AFB Flashcards
Reliable approach for identifying acid fast bacteria
Ziehl- Neelsen method
Ziehl- Neelsen method primary stain
BASIC FUCHSIN
Ziehl- Neelsen method mordant
Carbolic acid (Phenol)
also known the as the Hot Staining Method
Ziehl- Neelsen method
also known the as the Cold Staining Method
Kinyoun’s Modified Acid Fast Stain
is used to detect bacteria that possess acid-fast characteristics
Acid Fast Stain
Acid Fast Stain is critical for diagnosing what disease?
tuberculosis
Acid Fast Stain can also highlight the cysts of ____________________________ and __________________
Cryptosporidium and Isospora
also known the as the Truant Staining Method
Auramine- Rhodamine Fluorochrome
Ziehl- Neelsen and Kinyoun methods can be visualized using a __________________________
Standard Bright-field Microscope
Auramine- Rhodamine Fluorochrome staining method requires a _________________________ to be visualized
Fluorescent Microscope
Acid Fast Staining requires higher ___________________________ to allow penetration
Dye concentrations and/ or heating
Bacteria that retain the primary stain (carbolfuchsin)
Acid Fast Bacteria
Bacteria that lose the primary stain after decolorization and take up the counterstain
Non Acid Fast Bacteria
Acid Fast Staining counterstain
Methylene Blue
color of Acid Fast Bacteria
RED
color of Non- Acid Fast Bacteria
BLUE
How many fields should be examined before ruling out acid fast bacteria
300 Fields
Allows acid fast bacilli to resist staining
Thick Mycolic Acid wall
ZIEHL NEELSEN Method:
- STAINING PRINCIPLE
- MORDANT
- TIME
- Carbol Fuchsin
- Heat
- Time Consuming
KINYOUN Method:
- STAINING PRINCIPLE
- MORDANT
- TIME
- Carbol Fuchsin
- Phenol
- faster than Ziehl Neelsen
AURAMINE RHODAMINE Method:
- STAINING PRINCIPLE
- MORDANT
- TIME
- Fluorescent Dye
- Does not require mordant
- Quick observation of Fluorescence under UV
