C
On Lowenstein-Jensen (LJ) slants, M.tb typically has a crumbly, ____________ appearance, whereas M. avium complex has a bright yellow, smooth, characteristic colony so-called “_________________”
“bread-crumb”
“condom colony”
o Original test reagent for the tuberculin test
o Prepared from boiled 6-week old broth cultures, from which the organisms were filtered and concentrated by steaming
o the active component of the filtrate is a heat-stable protein
Old Tuberculin (OT)
intracutaneous injection way of testing M. tb
Mantoux test
scratching the tuberculin
into the skin way of testing M. tb
Von pirquet test
piece of cloth soaked in OT/PPD and placed over skin, way of testing M. tb
Vollmer patch test
OT/PPD + lanolin-ointment rubbed onto skin, way of testing M. tb
Moro percutaneous test
multiple puncture technique way of testing M. tb
Tuberculin tine test
Skin test which identifies tuberculosis
infection, recent or past, with or without
disease
Tuberculin Test
INTERPRETATION OF RESULTS for Tuberculin Test
Greater than or equal to 15mm
Positive individuals with no risk factors
INTERPRETATION OF RESULTS for Tuberculin Test:
Positive in recent immigrants of high prevalence countries,
IV drug users, employees of healthcare
and other high-risk facilities, children
younger than 5 years of age
10 mm or greater
INTERPRETATION OF RESULTS for Tuberculin Test:
Positive in persons who
have HIV infection, immunosuppressive
disorders, features on a chest X-ray
consistent with TB, or recent contact with
TB patients
5 mm or more
samples only for patients who do not produces sputum by aerosol induction, for children younger than 12 years, and for non-ambulatory individuals
GASTRICASPIRATES
preferred over BAL for detectionofMTBin children
obtained in the morning after overnight fasting 3 specimens arecollectedwithin 3 days
GASTRICLAVAGE
Specimens should be centrifuged at either
____________________ or 2__________________
3,000Xg for 15 minutes , ,000Xg for
20 minutes
Used for decontamination of sputum
specimens that contain Gram-negative
rods like Pseudomonas aeruginosa
5% Oxalic Acid
