AFB

Question 1

Reliable approach for identifying acid fast bacteria

Answer 1

Ziehl- Neelsen method

Question 2

Ziehl- Neelsen method primary stain

Answer 2

BASIC FUCHSIN

Question 3

Ziehl- Neelsen method mordant

Answer 3

Carbolic acid (Phenol)

Question 4

also known the as the Hot Staining Method

Answer 4

Ziehl- Neelsen method

Question 5

also known the as the Cold Staining Method

Answer 5

Kinyoun’s Modified Acid Fast Stain

Question 6

is used to detect bacteria that possess acid-fast characteristics

Answer 6

Acid Fast Stain

Question 7

Acid Fast Stain is critical for diagnosing what disease?

Answer 7

tuberculosis

Question 8

Acid Fast Stain can also highlight the cysts of ____________________________ and __________________

Answer 8

Cryptosporidium and Isospora

Question 9

also known the as the Truant Staining Method

Answer 9

Auramine- Rhodamine Fluorochrome

Question 10

Ziehl- Neelsen and Kinyoun methods can be visualized using a __________________________

Answer 10

Standard Bright-field Microscope

Question 11

Auramine- Rhodamine Fluorochrome staining method requires a _________________________ to be visualized

Answer 11

Fluorescent Microscope

Question 12

Acid Fast Staining requires higher ___________________________ to allow penetration

Answer 12

Dye concentrations and/ or heating

Question 13

Bacteria that retain the primary stain (carbolfuchsin)

Answer 13

Acid Fast Bacteria

Question 14

Bacteria that lose the primary stain after decolorization and take up the counterstain

Answer 14

Non Acid Fast Bacteria

Question 15

Acid Fast Staining counterstain

Answer 15

Methylene Blue

Question 16

color of Acid Fast Bacteria

Answer 16

RED

Question 17

color of Non- Acid Fast Bacteria

Answer 17

BLUE

Question 18

How many fields should be examined before ruling out acid fast bacteria

Answer 18

300 Fields